AB230253

Anti-TIMM23/TIM23 antibody

Name: Anti-TIMM23/TIM23 antibody (ab230253) | Abcam
Brand: Abcam Limited
SKU: AB230253
Price: 390 USD
Availability: InStock
Rating: 4 (4 reviews)

(4 Reviews)

(18 Publications)

Rabbit Polyclonal TIMM23/TIM23 antibody. Suitable for WB, IHC-P and reacts with Mouse, Human samples. Cited in 18 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human TIMM23.

View Alternative Names

TIM23, TIMM23, Mitochondrial import inner membrane translocase subunit Tim23

3 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TIMM23/TIM23 antibody (AB230253)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TIMM23/TIM23 antibody (AB230253)

Paraffin-embedded human testis tissue stained for TIMM23/TIM23 with ab230253 at 1/100 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TIMM23/TIM23 antibody (AB230253)

Paraffin-embedded human ovarian cancer tissue stained for TIMM23/TIM23 with ab230253 at 1/100 dilution in immunohistochemical analysis.

Supplier Data

Western blot - Anti-TIMM23/TIM23 antibody (AB230253)

All lanes:

Western blot - Anti-TIMM23/TIM23 antibody (ab230253) at 1/1000 dilution

Lane 1:

Mouse heart tissue lysate

Lane 2:

Mouse liver tissue lysate

Lane 3:

A431 (human epidermoid carcinoma cell line) whole cell lysate

Lane 4:

MCF7 (human breast adenocarcinoma cell line) whole cell lysate

Lane 5:

Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate

Secondary

All lanes:

Goat polyclonal to rabbit IgG at 1/10000 dilution

Predicted band size: 22 kDa

Observed band size: 22 kDa

true

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

WB, IHC-P

applications

Immunogen

Recombinant Full Length Protein corresponding to Human TIMM23.

O14925

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/20 - 1/200", "IHCP-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Orangutan": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.3 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

TIMM23 also known as TIM23 protein is a critical component of the mitochondrial import machinery. It weighs approximately 24 kDa and is expressed in the inner mitochondrial membrane. TIMM23 is part of the translocase of inner mitochondrial membrane (TIM) complex which is essential for translocating proteins from the mitochondrial intermembrane space into the matrix. The TIM23 complex facilitates the passage of pre-sequence or matrix-targeted proteins that have signal sequences recognizable by the mitochondrial import machinery.

Biological function summary

TIMM23 impacts mitochondrial function by cooperating with the TIM complex to ensure the proper import and processing of essential mitochondrial proteins. The complex known to include other subunits like TIMM17 and TIMM50 organizes the translocase activity required for maintaining mitochondrial homeostasis. TIMM23’s function is pivotal for energy production as it enables the importation of proteins important to several enzymatic processes particularly in oxidative phosphorylation.

Pathways

Proteins like TIMM23 play a role in maintaining mitochondrial integrity which is essential for metabolic pathways. In particular TIMM23 is integral to the oxidative phosphorylation pathway linking it with proteins such as ATP synthase involved in ATP production. The electron transport chain also depends indirectly on TIMM23 for ensuring that necessary proteins reach the inner mitochondrial membrane implicating TIMM23 in cellular respiration processes.

TIMM23 aberrations can result in mitochondrial dysfunction contributing to conditions such as mitochondrial myopathy. The disruption in protein import can affect energy metabolism which is a common feature in diseases like Leigh syndrome. Other proteins such as complex I and complex IV of the electron transport chain can also exhibit dysfunction when affected by a compromised TIMM23 function highlighting its role in maintaining cellular energy efficiency.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Essential component of the TIM23 complex, a complex that mediates the translocation of transit peptide-containing proteins across the mitochondrial inner membrane (PubMed : 10339406). Has a role in the activation of stress-induced mitophagy by protecting PINK1 from OMA1-mediated degradation and facilitating its accumulation at the outer mitochondrial membrane in response to depolarization (PubMed : 37160114).

See full target information TIMM23

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Publications (18)

Recent publications for all applications. Explore the full list and refine your search

Diagnostic pathology 20:79 PubMed40604870

2025

SHMT2 overexpression improves glaucoma by enhancing mitophagy in retinal ganglion cells through promoting the phospho of PINK1.

