JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB239982

Anti-Timeless antibody [EPR5275] - BSA and Azide free

Be the first to review this product! Submit a review

|

(1 Publication)

Rabbit Recombinant Monoclonal Timeless antibody. Carrier free. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 1 publication.

View Alternative Names

TIM, TIM1, TIMELESS1, TIMELESS, Protein timeless homolog, hTIM

3 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Timeless antibody [EPR5275] - BSA and Azide free (AB239982)
  • IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Timeless antibody [EPR5275] - BSA and Azide free (AB239982)

This data was developed using ab109512, the same antibody clone in a different buffer formulation.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human lung cancer tissue sections labeling Timeless with purified ab109512 at 1/500 dilution (0.25 μg/mL). Heat mediated antigen retrieval was performed using Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 1 (pH 6.0). Tissue was counterstained with Hematoxylin. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) secondary antibody was used. PBS instead of the primary antibody was used as the negative control.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

Immunocytochemistry/ Immunofluorescence - Anti-Timeless antibody [EPR5275] - BSA and Azide free (AB239982)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Timeless antibody [EPR5275] - BSA and Azide free (AB239982)

This data was developed using ab109512, the same antibody clone in a different buffer formulation.
Immunocytochemistry analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling Timeless with purified ab109512 at 1/50 dilution (2.5 μg/mL). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.5 μg/mL). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 (2 μg/mL) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

Western blot - Anti-Timeless antibody [EPR5275] - BSA and Azide free (AB239982)
  • WB

Unknown

Western blot - Anti-Timeless antibody [EPR5275] - BSA and Azide free (AB239982)

All lanes:

Western blot - Anti-Timeless antibody [EPR5275] (<a href='/en-us/products/primary-antibodies/timeless-antibody-epr5275-ab109512'>ab109512</a>) at 1/50000 dilution

Lane 1:

HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate

Lane 2:

JAR (Human placenta choriocarcinoma epithelial cell) whole cell lysate

Lane 3:

Jurkat (Human T cell leukemia T lymphocyte) whole cell lysate

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 139 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR5275

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

ICC/IF, IHC-P, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style
left-column

Alternative Products

Primary Antibodies

AB229218

Anti-Timeless antibody

Immunocytochemistry/ Immunofluorescence - Anti-Timeless antibody (AB229218)

  • 0
  • 0
Primary Antibodies

AB72458

Anti-Timeless antibody

BOND RX™ Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Timeless antibody (AB72458)

  • 5
  • 1
Primary Antibodies

AB133569

Anti-Timeless antibody [EPR5274(2)]

RabMAb|Recombinant

Flow Cytometry (Intracellular) - Anti-Timeless antibody [EPR5274(2)] (AB133569)

  • 5
  • 1

Complementary Products

Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Primary Antibodies

AB9485

Anti-GAPDH antibody - Loading Control

BOND RX™ Validated|Lab Essentials

Immunocytochemistry/ Immunofluorescence - Anti-GAPDH antibody - Loading Control (AB9485)

  • 5
  • 88
Primary Antibodies

AB15580

Anti-Ki67 antibody

BOND RX™ Validated|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ki67 antibody (AB15580)

  • 5
  • 230
Primary Antibodies

AB6326

Anti-BrdU antibody [BU1/75 (ICR1)] - Proliferation Marker

Flow Cytometry (Intracellular) - Anti-BrdU antibody [BU1/75 (ICR1)] - Proliferation Marker (AB6326)

  • 5
  • 104
style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" } } }
style
left-column

Product details

ab239982 is the carrier-free version of ab109512.

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Timeless also known as timeless circadian regulator is a protein with a molecular mass of about 139 kDa. It plays an important role in the stabilization of the circadian rhythm in mammals. Timeless associates with other clock proteins and participates in the feedback loop that drives circadian oscillations influencing hormonal and metabolic pathways. Researchers find Timeless expression primarily in the suprachiasmatic nucleus of the brain the central circadian pacemaker but it is also present in peripheral tissues.
Biological function summary

The protein influences the circadian rhythm by interacting with CLOCK and BMAL1 forming part of a macromolecular complex. Timeless helps sustain the proper timing and expression of various genes influenced by the circadian rhythm ensuring an orderly and synchronized cellular process across tissues. Its interaction with other clock components regulates circadian-controlled pathways governing processes like sleep-wake cycles hormone release and cell metabolism.

