AB23938

Anti-TIM50 antibody

Name: Anti-TIM50 antibody (ab23938) | Abcam
Brand: Abcam Limited
SKU: AB23938
Price: 545 USD
Availability: InStock
Rating: 4 (4 reviews)

(4 Reviews)

(9 Publications)

Goat Polyclonal TIM50 antibody. Suitable for WB and reacts with Human samples. Cited in 9 publications. Immunogen corresponding to Synthetic Peptide within Human TIMM50 aa 250-350.

View Alternative Names

TIM50, PRO1512, TIMM50, Mitochondrial import inner membrane translocase subunit TIM50

Key facts

Host species

Goat

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

applications

Immunogen

Synthetic Peptide within Human TIMM50 aa 250-350. The exact immunogen used to generate this antibody is proprietary information.

Q3ZCQ8

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Reactivity data

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

Purified by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Storage buffer

pH: 7.3 Preservative: 0.02% Sodium azide Constituents: Tris buffered saline, 0.5% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

TIM50 also known as Translocase of Inner Mitochondrial Membrane 50 is a protein weighing approximately 50 kDa. This protein plays an important mechanical role in the mitochondrial presequence translocase functioning as a receptor and import channel within the inner mitochondrial membrane. TIM50 is expressed predominantly within the inner membranes of mitochondria in eukaryotic cells.

Biological function summary

TIM50 facilitates the import of presequence-containing proteins from the cytosol into the mitochondrial matrix acting as part of the TIM23 complex. This complex orchestrates the translocation of proteins across the inner mitochondrial membrane. By interacting with presequences of nuclear-encoded mitochondrial proteins TIM50 ensures proper import and processing which is important for mitochondrial function and biogenesis.

Pathways

TIM50 is critical in the mitochondrial protein import pathway which is essential for mitochondrial respiration and energy production. TIM50 interacts with other proteins such as TIM23 and TIM17 to form the translocase of the inner membrane 23 (TIM23) complex. TIM50 also links to the mitochondrial unfolded protein response (UPR) where it may influence the regulation of proteostasis within mitochondria alongside proteins like HSP70.

Mutations and dysregulation of TIM50 have connections to mitochondrial diseases which encompass a range of disorders due to mitochondrial dysfunction. Additionally TIM50 may affect neurodegenerative diseases where mitochondrial impairment plays a role. Alterations in TIM50 influencing mitochondrial function might correlate with proteins like cytochrome c which can contribute to apoptotic pathways and cell death in these diseases.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Essential component of the TIM23 complex, a complex that mediates the translocation of transit peptide-containing proteins across the mitochondrial inner membrane (PubMed : 30190335, PubMed : 38828998). Has some phosphatase activity in vitro; however such activity may not be relevant in vivo.. Isoform 2. May participate in the release of snRNPs and SMN from the Cajal body.

See full target information TIMM50

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Publications (9)

Recent publications for all applications. Explore the full list and refine your search

Anatomical record (Hoboken, N.J. : 2007) 305:209-221 PubMed34041863

2021

Outer and inner mitochondrial membrane proteins TOMM40 and TIMM50 are intensively concentrated and localized at Purkinje and pyramidal neurons in the New Zealand white rabbit brain.

Applications

Unspecified application

Species

Unspecified reactive species

Sameh M Farouk,Ahmed M Abdellatif,Elsayed Metwally

Journal of hepatology 68:1228-1238 PubMed29408314

2018

Fibroblast growth factor receptor inhibition induces loss of matrix MCL1 and necrosis in cholangiocarcinoma.

Applications

Species

Unspecified reactive species

Ayano Kabashima,Petra Hirsova,Steven F Bronk,Matthew C Hernandez,Mark J Truty,Sumera Rizvi,Scott H Kaufmann,Gregory J Gores

Molecules (Basel, Switzerland) 22: PubMed28786914

2017

Artesunate Activates the Intrinsic Apoptosis of HCT116 Cells through the Suppression of Fatty Acid Synthesis and the NF-κB Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Xiao Chen,Yin Kwan Wong,Teck Kwang Lim,Wei Hou Lim,Qingsong Lin,Jigang Wang,Zichun Hua

Circulation research 120:99-109 PubMed27777247

2016

AMP-Activated Protein Kinase α2 in Neutrophils Regulates Vascular Repair via Hypoxia-Inducible Factor-1α and a Network of Proteins Affecting Metabolism and Apoptosis.

Applications

Species

Mouse

Randa Abdel Malik,Nina Zippel,Timo Frömel,Juliana Heidler,Sven Zukunft,Barbara Walzog,Nariman Ansari,Francesco Pampaloni,Susanne Wingert,Michael A Rieger,Ilka Wittig,Beate Fisslthaler,Ingrid Fleming

Cell death and differentiation 22:959-73 PubMed25361083

2014

CR6-interacting factor 1 is a key regulator in Aβ-induced mitochondrial disruption and pathogenesis of Alzheimer's disease.

Applications

Species

Mouse

J Byun,S M Son,M-Y Cha,M Shong,Y J Hwang,Y Kim,H Ryu,M Moon,K-S Kim,I Mook-Jung

American journal of physiology. Regulatory, integr 304:R553-65 PubMed23408027

2013

Reversal of mitochondrial proteomic loss in Type 1 diabetic heart with overexpression of phospholipid hydroperoxide glutathione peroxidase.

Applications

Unspecified application

Species

Unspecified reactive species

Walter A Baseler,Erinne R Dabkowski,Rajaganapathi Jagannathan,Dharendra Thapa,Cody E Nichols,Danielle L Shepherd,Tara L Croston,Matthew Powell,Trust T Razunguzwa,Sara E Lewis,David M Schnell,John M Hollander

Nature cell biology 14:575-83 PubMed22544066

2012

Anti-apoptotic MCL-1 localizes to the mitochondrial matrix and couples mitochondrial fusion to respiration.

Applications

Unspecified application

Species

Unspecified reactive species

Rhonda M Perciavalle,Daniel P Stewart,Brian Koss,John Lynch,Sandra Milasta,Madhavi Bathina,Jamshid Temirov,Megan M Cleland,Stéphane Pelletier,John D Schuetz,Richard J Youle,Douglas R Green,Joseph T Opferman

Glycobiology 20:148-57 PubMed19797320

2009

Human sialidase NEU4 long and short are extrinsic proteins bound to outer mitochondrial membrane and the endoplasmic reticulum, respectively.

Applications

Species

Human

Alessandra Bigi,Lavinia Morosi,Chiara Pozzi,Matilde Forcella,Guido Tettamanti,Bruno Venerando,Eugenio Monti,Paola Fusi

Cell death and differentiation 15:545-54 PubMed18174902

2008

Intra-mitochondrial degradation of Tim23 curtails the survival of cells rescued from apoptosis by caspase inhibitors.

Applications

Species

Unspecified reactive species

C G Goemans,P Boya,C J Skirrow,A M Tolkovsky

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-TIM50 antibody

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (9)

Product promise