AB192246

Anti-TIMM17A/TIM17 antibody [EPR16098]

Name: Anti-TIMM17A/TIM17 antibody [EPR16098] (ab192246) | Abcam
Brand: Abcam Limited
SKU: AB192246
Price: 585 USD
Availability: InStock
Rating: 3 (5 reviews)

RabMAb
Recombinant
What is this?

(5 Reviews)

(6 Publications)

Rabbit Recombinant Monoclonal TIMM17A/TIM17 antibody. Suitable for IP, WB, ICC/IF and reacts with Human, Mouse, Rat samples. Cited in 6 publications.

View Alternative Names

MIMT17, TIM17, TIM17A, TIMM17, TIMM17A, Mitochondrial import inner membrane translocase subunit Tim17-A, Inner membrane preprotein translocase Tim17a

4 Images

Immunocytochemistry/ Immunofluorescence - Anti-TIMM17A/TIM17 antibody [EPR16098] (AB192246)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-TIMM17A/TIM17 antibody [EPR16098] (AB192246)

Immunofluorescent analysis of 4% paraformaldehyde-fixed 0.1% tritonX-100 permeabilized HeLa cells labeling TIMM17A/TIM17 with ab192246 at 1/50 dilution (11.7 μg/ml) followed by Goat anti rabbit IgG (Alexa Fluor® 555) secondary antibody (ab150078) at 1/500 dilution. Nuclear counter stain Dapi (blue).

The two negative controls are ab192246 at 1/50 dilution followed by (Goat anti mouse IgG (Alexa Fluor®488) secondary antibody at 1/200 dilution.

Supplier Data

Immunoprecipitation - Anti-TIMM17A/TIM17 antibody [EPR16098] (AB192246)

Western blot analysis of TIMM17A/TIM17 in HeLa cell lysates immunoprecipitated using ab192246 at 1/40 dilution (Lane 1). Lane 2 : PBS instead of HeLa lysate.

Secondary antibody : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 dilution.

Blocking/Dilution buffer : 5% NFDM /TBST.

All lanes:

Immunoprecipitation - Anti-TIMM17A/TIM17 antibody [EPR16098] (ab192246)

Predicted band size: 18 kDa

false

Supplier Data

Western blot - Anti-TIMM17A/TIM17 antibody [EPR16098] (AB192246)

Blocking/Dilution buffer : 5% NFDM /TBST.

All lanes:

Western blot - Anti-TIMM17A/TIM17 antibody [EPR16098] (ab192246) at 1/1000 dilution

Lane 1:

HeLa cell lysate at 20 µg

Lane 2:

K562 cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 18 kDa

Observed band size: 17 kDa

false

Supplier Data

Western blot - Anti-TIMM17A/TIM17 antibody [EPR16098] (AB192246)

Blocking/Dilution buffer : 5% NFDM /TBST.

All lanes:

Western blot - Anti-TIMM17A/TIM17 antibody [EPR16098] (ab192246) at 1/1000 dilution

Lane 1:

RAW 264.7 cell lysate at 10 µg

Lane 2:

PC-12 cell lysate at 10 µg

Lane 3:

NIH/3T3 cell lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 18 kDa

Observed band size: 17 kDa

false

Carrier free

Anti-TIMM17A/TIM17 antibody [EPR16098] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR16098

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

ICC/IF, IP, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/40", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50", "ICCIF-species-notes": "" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

TIMM17A also known as TIM17A or simply TIM17 is a protein component of the mitochondrial inner membrane translocase complex. It is involved in the import of nuclear-encoded proteins into the mitochondria. With a molecular mass of approximately 19 kDa TIMM17A is primarily expressed in the inner mitochondrial membrane. This protein forms part of the TIM23 complex which mediates the translocation of presequence-containing preproteins into the mitochondrial inner membrane or matrix.

Biological function summary

The TIMM17A protein facilitates the import of essential mitochondrial proteins securing proper mitochondrial function and energy production. TIMM17A as part of the TIM23 translocase complex cooperates closely with other TIM proteins such as TIMM50 and TIMM23 to ensure protein import is efficient and targeted. Its role in maintaining mitochondrial integrity also supports cellular metabolism and the regulation of apoptotic processes which are processes responsible for programmed cell death.

