AB248557

Anti-Timeless antibody [EPR5274(2)] - BSA and Azide free

RabMAb
Recombinant
What is this?

Be the first to review this product! Submit a review

(1 Publication)

Rabbit Recombinant Monoclonal Timeless antibody. Carrier free. Suitable for WB, Flow Cyt (Intra) and reacts with Human, Rat, Mouse samples. Cited in 1 publication.

View Alternative Names

TIM, TIM1, TIMELESS1, TIMELESS, Protein timeless homolog, hTIM

2 Images

Flow Cytometry (Intracellular) - Anti-Timeless antibody [EPR5274(2)] - BSA and Azide free (AB248557)

Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-Timeless antibody [EPR5274(2)] - BSA and Azide free (AB248557)

This data was developed using ab133569, the same antibody clone in a different buffer formulation.

Intracellular Flow Cytometry analysis of permeabilized mouse NIH/3T3 cells staining Timeless using ab133569 at a 1/10 dilution (red) or a rabbit IgG control antibody (green).

Unknown

Western blot - Anti-Timeless antibody [EPR5274(2)] - BSA and Azide free (AB248557)

This data was developed using ab133569, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-Timeless antibody [EPR5274(2)] (<a href='/en-us/products/primary-antibodies/timeless-antibody-epr52742-ab133569'>ab133569</a>) at 1/1000 dilution

Lane 1:

Jurkat cell lysate at 10 µg

Lane 2:

HeLa cell lysate at 10 µg

Lane 3:

JAR cell lysate at 10 µg

Lane 4:

RAW 264.7 cell lysate at 10 µg

Lane 5:

PC12 cell lysate at 10 µg

Lane 6:

NIH/3T3 cell lysate at 10 µg

Secondary

All lanes:

Standard HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 139 kDa

true

Unconjugated

Anti-Timeless antibody [EPR5274(2)]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR5274(2)

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse, Rat, Human

Applications

WB, Flow Cyt (Intra)

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style

left-column

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Mouse": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }

style

left-column

Product details

ab248557 is the carrier-free version of ab133569.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

style

left-column

Properties and storage information

Form

Liquid

Purification technique

Affinity purification

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Timeless also known as timeless circadian regulator is a protein with a molecular mass of about 139 kDa. It plays an important role in the stabilization of the circadian rhythm in mammals. Timeless associates with other clock proteins and participates in the feedback loop that drives circadian oscillations influencing hormonal and metabolic pathways. Researchers find Timeless expression primarily in the suprachiasmatic nucleus of the brain the central circadian pacemaker but it is also present in peripheral tissues.

Biological function summary

The protein influences the circadian rhythm by interacting with CLOCK and BMAL1 forming part of a macromolecular complex. Timeless helps sustain the proper timing and expression of various genes influenced by the circadian rhythm ensuring an orderly and synchronized cellular process across tissues. Its interaction with other clock components regulates circadian-controlled pathways governing processes like sleep-wake cycles hormone release and cell metabolism.

Pathways

Timeless participates in the regulation of the circadian rhythm and the cell cycle. It interacts with PER1 and PER2 proteins central to the inhibition of the CLOCK/BMAL1 complex which plays a pivotal role in maintaining the circadian clock's precision. Timeless also interfaces with proteins involved in DNA damage response pathways promoting effective DNA repair during cell division and aligning cell cycle regulation with circadian rhythms.

Timeless has been linked to cancer and sleep disorders. The protein's role in DNA repair and cell cycle control associates it with tumorigenesis when its function becomes dysregulated. Additionally disruptions in Timeless expression or function are connected to sleep phase disorders highlighting its involvement in maintaining proper circadian function. In cancer alterations in Timeless could lead to interactions with oncogenes or tumor suppressors potentially affecting cancer progression.

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

Plays an important role in the control of DNA replication, maintenance of replication fork stability, maintenance of genome stability throughout normal DNA replication, DNA repair and in the regulation of the circadian clock (PubMed : 17141802, PubMed : 17296725, PubMed : 23359676, PubMed : 23418588, PubMed : 26344098, PubMed : 31138685, PubMed : 32705708, PubMed : 35585232, PubMed : 9856465). Required to stabilize replication forks during DNA replication by forming a complex with TIPIN : this complex regulates DNA replication processes under both normal and stress conditions, stabilizes replication forks and influences both CHEK1 phosphorylation and the intra-S phase checkpoint in response to genotoxic stress (PubMed : 17141802, PubMed : 17296725, PubMed : 23359676, PubMed : 35585232). During DNA replication, inhibits the CMG complex ATPase activity and activates DNA polymerases catalytic activities, coupling DNA unwinding and DNA synthesis (PubMed : 23359676). TIMELESS promotes TIPIN nuclear localization (PubMed : 17141802, PubMed : 17296725). Plays a role in maintaining processive DNA replication past genomic guanine-rich DNA sequences that form G-quadruplex (G4) structures, possibly together with DDX11 (PubMed : 32705708). Involved in cell survival after DNA damage or replication stress by promoting DNA repair (PubMed : 17141802, PubMed : 17296725, PubMed : 26344098, PubMed : 30356214). In response to double-strand breaks (DSBs), accumulates at DNA damage sites and promotes homologous recombination repair via its interaction with PARP1 (PubMed : 26344098, PubMed : 30356214, PubMed : 31138685). May be specifically required for the ATR-CHEK1 pathway in the replication checkpoint induced by hydroxyurea or ultraviolet light (PubMed : 15798197). Involved in the determination of period length and in the DNA damage-dependent phase advancing of the circadian clock (PubMed : 23418588, PubMed : 31138685). Negatively regulates CLOCK|NPAS2-ARTNL/BMAL1|ARTNL2/BMAL2-induced transactivation of PER1 possibly via translocation of PER1 into the nucleus (PubMed : 31138685, PubMed : 9856465). May play a role as destabilizer of the PER2-CRY2 complex (PubMed : 31138685). May also play an important role in epithelial cell morphogenesis and formation of branching tubules (By similarity).

See full target information TIMELESS

style

left-column

Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Psychiatry investigation 22:699-713 PubMed40566894

2025

Transcriptional Landscape and Biomarker Discovery for Endoplasmic Reticulum Stress in Alzheimer's Disease: An Ex Vivo Study Using Patients-Derived Dermal Fibroblasts.

Applications

Unspecified application

Species

Unspecified reactive species

Yeojin Kim,You Jin Nam,Sunwoo Yoon,Young Joon Cho,Ho Min Song,Seongmin Kim,Donghyuk Shin,Jin Young Noh,Sun Min Lee,So Young Moon,Eun-Joo Kim,Soo Hyun Cho,Byeong C Kim,Seong Hye Choi,Sang Won Seo,Jin Wook Choi,Young-Sil An,Bumhee Park,Young Joon Park,Hee Young Kang,Hyun Goo Woo,Yong Hyuk Cho,Sunhwa Hong,Sang Joon Son,Sang-Rae Lee,Chang Hyung Hong,Hyun Woong Roh

View all publications

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Timeless antibody [EPR5274(2)] - BSA and Azide free

Flow Cytometry (Intracellular) - Anti-Timeless antibody [EPR5274(2)] - BSA and Azide free (AB248557)

Western blot - Anti-Timeless antibody [EPR5274(2)] - BSA and Azide free (AB248557)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

Product promise