Rabbit Polyclonal TIMM9 antibody. Suitable for IHC-P, ICC/IF and reacts with Human samples. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human TIMM9.
Images
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine)- Form
Liquid
- Clonality
Polyclonal
Immunogen
- Recombinant Full Length Protein corresponding to Human TIMM9. Database link Q9Y5J7
Reactivity data
Select an application| IHC-P | ICC/IF | |
|---|---|---|
| Human | Tested | Tested |
| Mouse | Predicted | Predicted |
| Rat | Predicted | Predicted |
| Cow | Predicted | Predicted |
| Xenopus laevis | Predicted | Predicted |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/50.00000 - 1/200.00000 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Cow, Xenopus laevis | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 0.25000-2.00000 µg/mL | Notes Fixation/Permeabilization: PFA/Triton X-100. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Cow, Xenopus laevis | Dilution info - | Notes - |
Target data
Function
Mitochondrial intermembrane chaperone that participates in the import and insertion of multi-pass transmembrane proteins into the mitochondrial inner membrane. May also be required for the transfer of beta-barrel precursors from the TOM complex to the sorting and assembly machinery (SAM complex) of the outer membrane. Acts as a chaperone-like protein that protects the hydrophobic precursors from aggregation and guide them through the mitochondrial intermembrane space.
Alternative names
Mitochondrial import inner membrane translocase subunit Tim9, TIM9A, TIMM9, TIM9, TIMM9A
Recommended products
Rabbit Polyclonal TIMM9 antibody. Suitable for IHC-P, ICC/IF and reacts with Human samples. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human TIMM9.
Alternative names
Mitochondrial import inner membrane translocase subunit Tim9, TIM9A, TIMM9, TIM9, TIMM9A
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine)- Form
Liquid
- Clonality
Polyclonal
- Immunogen
- Recombinant Full Length Protein corresponding to Human TIMM9. Database link Q9Y5J7
- Purification technique
Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage duration
1-2 weeks
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
TIMM9 also known as TINY TIM9 or TIM9 is a part of the TIMM complex that plays an essential role in mitochondrial protein import. It is a small protein with a molecular mass of approximately 10 kDa. TIMM9 is expressed mainly in mitochondria and is involved in the important process of transferring precursor proteins from the TOM complex to the TIM22 translocase within the inner mitochondrial membrane. This transfer ensures that proteins are correctly integrated and functional within the mitochondrial inner membrane.
- Biological function summary
TIMM9 operates as part of the TIMM9-TIMM10 complex which is integral for the import and assembly of mitochondrial carrier proteins. This assembly occurs within the mitochondrial intermembrane space aiding in the continuation of oxidative phosphorylation and energy production by maintaining the mitochondrial carrier proteins’ activity. The TIMM9-TIMM10 complex therefore facilitates the stability and functionality of mitochondrial membranes important for cellular metabolism and energy homeostasis.
- Pathways
TIMM9 is significantly involved in the protein translocation pathway specific to mitochondria. It functions closely with the TIM22 translocase serving as an escort for protein precursors. In this pathway TIMM9 collaborates alongside TIMM10 forming an essential step in the translocation of hydrophobic precursor proteins important for mitochondrial activity. This collaboration ensures the integrity of importing key proteins that drive processes like ATP production and metabolic regulation within mitochondria.
- Associated diseases and disorders
Mutations or dysfunctions in TIMM9 are linked to mitochondrial disorders that affect energy metabolism. Disorders such as Mitochondrial Encephalomyopathy can arise from a deficiency in the TIMM9-TIMM10 functional complex causing impaired protein import and oxidative phosphorylation. TIMM9 is also interconnected with disorders involving TIMM10 since they jointly facilitate mitochondrial function indicating that disruptions in either protein can lead to related pathophysiological conditions where energy metabolizing tissues like those in the brain and muscles are most affected.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
2 product images
Immunocytochemistry/ Immunofluorescence - Anti-TIMM9 antibody (ab224320)
PFA-fixed/Triton X-100 permeabilized U-251 MG (human brain glioma cell line) cells stained for TIMM9 (green) using ab224320 at 4 μg/ml in ICC/IF.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TIMM9 antibody (ab224320)
Paraffin-embedded human stomach tissue stained for TIMM9 using ab224320 at 1/50 dilution in immunohistochemical analysis.
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com