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AB249690

Anti-TIMMDC1/C3orf1 antibody [EPR11807] - BSA and Azide free

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Rabbit Recombinant Monoclonal TIMMDC1/C3orf1 antibody. Carrier free. Suitable for ICC/IF, IP, WB and reacts with Human samples.

View Alternative Names

C3orf1, UNQ247/PRO284, TIMMDC1, Protein M5-14, Translocase of inner mitochondrial membrane domain-containing protein 1, TIMM domain containing-protein 1

3 Images
Immunocytochemistry/ Immunofluorescence - Anti-TIMMDC1/C3orf1 antibody [EPR11807] - BSA and Azide free (AB249690)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-TIMMDC1/C3orf1 antibody [EPR11807] - BSA and Azide free (AB249690)

This data was developed using ab171978, the same antibody clone in a different buffer formulation.

Immunofluorescent analysis of HepG2 cells labeling TIMMDC1/C3orf1 with ab171978 at 1/100 dilution (red). DAPI nuclear staining (blue).

Immunoprecipitation - Anti-TIMMDC1/C3orf1 antibody [EPR11807] - BSA and Azide free (AB249690)
  • IP

Supplier Data

Immunoprecipitation - Anti-TIMMDC1/C3orf1 antibody [EPR11807] - BSA and Azide free (AB249690)

This data was developed using ab171978, the same antibody clone in a different buffer formulation.

Western blot analysis on immunoprecipitation pellet from A375 cell lysate labeling TIMMDC1/C3orf1 with ab171978 at 1/10 dilution.

All lanes:

Immunoprecipitation - Anti-TIMMDC1/C3orf1 antibody [EPR11807] (<a href='/en-us/products/primary-antibodies/timmdc1-c3orf1-antibody-epr11807-ab171978'>ab171978</a>)

Predicted band size: 32 kDa

false

Western blot - Anti-TIMMDC1/C3orf1 antibody [EPR11807] - BSA and Azide free (AB249690)
  • WB

Supplier Data

Western blot - Anti-TIMMDC1/C3orf1 antibody [EPR11807] - BSA and Azide free (AB249690)

This data was developed using ab171978, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-TIMMDC1/C3orf1 antibody [EPR11807] (<a href='/en-us/products/primary-antibodies/timmdc1-c3orf1-antibody-epr11807-ab171978'>ab171978</a>) at 1/10000 dilution

Lane 1:

THP1 cell lysate at 10 µg

Lane 2:

U937 (Human histiocytic lymphoma cell line) cell lysate at 10 µg

Lane 3:

A375 cell lysate at 10 µg

Lane 4:

HepG2 cell lysate at 10 µg

Lane 5:

Fetal heart tissue lysate at 10 µg

Predicted band size: 32 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR11807

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

IP, WB, ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Mouse": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Rat": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" } } }
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Product details

ab249690 is the carrier-free version of ab171978.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

TIMMDC1 also known as C3orf1 is a mitochondrial protein with a mass of approximately 34 kDa. It plays a mechanical role in the assembly of mitochondrial respiratory chain complex I. It becomes integrated into the inner mitochondrial membrane where it acts as an important assembly factor. TIMMDC1 is expressed ubiquitously across various human tissues with relatively higher levels in tissues with high energy demands like the heart and skeletal muscle.
Biological function summary

TIMMDC1 is essential in maintaining cellular energy metabolism. It is a component of the mitochondrial complex I assembly pathway critical for the formation and stabilization of the entire complex I. Complex I is responsible for the initial steps of the electron transport chain important for ATP production via oxidative phosphorylation. TIMMDC1 interacts with other complex I assembly factors contributing to the efficient generation of ATP within mitochondria.

Pathways

TIMMDC1 has a significant impact on the oxidative phosphorylation pathway and is a pivotal part of the mitochondrial respiratory chain. Its activity influences cellular respiration by assisting the functional integrity of complex I which interacts closely with other oxidative phosphorylation participants such as NADH dehydrogenase (ubiquinone) and cytochrome c oxidase. TIMMDC1 plays its role alongside these proteins facilitating the production of energy that cells require for various metabolic processes.

Defects in TIMMDC1 have been linked to mitochondrial complex I deficiency a condition that can result in a spectrum of disorders such as Leigh syndrome and other encephalomyopathies. This protein's malfunction can disrupt normal energy production leading to energy deficits in cells particularly in the nervous system and muscles. Additionally disruptions in the TIMMDC1 protein interaction with other mitochondrial proteins can exacerbate disease phenotypes such as with NDUFAF3 another mitochondrial assembly factor associated with complex I deficiency.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Chaperone protein involved in the assembly of the mitochondrial NADH : ubiquinone oxidoreductase complex (complex I). Participates in constructing the membrane arm of complex I.
See full target information TIMMDC1
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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