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AB229218

Anti-Timeless antibody

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(2 Publications)

Rabbit Polyclonal Timeless antibody. Suitable for IP, WB, ICC/IF and reacts with Human, Mouse samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human TIMELESS.

View Alternative Names

TIM, TIM1, TIMELESS1, TIMELESS, Protein timeless homolog, hTIM

4 Images
Immunocytochemistry/ Immunofluorescence - Anti-Timeless antibody (AB229218)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Timeless antibody (AB229218)

4% paraformaldehyde-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for Timeless (green) using ab229218 at 1/2000 dilution in ICC/IF.

Nuclear counterstain : Hoechst 33342 (blue).

Immunoprecipitation - Anti-Timeless antibody (AB229218)
  • IP

Supplier Data

Immunoprecipitation - Anti-Timeless antibody (AB229218)

Timeless was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract with 5 μg of ab229218. Western blot was performed from the immunoprecipitate using ab229218. Anti-Rabbit IgG was used as a secondary reagent.

Lane 1 : HEK-293T whole cell extract (Input).
Lane 2 : Control IgG instead of ab229218 in HEK-293T whole cell extract.
Lane 3 : ab229218 IP in HEK-293T whole cell extract.

All lanes:

Immunoprecipitation - Anti-Timeless antibody (ab229218)

Predicted band size: 139 kDa

false

Western blot - Anti-Timeless antibody (AB229218)
  • WB

Supplier Data

Western blot - Anti-Timeless antibody (AB229218)

5% SDS-PAGE gel.

All lanes:

Western blot - Anti-Timeless antibody (ab229218) at 1/1000 dilution

Lane 1:

HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 30 µg

Lane 2:

A431 (human epidermoid carcinoma cell line) whole cell lysate at 30 µg

Lane 3:

HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 30 µg

Lane 4:

HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate at 30 µg

Predicted band size: 139 kDa

false

Western blot - Anti-Timeless antibody (AB229218)
  • WB

Supplier Data

Western blot - Anti-Timeless antibody (AB229218)

5% SDS-PAGE gel.

All lanes:

Western blot - Anti-Timeless antibody (ab229218) at 1/1000 dilution

Lane 1:

Neuro-2a (mouse neuroblastoma cell line) whole cell extract at 30 µg

Lane 2:

C8D30 whole cell extract at 30 µg

Lane 3:

NIH/3T3 (mouse embryonic fibroblast cell line) whole cell extract at 30 µg

Lane 4:

RAW 264.7 (mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell extract at 30 µg

Lane 5:

C2C12 (mouse myoblast cell line) whole cell extract at 30 µg

Predicted band size: 139 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Human

Applications

IP, WB, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human TIMELESS. The exact immunogen used to generate this antibody is proprietary information.

Q9UNS1

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/100 - 1/500", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/2000", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 Preservative: 0.025% Proclin 300 Constituents: PBS, 20% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Timeless also known as timeless circadian regulator is a protein with a molecular mass of about 139 kDa. It plays an important role in the stabilization of the circadian rhythm in mammals. Timeless associates with other clock proteins and participates in the feedback loop that drives circadian oscillations influencing hormonal and metabolic pathways. Researchers find Timeless expression primarily in the suprachiasmatic nucleus of the brain the central circadian pacemaker but it is also present in peripheral tissues.
Biological function summary

The protein influences the circadian rhythm by interacting with CLOCK and BMAL1 forming part of a macromolecular complex. Timeless helps sustain the proper timing and expression of various genes influenced by the circadian rhythm ensuring an orderly and synchronized cellular process across tissues. Its interaction with other clock components regulates circadian-controlled pathways governing processes like sleep-wake cycles hormone release and cell metabolism.

Pathways

Timeless participates in the regulation of the circadian rhythm and the cell cycle. It interacts with PER1 and PER2 proteins central to the inhibition of the CLOCK/BMAL1 complex which plays a pivotal role in maintaining the circadian clock's precision. Timeless also interfaces with proteins involved in DNA damage response pathways promoting effective DNA repair during cell division and aligning cell cycle regulation with circadian rhythms.

Timeless has been linked to cancer and sleep disorders. The protein's role in DNA repair and cell cycle control associates it with tumorigenesis when its function becomes dysregulated. Additionally disruptions in Timeless expression or function are connected to sleep phase disorders highlighting its involvement in maintaining proper circadian function. In cancer alterations in Timeless could lead to interactions with oncogenes or tumor suppressors potentially affecting cancer progression.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Plays an important role in the control of DNA replication, maintenance of replication fork stability, maintenance of genome stability throughout normal DNA replication, DNA repair and in the regulation of the circadian clock (PubMed : 17141802, PubMed : 17296725, PubMed : 23359676, PubMed : 23418588, PubMed : 26344098, PubMed : 31138685, PubMed : 32705708, PubMed : 35585232, PubMed : 9856465). Required to stabilize replication forks during DNA replication by forming a complex with TIPIN : this complex regulates DNA replication processes under both normal and stress conditions, stabilizes replication forks and influences both CHEK1 phosphorylation and the intra-S phase checkpoint in response to genotoxic stress (PubMed : 17141802, PubMed : 17296725, PubMed : 23359676, PubMed : 35585232). During DNA replication, inhibits the CMG complex ATPase activity and activates DNA polymerases catalytic activities, coupling DNA unwinding and DNA synthesis (PubMed : 23359676). TIMELESS promotes TIPIN nuclear localization (PubMed : 17141802, PubMed : 17296725). Plays a role in maintaining processive DNA replication past genomic guanine-rich DNA sequences that form G-quadruplex (G4) structures, possibly together with DDX1 (PubMed : 32705708). Involved in cell survival after DNA damage or replication stress by promoting DNA repair (PubMed : 17141802, PubMed : 17296725, PubMed : 26344098, PubMed : 30356214). In response to double-strand breaks (DSBs), accumulates at DNA damage sites and promotes homologous recombination repair via its interaction with PARP1 (PubMed : 26344098, PubMed : 30356214, PubMed : 31138685). May be specifically required for the ATR-CHEK1 pathway in the replication checkpoint induced by hydroxyurea or ultraviolet light (PubMed : 15798197). Involved in the determination of period length and in the DNA damage-dependent phase advancing of the circadian clock (PubMed : 23418588, PubMed : 31138685). Negatively regulates CLOCK|NPAS2-ARTNL/BMAL1|ARTNL2/BMAL2-induced transactivation of PER1 possibly via translocation of PER1 into the nucleus (PubMed : 31138685, PubMed : 9856465). May play a role as destabilizer of the PER2-CRY2 complex (PubMed : 31138685). May also play an important role in epithelial cell morphogenesis and formation of branching tubules (By similarity).
See full target information TIMELESS
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Publications (2)

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Sleep & breathing = Schlaf & Atmung 28:697-705 PubMed37923872

2023

Relationship between circadian rhythm-related genes and extracellular matrix: implications for sleep deprivation.

Applications

Unspecified application

Species

Unspecified reactive species

Xuejuan Liu,Jia Sun,Zexia Ling,Tong Dong

The EMBO journal 42:e113475 PubMed37357575

2023

Cohesin-mediated DNA loop extrusion resolves sister chromatids in G2 phase.

Applications

Unspecified application

Species

Unspecified reactive species

Paul Batty,Christoph Ch Langer,Zsuzsanna Takács,Wen Tang,Claudia Blaukopf,Jan-Michael Peters,Daniel W Gerlich
View all publications
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