AB251117

Anti-TIMM17A/TIM17 antibody [EPR16098] - BSA and Azide free

RabMAb
Recombinant
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(2 Publications)

Rabbit Recombinant Monoclonal TIMM17A/TIM17 antibody. Carrier free. Suitable for ICC/IF, IP, WB and reacts with Human, Mouse, Rat samples. Cited in 2 publications.

View Alternative Names

MIMT17, TIM17, TIM17A, TIMM17, TIMM17A, Mitochondrial import inner membrane translocase subunit Tim17-A, Inner membrane preprotein translocase Tim17a

4 Images

Immunocytochemistry/ Immunofluorescence - Anti-TIMM17A/TIM17 antibody [EPR16098] - BSA and Azide free (AB251117)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-TIMM17A/TIM17 antibody [EPR16098] - BSA and Azide free (AB251117)

This data was developed using ab192246, the same antibody clone in a different buffer formulation.

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% tritonX-100 permeabilized HeLa cells labeling TIMM17A/TIM17 with ab192246 at 1/50 dilution (11.7 μg/ml), followed by Goat anti rabbit IgG (Alexa Fluor® 555) secondary antibody (ab150078) at 1/500 dilution. Nuclear counter stain Dapi (blue).

The two negative controls are ab192246 at 1/50 dilution followed by (Goat anti mouse IgG (Alexa Fluor®488) secondary antibody at 1/200 dilution.

Supplier Data

Immunoprecipitation - Anti-TIMM17A/TIM17 antibody [EPR16098] - BSA and Azide free (AB251117)

This data was developed using ab192246, the same antibody clone in a different buffer formulation.

Western blot analysis of TIMM17A/TIM17 in HeLa cell lysates immunoprecipitated using ab192246 at 1/40 dilution (Lane 1). Lane 2 : PBS instead of HeLa lysate.

Secondary antibody : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 dilution.

Blocking/Dilution buffer : 5% NFDM /TBST.

All lanes:

Immunoprecipitation - Anti-TIMM17A/TIM17 antibody [EPR16098] (<a href='/en-us/products/primary-antibodies/timm17a-tim17-antibody-epr16098-ab192246'>ab192246</a>)

Predicted band size: 18 kDa

false

Supplier Data

Western blot - Anti-TIMM17A/TIM17 antibody [EPR16098] - BSA and Azide free (AB251117)

This data was developed using ab192246, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-TIMM17A/TIM17 antibody [EPR16098] (<a href='/en-us/products/primary-antibodies/timm17a-tim17-antibody-epr16098-ab192246'>ab192246</a>) at 1/1000 dilution

Lane 1:

HeLa cell lysate at 20 µg

Lane 2:

K562 cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 18 kDa

Observed band size: 17 kDa

false

Supplier Data

Western blot - Anti-TIMM17A/TIM17 antibody [EPR16098] - BSA and Azide free (AB251117)

This data was developed using ab192246, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-TIMM17A/TIM17 antibody [EPR16098] (<a href='/en-us/products/primary-antibodies/timm17a-tim17-antibody-epr16098-ab192246'>ab192246</a>) at 1/1000 dilution

Lane 1:

RAW 264.7 cell lysate at 10 µg

Lane 2:

PC-12 cell lysate at 10 µg

Lane 3:

NIH/3T3 cell lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 18 kDa

Observed band size: 17 kDa

false

Unconjugated

Anti-TIMM17A/TIM17 antibody [EPR16098]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR16098

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse, Rat, Human

Applications

WB, ICC/IF, IP

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Mouse": { "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Product details

ab251117 is the carrier-free version of ab192246.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form

Liquid

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

TIMM17A also known as TIM17A or simply TIM17 is a protein component of the mitochondrial inner membrane translocase complex. It is involved in the import of nuclear-encoded proteins into the mitochondria. With a molecular mass of approximately 19 kDa TIMM17A is primarily expressed in the inner mitochondrial membrane. This protein forms part of the TIM23 complex which mediates the translocation of presequence-containing preproteins into the mitochondrial inner membrane or matrix.

Biological function summary

The TIMM17A protein facilitates the import of essential mitochondrial proteins securing proper mitochondrial function and energy production. TIMM17A as part of the TIM23 translocase complex cooperates closely with other TIM proteins such as TIMM50 and TIMM23 to ensure protein import is efficient and targeted. Its role in maintaining mitochondrial integrity also supports cellular metabolism and the regulation of apoptotic processes which are processes responsible for programmed cell death.

Pathways

The TIMM17A protein plays a significant role in the mitochondrial protein import pathway. It interacts with other key proteins like HSP70 and mtHSP70 which manipulate peptide folding and assist in pulling preproteins through the TIM23 channel. The proper import of these proteins is essential for the oxidative phosphorylation pathway which is important for ATP production. This pathway enables ATP synthesis by transferring electrons through a sequence of electron transport chain complexes located in the mitochondrial inner membrane.

Research associates TIMM17A dysfunction with mitochondrial myopathy a disorder characterized by muscle weakness and dysfunction due to impaired mitochondrial performance. Studies also link alterations in TIMM17A to certain neurodegenerative disorders such as Parkinson’s disease where mitochondrial dysfunction plays an important role. The protein's interaction with other import machinery members during these conditions like TIMM44 highlights its importance in maintaining mitochondrial health and function.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Essential component of the TIM23 complex, a complex that mediates the translocation of transit peptide-containing proteins across the mitochondrial inner membrane.

See full target information TIMM17A

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Cell death & disease 14:760 PubMed37993451

2023

The BRD4-SRPK2-SRSF2 signal modulates the splicing efficiency of ACSL3 pre-mRNA and influences erastin-induced ferroptosis in osteosarcoma cells.

Applications

Unspecified application

Species

Unspecified reactive species

Shun-Hong Luo,Jia-Ming Tian,Yi Chu,Hong-Yi Zhu,Jiang-Dong Ni,Jun Huang

International journal of molecular sciences 22: PubMed34769092

2021

11,12 Epoxyeicosatrienoic Acid Rescues Deteriorated Wound Healing in Diabetes.

Applications

Unspecified application

Species

Unspecified reactive species

Katharina Sommer,Heike Jakob,Caroline Reiche,Dirk Henrich,Jasmina Sterz,Johannes Frank,Ingo Marzi,Anna Lena Sander

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-TIMM17A/TIM17 antibody [EPR16098] - BSA and Azide free

Immunocytochemistry/ Immunofluorescence - Anti-TIMM17A/TIM17 antibody [EPR16098] - BSA and Azide free (AB251117)

Immunoprecipitation - Anti-TIMM17A/TIM17 antibody [EPR16098] - BSA and Azide free (AB251117)

Western blot - Anti-TIMM17A/TIM17 antibody [EPR16098] - BSA and Azide free (AB251117)

Western blot - Anti-TIMM17A/TIM17 antibody [EPR16098] - BSA and Azide free (AB251117)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

Product promise