Pathways

The TIMM17A protein plays a significant role in the mitochondrial protein import pathway. It interacts with other key proteins like HSP70 and mtHSP70 which manipulate peptide folding and assist in pulling preproteins through the TIM23 channel. The proper import of these proteins is essential for the oxidative phosphorylation pathway which is important for ATP production. This pathway enables ATP synthesis by transferring electrons through a sequence of electron transport chain complexes located in the mitochondrial inner membrane.

Research associates TIMM17A dysfunction with mitochondrial myopathy a disorder characterized by muscle weakness and dysfunction due to impaired mitochondrial performance. Studies also link alterations in TIMM17A to certain neurodegenerative disorders such as Parkinson’s disease where mitochondrial dysfunction plays an important role. The protein's interaction with other import machinery members during these conditions like TIMM44 highlights its importance in maintaining mitochondrial health and function.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Essential component of the TIM23 complex, a complex that mediates the translocation of transit peptide-containing proteins across the mitochondrial inner membrane.

See full target information TIMM17A

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Publications (6)

Recent publications for all applications. Explore the full list and refine your search

Cell reports 43:115038 PubMed39630581

2024

OCIAD1 and prohibitins regulate the stability of the TIM23 protein translocase.

Applications

Unspecified application

Species

Unspecified reactive species

Praveenraj Elancheliyan,Klaudia K Maruszczak,Remigiusz Adam Serwa,Till Stephan,Ahmet Sadik Gulgec,Mayra A Borrero-Landazabal,Sonia Ngati,Aleksandra Gosk,Stefan Jakobs,Michal Wasilewski,Agnieszka Chacinska

Life (Basel, Switzerland) 13: PubMed37240849

2023

Aerobic Exercise Delays Alzheimer's Disease by Regulating Mitochondrial Proteostasis in the Cerebral Cortex and Hippocampus.

Applications

Unspecified application

Species

Unspecified reactive species

Kaiyin Cui,Chaoyang Li,Guoliang Fang

Antioxidants (Basel, Switzerland) 12: PubMed36670904

2022

Fundamental Role of Pentose Phosphate Pathway within the Endoplasmic Reticulum in Glutamine Addiction of Triple-Negative Breast Cancer Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Cecilia Marini,Vanessa Cossu,Sonia Carta,Elisa Greotti,Daniela Gaglio,Nadia Bertola,Sabrina Chiesa,Silvia Bruno,Francesca Vitale,Marcella Bonanomi,Danilo Porro,Mattia Riondato,Anna Maria Orengo,Matteo Bauckneht,Silvia Morbelli,Silvia Ravera,Gianmario Sambuceti

Frontiers in physiology 12:718982 PubMed34912235

2021

Neurogenic Hypertension Mediated Mitochondrial Abnormality Leads to Cardiomyopathy: Contribution of UPR and Norepinephrine-miR- 18a-5p-HIF-1α Axis.

Applications

Unspecified application

Species

Unspecified reactive species

Shyam S Nandi,Kenichi Katsurada,Sushil K Mahata,Kaushik P Patel

Scientific reports 11:18916 PubMed34556702

2021

Neuronal mitochondrial dysfunction in sporadic amyotrophic lateral sclerosis is developmentally regulated.

Applications

Unspecified application

Species

Unspecified reactive species

Tanisha Singh,Yuanyuan Jiao,Lisa M Ferrando,Svitlana Yablonska,Fang Li,Emily C Horoszko,David Lacomis,Robert M Friedlander,Diane L Carlisle

EMBO reports 21:e48833 PubMed31721420

2019

MITRAC15/COA1 promotes mitochondrial translation in a ND2 ribosome-nascent chain complex.

Applications

Unspecified application

Species

Unspecified reactive species

Cong Wang,Ricarda Richter-Dennerlein,David Pacheu-Grau,Fan Liu,Ying Zhu,Sven Dennerlein,Peter Rehling

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-TIMM17A/TIM17 antibody [EPR16098]

Immunocytochemistry/ Immunofluorescence - Anti-TIMM17A/TIM17 antibody [EPR16098] (AB192246)

Immunoprecipitation - Anti-TIMM17A/TIM17 antibody [EPR16098] (AB192246)

Western blot - Anti-TIMM17A/TIM17 antibody [EPR16098] (AB192246)

Western blot - Anti-TIMM17A/TIM17 antibody [EPR16098] (AB192246)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (6)

Product promise