AB38978

Anti-TIMP1 antibody - Carboxyterminal end

Name: Anti-TIMP1 antibody - Carboxyterminal end (ab38978) | Abcam
Brand: Abcam Limited
SKU: AB38978
Price: 585 USD
Availability: InStock
Rating: 4 (4 reviews)

(4 Reviews)

(39 Publications)

Rabbit Polyclonal TIMP1 antibody. Suitable for WB, sELISA and reacts with Human, Recombinant full length protein - Human samples. Cited in 39 publications. Immunogen corresponding to Synthetic Peptide within Human TIMP1.

View Alternative Names

CLGI, TIMP, TIMP1, Metalloproteinase inhibitor 1, Erythroid-potentiating activity, Fibroblast collagenase inhibitor, Tissue inhibitor of metalloproteinases 1, EPA, Collagenase inhibitor, TIMP-1

3 Images

AbReview18962****

Western blot - Anti-TIMP1 antibody - Carboxyterminal end (AB38978)

Each lane corresponds to a distinct lung adenocarcinoma patient sample. The primary antibody was used at 1/3000 dilution. 10μg/ml lysate has been added per lane. A sheep anti-rabbit monoclonal conjugated to HRP was used as the secondary antibody at 1/10,000 dilution.

Detection method used was Western Lightning. 3 minutes. Performed under reducing conditions.

Samples blocked using 5% BSA for 1 hour at 25°C.

Non-specific band at 27kDa is thought to be Ig light chain.

All lanes:

Western blot - Anti-TIMP1 antibody - Carboxyterminal end (ab38978)

Predicted band size: 23 kDa

Observed band size: 27 kDa,30-35 kDa

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Image courtesy of Koji Ueda by Abreview

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Western blot - Anti-TIMP1 antibody - Carboxyterminal end (AB38978)

All lanes:

Western blot - Anti-TIMP1 antibody - Carboxyterminal end (ab38978) at 1/1000 dilution

Lane 1:

Human TIMP1

Lane 2:

Cell media from human chondrosarcoma (no treatment)

Lane 3:

Cell media from human chondrosarcoma (treated with TPA)

Predicted band size: 23 kDa

Observed band size: 29 kDa

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Sandwich ELISA - Anti-TIMP1 antibody - Carboxyterminal end (AB38978)

sELISA

Unknown

Sandwich ELISA - Anti-TIMP1 antibody - Carboxyterminal end (AB38978)

Standard curve for TIMP1 (Analyte : ab82104); dilution range 1pg/ml to 1μg/ml using Capture Antibody Mouse monoclonal [2E7.1] to TIMP1 (ab28261) at 1μg/ml and Detector Antibody Rabbit polyclonal to TIMP1 - Carboxyterminal end (ab38978) at 0.5μg/ml.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

sELISA, WB

applications

Immunogen

Synthetic Peptide within Human TIMP1. The exact immunogen used to generate this antibody is proprietary information.

P01033

Specificity

This antibody recognizes both reduced and non reduced TIMP1 protein, but does not cross react with other TIMP family members (TIMP2, TIMP3, TIMP4).

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "sELISA" : {"fullname" : "Sandwich ELISA", "shortname":"sELISA"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "<p>Recommended starting dilution of 1/1000 when using colorimetric substrates such as BCIP/NBT and 1/5000 for chemiluminescent substrates. Detects a band of approximately 29 kDa when used against the reduced protein. Higher concentration of antibody may be needed for non human samples. Dilution optimised using Chromogenic detection.</p>", "sELISA-species-checked": "guaranteed", "sELISA-species-dilution-info": "", "sELISA-species-notes": "" }, "Recombinant full length protein - Human": { "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "sELISA-species-checked": "testedAndGuaranteed", "sELISA-species-dilution-info": "", "sELISA-species-notes": "<p>For sandwich ELISA, use this antibody as Detection at 0.5μg/ml with <a href='/en-us/products/unavailable/timp1-antibody-2e71-ab28261'>ab28261</a> as Capture.</p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

Preservative: 0.05% Sodium azide Constituents: 50% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Tissue Inhibitor of Metalloproteinase 1 (TIMP1) is a protein with a molecular mass of approximately 28 kDa. The protein inhibits metalloproteinases by binding to them preventing the breakdown of the extracellular matrix. This action regulates extracellular environment and tissue remodeling. TIMP1 is also known by the name Erythroid-Potentiating Activity (EPA). It is expressed in various tissues including the liver and the brain and can be found in high levels in the blood serum.

Biological function summary

TIMP1 plays several essential roles beyond inhibiting metalloproteinases. It supports cell proliferation and influences apoptosis. It is part of a larger TIMP family and does not function as part of a complex per se but interacts closely with matrix metalloproteinases (MMPs). By regulating MMP activity TIMP1 balances processes like tissue growth and inflammatory responses which are important for normal development and repair.

Pathways

TIMP1 participates in the regulation of the matrix metalloproteinase pathway impacting tissue homeostasis and repair. It closely associates with proteins such as MMP-9 and MMP-2 within this pathway. TIMP1 also plays a role in cytokine signaling pathways which influence immune responses by interacting with other signaling molecules and receptors that govern these pathways.

TIMP1's regulation of MMPs links it to cancer and cardiovascular disease progression. Overexpression of TIMP1 associates with tumor growth and metastasis where it can modulate interactions with other proteins like MMP-9 leading to altered tissue invasion and angiogenesis. Additionally TIMP1 imbalance connects to tissue fibrosis in cardiovascular disorders working in tandem with proteins such as MMP-2 which affects the structural remodeling and function of tissues within the cardiovascular system.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Metalloproteinase inhibitor that functions by forming one to one complexes with target metalloproteinases, such as collagenases, and irreversibly inactivates them by binding to their catalytic zinc cofactor. Acts on MMP1, MMP2, MMP3, MMP7, MMP8, MMP9, MMP10, MMP11, MMP12, MMP13 and MMP16. Does not act on MMP14. Also functions as a growth factor that regulates cell differentiation, migration and cell death and activates cellular signaling cascades via CD63 and ITGB1. Plays a role in integrin signaling. Mediates erythropoiesis in vitro; but, unlike IL3, it is species-specific, stimulating the growth and differentiation of only human and murine erythroid progenitors.