Applications

Unspecified application

Species

Unspecified reactive species

Liying Cui,Baojun Wang

Nature communications 16:5133 PubMed40461459

2025

ROMO1 overexpression protects the mitochondrial cysteinome from oxidations in aging.

Applications

Unspecified application

Species

Unspecified reactive species

Fengli Xu,Haipeng Huang,Kun Peng,Chongshu Jian,Hao Wu,Zhiwen Jing,Shan Qiu,Ying Chen,Keke Liu,Ling Fu,Yanru Wang,Jing Yang,Xiaotao Duan,Chu Wang,Heping Cheng,Xianhua Wang

Cell death & disease 15:491 PubMed38982043

2024

IL-27 disturbs lipid metabolism and restrains mitochondrial activity to inhibit γδ T17 cell-mediated skin inflammation.

Applications

Unspecified application

Species

Unspecified reactive species

Mingyue Zhang,Dehai Li,Jing Zhu,Xue Xia,Hua Zhang,Jie Wu,Shengli Wang,Anyi Deng,Qiong Wen,Jingyi Tan,Jianlei Hao,Jun Jiang,Xiucong Bao,Guodong Sun,Jiajing Lu,Quanli Yang,Hengwen Yang,Guangchao Cao,Zhinan Yin,Qian Wang

ACS synthetic biology 12:2498-2504 PubMed37506292

2023

A High-Throughput Colocalization Pipeline for Quantification of Mitochondrial Targeting across Different Protein Types.

Applications

Unspecified application

Species

Unspecified reactive species

Sierra K Lear,Jose A Nunez,Seth L Shipman

Cellular and molecular life sciences : CMLS 80:213 PubMed37464072

2023

DUSP1 interacts with and dephosphorylates VCP to improve mitochondrial quality control against endotoxemia-induced myocardial dysfunction.

Applications

Unspecified application

Species

Unspecified reactive species

Hang Zhu,Jin Wang,Ting Xin,Shanshan Chen,Ruiying Hu,Yukun Li,Mingming Zhang,Hao Zhou

Chembiochem : a European journal of chemical biology 24:e202200748 PubMed36946263

2023

Design and Synthesis of a Mitochondrial-Targeted JNK Inhibitor and Its Protective Effect on Parkinson's Disease Phenotypes.

Applications

Unspecified application

Species

Unspecified reactive species

Zhiqiang Zhou,Duoteng Zhang,Fangning Du,Weizhen An,Jingyan Ge,Changmin Yu,Naidi Yang,Chengwu Zhang,Kahleong Lim,Lin Li

Life (Basel, Switzerland) 13: PubMed37240849

2023

Aerobic Exercise Delays Alzheimer's Disease by Regulating Mitochondrial Proteostasis in the Cerebral Cortex and Hippocampus.

Applications

Unspecified application

Species

Unspecified reactive species

Kaiyin Cui,Chaoyang Li,Guoliang Fang

iScience 26:105745 PubMed36590171

2023

Centrosomal protein 120 promotes centrosome amplification and gastric cancer progression via USP54-mediated deubiquitination of PLK4.

Applications

Unspecified application

Species

Unspecified reactive species

Chenggang Zhang,Xianxiong Ma,Guanxin Wei,Xiuxian Zhu,Peng Hu,Xiang Chen,Dianshi Wang,Yuan Li,Tuo Ruan,Weikang Zhang,Kaixiong Tao,Chuanqing Wu

Cell death & disease 13:988 PubMed36418320

2022

COX7A1 enhances the sensitivity of human NSCLC cells to cystine deprivation-induced ferroptosis via regulating mitochondrial metabolism.

Applications

Unspecified application

Species

Unspecified reactive species

Yetong Feng,Jiayi Xu,Mengjiao Shi,Rongrong Liu,Lei Zhao,Xin Chen,Miaomiao Li,Yaping Zhao,Jiahui Chen,Wenjing Du,Pengfei Liu

Cellular & molecular biology letters 27:66 PubMed35945510

2022

SESN2 prevents the slow-to-fast myofiber shift in denervated atrophy via AMPK/PGC-1α pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaofan Yang,Pingping Xue,Zhenyu Liu,Wenqing Li,Chuyan Li,Zhenbing Chen

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-TIMM23/TIM23 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TIMM23/TIM23 antibody (AB230253)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TIMM23/TIM23 antibody (AB230253)

Western blot - Anti-TIMM23/TIM23 antibody (AB230253)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (18)

Product promise