Pathways

Timeless participates in the regulation of the circadian rhythm and the cell cycle. It interacts with PER1 and PER2 proteins central to the inhibition of the CLOCK/BMAL1 complex which plays a pivotal role in maintaining the circadian clock's precision. Timeless also interfaces with proteins involved in DNA damage response pathways promoting effective DNA repair during cell division and aligning cell cycle regulation with circadian rhythms.

Timeless has been linked to cancer and sleep disorders. The protein's role in DNA repair and cell cycle control associates it with tumorigenesis when its function becomes dysregulated. Additionally disruptions in Timeless expression or function are connected to sleep phase disorders highlighting its involvement in maintaining proper circadian function. In cancer alterations in Timeless could lead to interactions with oncogenes or tumor suppressors potentially affecting cancer progression.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Plays an important role in the control of DNA replication, maintenance of replication fork stability, maintenance of genome stability throughout normal DNA replication, DNA repair and in the regulation of the circadian clock (PubMed : 17141802, PubMed : 17296725, PubMed : 23359676, PubMed : 23418588, PubMed : 26344098, PubMed : 31138685, PubMed : 32705708, PubMed : 35585232, PubMed : 9856465). Required to stabilize replication forks during DNA replication by forming a complex with TIPIN : this complex regulates DNA replication processes under both normal and stress conditions, stabilizes replication forks and influences both CHEK1 phosphorylation and the intra-S phase checkpoint in response to genotoxic stress (PubMed : 17141802, PubMed : 17296725, PubMed : 23359676, PubMed : 35585232). During DNA replication, inhibits the CMG complex ATPase activity and activates DNA polymerases catalytic activities, coupling DNA unwinding and DNA synthesis (PubMed : 23359676). TIMELESS promotes TIPIN nuclear localization (PubMed : 17141802, PubMed : 17296725). Plays a role in maintaining processive DNA replication past genomic guanine-rich DNA sequences that form G-quadruplex (G4) structures, possibly together with DDX11 (PubMed : 32705708). Involved in cell survival after DNA damage or replication stress by promoting DNA repair (PubMed : 17141802, PubMed : 17296725, PubMed : 26344098, PubMed : 30356214). In response to double-strand breaks (DSBs), accumulates at DNA damage sites and promotes homologous recombination repair via its interaction with PARP1 (PubMed : 26344098, PubMed : 30356214, PubMed : 31138685). May be specifically required for the ATR-CHEK1 pathway in the replication checkpoint induced by hydroxyurea or ultraviolet light (PubMed : 15798197). Involved in the determination of period length and in the DNA damage-dependent phase advancing of the circadian clock (PubMed : 23418588, PubMed : 31138685). Negatively regulates CLOCK|NPAS2-ARTNL/BMAL1|ARTNL2/BMAL2-induced transactivation of PER1 possibly via translocation of PER1 into the nucleus (PubMed : 31138685, PubMed : 9856465). May play a role as destabilizer of the PER2-CRY2 complex (PubMed : 31138685). May also play an important role in epithelial cell morphogenesis and formation of branching tubules (By similarity).
See full target information TIMELESS
style
left-column

Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Alzheimer's research & therapy 17:47 PubMed39972387

2025

Cornuside alleviates cognitive impairments induced by Aβ through attenuating NLRP3-mediated neurotoxicity by promoting mitophagy.

Applications

Unspecified application

Species

Unspecified reactive species

Fulin Zhou,Wenwen Lian,Xiaotang Yuan,Zexing Wang,Congyuan Xia,Yu Yan,Wenping Wang,Zhuohang Tong,Yungchi Cheng,Jiekun Xu,Jun He,Weiku Zhang
View all publications
style
left-column
style
left-column
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com