See full target information TIMP1

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Publications (39)

Recent publications for all applications. Explore the full list and refine your search

Diabetes & metabolism journal 49:194-209 PubMed39721589

2024

Kidney Gastrin/CCKBR Attenuates Type 2 Diabetes Mellitus by Inhibiting SGLT2-Mediated Glucose Reabsorption through Erk/NF-κB Signaling Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Xue Zhang,Yuhan Zhang,Yang Shi,Dou Shi,Min Niu,Xue Liu,Xing Liu,Zhiwei Yang,Xianxian Wu

Cancer discovery 15:179-201 PubMed39354883

2024

TIMP1 Mediates Astrocyte-Dependent Local Immunosuppression in Brain Metastasis Acting on Infiltrating CD8+ T Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Neibla Priego,Ana de Pablos-Aragoneses,María Perea-García,Valentina Pieri,Carolina Hernández-Oliver,Laura Álvaro-Espinosa,Andrea Rojas,Oliva Sánchez,Ariane Steindl,Eduardo Caleiras,Fernando García,Santiago García-Martín,Osvaldo Graña-Castro,Sandra García-Mulero,Diego Serrano,Paloma Velasco-Beltrán,Borja Jiménez-Lasheras,Leire Egia-Mendikute,Luise Rupp,Antonia Stammberger,Matthias Meinhardt,Anas Chaachou-Charradi,Elena Martínez-Saez,Luca Bertero,Paola Cassoni,Luca Mangherini,Alessia Pellerino,Roberta Rudà,Riccardo Soffietti,Fatima Al-Shahrour,Paul Saftig,Rebeca Sanz-Pamplona,Marc Schmitz,Stephen J Crocker,Alfonso Calvo,Asís Palazón,Manuel Valiente

Journal of cardiovascular translational research 16:3-16 PubMed36197585

2022

Harnessing the Plasma Proteome to Mirror Current and Predict Future Cardiac Remodeling After Myocardial Infarction.

Applications

Unspecified application

Species

Unspecified reactive species

Upendra Chalise,Mediha Becirovic-Agic,Jocelyn R Rodriguez-Paar,Shelby R Konfrst,Sharon D B de Morais,Catherine S Johnson,Elizabeth R Flynn,Michael E Hall,Daniel R Anderson,Leah M Cook,Kristine Y DeLeon-Pennell,Merry L Lindsey

Frontiers in pharmacology 13:934136 PubMed36059948

2022

Dapagliflozin protects against nonalcoholic steatohepatitis in db/db mice.

Applications

Unspecified application

Species

Unspecified reactive species

Panshuang Qiao,Yingli Jia,Ang Ma,Jinzhao He,Chen Shao,Xiaowei Li,Shuyuan Wang,Baoxue Yang,Hong Zhou

American journal of translational research 13:11329-11340 PubMed34786061

2021

Oncostatin M promotes infarct repair and improves cardiac function after myocardial infarction.

Applications

Unspecified application

Species

Unspecified reactive species

Hui Han,Daopeng Dai,Run Du,Jinquan Hu,Zhengbin Zhu,Lin Lu,Jinzhou Zhu,Ruiyan Zhang

British journal of pharmacology 178:2424-2442 PubMed33660265

2021

Simultaneous targeting of oxidative stress and fibrosis reverses cardiomyopathy-induced ventricular remodelling and dysfunction.

Applications

Unspecified application

Species

Unspecified reactive species

Chao Wang,Tracey A Gaspari,Dorota Ferens,Iresha Spizzo,Barbara K Kemp-Harper,Chrishan S Samuel

International journal of molecular medicine 46:2035-2046 PubMed33125088

2020

Resveratrol contributes to the inhibition of liver fibrosis by inducing autophagy via the microRNA‑20a‑mediated activation of the PTEN/PI3K/AKT signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Lili Zhu,Qiuju Mou,Yinghui Wang,Zixin Zhu,Mingliang Cheng

International journal of molecular medicine 46:1074-1084 PubMed32705163

2020

Activation of SIRT1 promotes cartilage differentiation and reduces apoptosis of nucleus pulposus mesenchymal stem cells via the MCP1/CCR2 axis in subjects with intervertebral disc degeneration.

Applications

Unspecified application

Species

Unspecified reactive species

Xuancheng Ou,Jinwei Ying,Xuedong Bai,Chaofeng Wang,Dike Ruan

General physiology and biophysics 39:229-237 PubMed32525816

2020

The circRNA-MYLK plays oncogenic roles in the Hep-2 cell line by sponging microRNA-145-5p.

Applications

Unspecified application

Species

Unspecified reactive species

Yao Chen,Yanmei Wang,Yanmei Li,Xuechan Li,Tiejun Yuan,Shuqin Yang,Xiaoyan Sun

Neurourology and urodynamics 39:1292-1303 PubMed32330364

2020

β-Adrenoceptors regulate matrix metalloproteinase expression in human urothelial cells under hydrostatic pressure.

Applications

Unspecified application

Species

Unspecified reactive species

Jianhua Lan,Tao Jin,Jianzhong Ai,Xin Wei,Zhihui Huang,Hua Chen,Xi Jin,Zhumei Luo,Kunjie Wang

View all publications

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