JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB109436

Anti-TIM50 antibody [EPR5785]

5

(2 Reviews)

|

(5 Publications)

Rabbit Recombinant Monoclonal TIM50 antibody. Suitable for WB and reacts with Human, Mouse, Rat samples. Cited in 5 publications.

View Alternative Names

TIM50, PRO1512, TIMM50, Mitochondrial import inner membrane translocase subunit TIM50

2 Images
Western blot - Anti-TIM50 antibody [EPR5785] (AB109436)
  • WB

Unknown

Western blot - Anti-TIM50 antibody [EPR5785] (AB109436)

All lanes:

Western blot - Anti-TIM50 antibody [EPR5785] (ab109436) at 1/1000 dilution

Lane 1:

HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 10 µg

Lane 2:

SH-SY5Y (Human neuroblastoma cell line from bone marrow) whole cell lysate at 10 µg

Lane 3:

Hep G2 (Human liver hepatocellular carcinoma cell line) whole cell lysate at 10 µg

Lane 4:

HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate at 10 µg

Predicted band size: 40 kDa

false

OI-RD Scanning - Anti-TIM50 antibody [EPR5785] (AB109436)
  • OI-RD Scanning

Unknown

OI-RD Scanning - Anti-TIM50 antibody [EPR5785] (AB109436)

We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody. Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.

  • Carrier free

    Anti-TIM50 antibody [EPR5785] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR5785

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Mouse": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
style
left-column
style
left-column

Product details

style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.05% Sodium azide Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 0.1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

TIM50 also known as Translocase of Inner Mitochondrial Membrane 50 is a protein weighing approximately 50 kDa. This protein plays an important mechanical role in the mitochondrial presequence translocase functioning as a receptor and import channel within the inner mitochondrial membrane. TIM50 is expressed predominantly within the inner membranes of mitochondria in eukaryotic cells.
Biological function summary

TIM50 facilitates the import of presequence-containing proteins from the cytosol into the mitochondrial matrix acting as part of the TIM23 complex. This complex orchestrates the translocation of proteins across the inner mitochondrial membrane. By interacting with presequences of nuclear-encoded mitochondrial proteins TIM50 ensures proper import and processing which is important for mitochondrial function and biogenesis.

Pathways

TIM50 is critical in the mitochondrial protein import pathway which is essential for mitochondrial respiration and energy production. TIM50 interacts with other proteins such as TIM23 and TIM17 to form the translocase of the inner membrane 23 (TIM23) complex. TIM50 also links to the mitochondrial unfolded protein response (UPR) where it may influence the regulation of proteostasis within mitochondria alongside proteins like HSP70.

Mutations and dysregulation of TIM50 have connections to mitochondrial diseases which encompass a range of disorders due to mitochondrial dysfunction. Additionally TIM50 may affect neurodegenerative diseases where mitochondrial impairment plays a role. Alterations in TIM50 influencing mitochondrial function might correlate with proteins like cytochrome c which can contribute to apoptotic pathways and cell death in these diseases.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Essential component of the TIM23 complex, a complex that mediates the translocation of transit peptide-containing proteins across the mitochondrial inner membrane. Has some phosphatase activity in vitro; however such activity may not be relevant in vivo.. Isoform 2. May participate in the release of snRNPs and SMN from the Cajal body.
See full target information TIMM50
style
left-column

Publications (5)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in immunology 13:935710 PubMed36451821

2022

The mitochondrial gene-CMPK2 functions as a rheostat for macrophage homeostasis.

Applications

Unspecified application

Species

Unspecified reactive species

Prabhakar Arumugam,Meghna Chauhan,Thejaswitha Rajeev,Rahul Chakraborty,Kanika Bisht,Mahima Madan,Deepthi Shankaran,Sivaprakash Ramalingam,Sheetal Gandotra,Vivek Rao

The Journal of experimental medicine 219: PubMed35616614

2022

MPST sulfurtransferase maintains mitochondrial protein import and cellular bioenergetics to attenuate obesity.

Applications

Unspecified application

Species

Unspecified reactive species

Antonia Katsouda,Dimitrios Valakos,Vasilios S Dionellis,Sofia-Iris Bibli,Ioannis Akoumianakis,Sevasti Karaliota,Karim Zuhra,Ingrid Fleming,Noriyuki Nagahara,Sophia Havaki,Vassilis G Gorgoulis,Dimitris Thanos,Charalambos Antoniades,Csaba Szabo,Andreas Papapetropoulos

The journal of physiological sciences : JPS 69:359-373 PubMed30570704

2018

Regulation of mitochondrial iron homeostasis by sideroflexin 2.

Applications

Unspecified application

Species

Unspecified reactive species

Ei Ei Mon,Fan-Yan Wei,Raja Norazireen Raja Ahmad,Takahiro Yamamoto,Toshiro Moroishi,Kazuhito Tomizawa

Human molecular genetics 27:3881-3900 PubMed30084972

2018

CHCHD2 accumulates in distressed mitochondria and facilitates oligomerization of CHCHD10.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaoping Huang,Beverly P Wu,Diana Nguyen,Yi-Ting Liu,Melika Marani,Jürgen Hench,Paule Bénit,Vera Kozjak-Pavlovic,Pierre Rustin,Stephan Frank,Derek P Narendra

Nature neuroscience 17:822-31 PubMed24836077

2014

Inhibition of mitochondrial protein import by mutant huntingtin.

Applications

Unspecified application

Species

Mouse

Hiroko Yano,Sergei V Baranov,Oxana V Baranova,Jinho Kim,Yanchun Pan,Svitlana Yablonska,Diane L Carlisle,Robert J Ferrante,Albert H Kim,Robert M Friedlander
View all publications
style
left-column
style
left-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions
style
left-column
style
right-column

Associated Products

Select an associated product type
Alternative Product
Primary Antibodies

AB23938

Anti-TIM50 antibody

primary-antibodies

tim50-antibody-ab23938

4

(4 reviews)

Alternative Product
Primary Antibodies

AB204486

Anti-TIM50 antibody

primary-antibodies

tim50-antibody-ab204486

0

(0 reviews)

Alternative Product
Primary Antibodies

AB109527

Anti-TIM50 antibody [EPR5784]

primary-antibodies

tim50-antibody-epr5784-ab109527

0

(0 reviews)

style
bg-slate-100

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com