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Rabbit Recombinant Monoclonal TIMM17A/TIM17 antibody. Carrier free. Suitable for ICC/IF, IP, WB and reacts with Human, Mouse, Rat samples. Cited in 1 publication.

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Images

Western blot - Anti-TIMM17A/TIM17 antibody [EPR16098] - BSA and Azide free (AB251117), expandable thumbnail
  • Western blot - Anti-TIMM17A/TIM17 antibody [EPR16098] - BSA and Azide free (AB251117), expandable thumbnail
  • Immunoprecipitation - Anti-TIMM17A/TIM17 antibody [EPR16098] - BSA and Azide free (AB251117), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-TIMM17A/TIM17 antibody [EPR16098] - BSA and Azide free (AB251117), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Constituents: PBS

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
ICC/IFIPWB
Human
Tested
Tested
Tested
Mouse
Expected
Expected
Tested
Rat
Expected
Expected
Tested

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Expected
Expected

Species
Mouse, Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Expected
Expected

Species
Mouse, Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Mouse, Rat, Human
Dilution info
-
Notes

-

Target data

Function

Essential component of the TIM23 complex, a complex that mediates the translocation of transit peptide-containing proteins across the mitochondrial inner membrane.

Alternative names

MIMT17, TIM17, TIM17A, TIMM17, TIMM17A, Mitochondrial import inner membrane translocase subunit Tim17-A, Inner membrane preprotein translocase Tim17a

Recommended products

Rabbit Recombinant Monoclonal TIMM17A/TIM17 antibody. Carrier free. Suitable for ICC/IF, IP, WB and reacts with Human, Mouse, Rat samples. Cited in 1 publication.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free
Yes
Clone number
EPR16098
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze

Notes

ab251117 is the carrier-free version of Anti-TIMM17A/TIM17 antibody [EPR16098] ab192246.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

TIMM17A also known as TIM17A or simply TIM17 is a protein component of the mitochondrial inner membrane translocase complex. It is involved in the import of nuclear-encoded proteins into the mitochondria. With a molecular mass of approximately 19 kDa TIMM17A is primarily expressed in the inner mitochondrial membrane. This protein forms part of the TIM23 complex which mediates the translocation of presequence-containing preproteins into the mitochondrial inner membrane or matrix.

Biological function summary

The TIMM17A protein facilitates the import of essential mitochondrial proteins securing proper mitochondrial function and energy production. TIMM17A as part of the TIM23 translocase complex cooperates closely with other TIM proteins such as TIMM50 and TIMM23 to ensure protein import is efficient and targeted. Its role in maintaining mitochondrial integrity also supports cellular metabolism and the regulation of apoptotic processes which are processes responsible for programmed cell death.

Pathways

The TIMM17A protein plays a significant role in the mitochondrial protein import pathway. It interacts with other key proteins like HSP70 and mtHSP70 which manipulate peptide folding and assist in pulling preproteins through the TIM23 channel. The proper import of these proteins is essential for the oxidative phosphorylation pathway which is important for ATP production. This pathway enables ATP synthesis by transferring electrons through a sequence of electron transport chain complexes located in the mitochondrial inner membrane.

Associated diseases and disorders

Research associates TIMM17A dysfunction with mitochondrial myopathy a disorder characterized by muscle weakness and dysfunction due to impaired mitochondrial performance. Studies also link alterations in TIMM17A to certain neurodegenerative disorders such as Parkinson’s disease where mitochondrial dysfunction plays an important role. The protein's interaction with other import machinery members during these conditions like TIMM44 highlights its importance in maintaining mitochondrial health and function.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

4 product images

  • Western blot - Anti-TIMM17A/TIM17 antibody [EPR16098] - BSA and Azide free (ab251117), expandable thumbnail

    Western blot - Anti-TIMM17A/TIM17 antibody [EPR16098] - BSA and Azide free (ab251117)

    This data was developed using Anti-TIMM17A/TIM17 antibody [EPR16098] ab192246, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

    All lanes: Western blot - Anti-TIMM17A/TIM17 antibody [EPR16098] (Anti-TIMM17A/TIM17 antibody [EPR16098] ab192246) at 1/1000 dilution

    Lane 1: HeLa cell lysate at 20 µg

    Lane 2: K562 cell lysate at 20 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 18 kDa

    Observed band size: 17 kDa

  • Western blot - Anti-TIMM17A/TIM17 antibody [EPR16098] - BSA and Azide free (ab251117), expandable thumbnail

    Western blot - Anti-TIMM17A/TIM17 antibody [EPR16098] - BSA and Azide free (ab251117)

    This data was developed using Anti-TIMM17A/TIM17 antibody [EPR16098] ab192246, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

    All lanes: Western blot - Anti-TIMM17A/TIM17 antibody [EPR16098] (Anti-TIMM17A/TIM17 antibody [EPR16098] ab192246) at 1/1000 dilution

    Lane 1: RAW 264.7 cell lysate at 10 µg

    Lane 2: PC-12 cell lysate at 10 µg

    Lane 3: NIH/3T3 cell lysate at 10 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 18 kDa

    Observed band size: 17 kDa

  • Immunoprecipitation - Anti-TIMM17A/TIM17 antibody [EPR16098] - BSA and Azide free (ab251117), expandable thumbnail

    Immunoprecipitation - Anti-TIMM17A/TIM17 antibody [EPR16098] - BSA and Azide free (ab251117)

    This data was developed using Anti-TIMM17A/TIM17 antibody [EPR16098] ab192246, the same antibody clone in a different buffer formulation.Western blot analysis of TIMM17A/TIM17 in HeLa cell lysates immunoprecipitated using Anti-TIMM17A/TIM17 antibody [EPR16098] ab192246 at 1/40 dilution (Lane 1). Lane 2: PBS instead of HeLa lysate. Secondary antibody: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 dilution. Blocking/Dilution buffer: 5% NFDM /TBST.

    All lanes: Immunoprecipitation - Anti-TIMM17A/TIM17 antibody [EPR16098] (Anti-TIMM17A/TIM17 antibody [EPR16098] ab192246)

    Predicted band size: 18 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-TIMM17A/TIM17 antibody [EPR16098] - BSA and Azide free (ab251117), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-TIMM17A/TIM17 antibody [EPR16098] - BSA and Azide free (ab251117)

    This data was developed using Anti-TIMM17A/TIM17 antibody [EPR16098] ab192246, the same antibody clone in a different buffer formulation.Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% tritonX-100 permeabilized HeLa cells labeling TIMM17A/TIM17 with Anti-TIMM17A/TIM17 antibody [EPR16098] ab192246 at 1/50 dilution (11.7 μg/ml), followed by Goat anti rabbit IgG (Alexa Fluor® 555) secondary antibody (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 555) ab150078) at 1/500 dilution. Nuclear counter stain Dapi (blue). The two negative controls are Anti-TIMM17A/TIM17 antibody [EPR16098] ab192246 at 1/50 dilution followed by (Goat anti mouse IgG (Alexa Fluor®488) secondary antibody at 1/200 dilution.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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