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AB179580

Anti-TIMP1 antibody [EPR16616]

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(14 Publications)

Rabbit Recombinant Monoclonal TIMP1 antibody. Suitable for WB and reacts with Mouse samples. Cited in 14 publications.

View Alternative Names

Timp, Timp-1, Timp1, Metalloproteinase inhibitor 1, Collagenase inhibitor 16C8 fibroblast, Erythroid-potentiating activity, TPA-S1, TPA-induced protein, Tissue inhibitor of metalloproteinases 1, EPA, TIMP-1

1 Images
Western blot - Anti-TIMP1 antibody [EPR16616] (AB179580)
  • WB

Supplier Data

Western blot - Anti-TIMP1 antibody [EPR16616] (AB179580)

Blocking / Dilution : 5% NFDM/TBST

Exposure time : Lane 1 : 32 seconds; Lane 2 : 3 minutes

The molecular mass observed is consistent with the literature (PMID : 17202148).

All lanes:

Western blot - Anti-TIMP1 antibody [EPR16616] (ab179580) at 1/1000 dilution

Lane 1:

Mouse lung culture supernatant (7 days) at 20 µL

Lane 2:

Mouse leg bone lysate at 10 µg

Secondary

Lane 1:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Lane 2:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 23 kDa

Observed band size: 28 kDa

true

  • Carrier free

    Anti-TIMP1 antibody [EPR16616] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR16616

Isotype

IgG

Carrier free

No

Reacts with

Mouse

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Tissue Inhibitor of Metalloproteinase 1 (TIMP1) is a protein with a molecular mass of approximately 28 kDa. The protein inhibits metalloproteinases by binding to them preventing the breakdown of the extracellular matrix. This action regulates extracellular environment and tissue remodeling. TIMP1 is also known by the name Erythroid-Potentiating Activity (EPA). It is expressed in various tissues including the liver and the brain and can be found in high levels in the blood serum.
Biological function summary

TIMP1 plays several essential roles beyond inhibiting metalloproteinases. It supports cell proliferation and influences apoptosis. It is part of a larger TIMP family and does not function as part of a complex per se but interacts closely with matrix metalloproteinases (MMPs). By regulating MMP activity TIMP1 balances processes like tissue growth and inflammatory responses which are important for normal development and repair.

Pathways

TIMP1 participates in the regulation of the matrix metalloproteinase pathway impacting tissue homeostasis and repair. It closely associates with proteins such as MMP-9 and MMP-2 within this pathway. TIMP1 also plays a role in cytokine signaling pathways which influence immune responses by interacting with other signaling molecules and receptors that govern these pathways.

TIMP1's regulation of MMPs links it to cancer and cardiovascular disease progression. Overexpression of TIMP1 associates with tumor growth and metastasis where it can modulate interactions with other proteins like MMP-9 leading to altered tissue invasion and angiogenesis. Additionally TIMP1 imbalance connects to tissue fibrosis in cardiovascular disorders working in tandem with proteins such as MMP-2 which affects the structural remodeling and function of tissues within the cardiovascular system.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Metalloproteinase inhibitor that functions by forming one to one complexes with target metalloproteinases, such as collagenases, and irreversibly inactivates them by binding to their catalytic zinc cofactor. Acts on MMP1, MMP2, MMP3, MMP7, MMP8, MMP9, MMP10, MMP11, MMP12, MMP13 and MMP16. Does not act on MMP14 (By similarity). Also functions as a growth factor that regulates cell differentiation, migration and cell death and activates cellular signaling cascades via CD63 and ITGB1. Plays a role in integrin signaling.
See full target information Timp1
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Publications (14)

Recent publications for all applications. Explore the full list and refine your search

Scientific reports 15:28482 PubMed40759713

2025

The inhibitory effect of M2 macrophage-derived exosomes on gefitinib resistant lung adenocarcinoma cells through the MIF/TIMP1/CD74 axis.

Applications

Unspecified application

Species

Unspecified reactive species

Jingcui Peng,Yan Zhang,Bin Li,Xin He,Cuimin Ding,Wenxia Hu

Hereditas 162:119 PubMed40604895

2025

TIMP1 promotes microglia M2 polarization through MAPK pathway to ameliorate early brain injury after ischemia.

Applications

Unspecified application

Species

Unspecified reactive species

Kangkang Zhao,Zizhao Huang

Journal of cellular and molecular medicine 29:e70579 PubMed40387631

2025

Effects of Different Doses of Glucosamine Hydrochloride on Cartilage Tissue and Levels of Joint Injury Markers in Knee Osteoarthritis.

Applications

Unspecified application

Species

Unspecified reactive species

Xichun Wang,Bin Hu,Yi Cheng,Wenjie Chen,Muzi Liu

Heliyon 10:e30240 PubMed38726105

2024

Calycosin prevents NLRP3-induced gut fibrosis by regulating IL-33/ST2 axis.

Applications

Unspecified application

Species

Unspecified reactive species

Xiujun Liao,Haiting Xie,Saojun Yu

Respiratory research 25:51 PubMed38254111

2024

Tobacco-derived and tobacco-free nicotine cause differential inflammatory cell influx and MMP-9 in mouse lung.

Applications

Unspecified application

Species

Unspecified reactive species

Thomas Lamb,Gagandeep Kaur,Irfan Rahman

Sleep & breathing = Schlaf & Atmung 28:697-705 PubMed37923872

2023

Relationship between circadian rhythm-related genes and extracellular matrix: implications for sleep deprivation.

Applications

Unspecified application

Species

Unspecified reactive species

Xuejuan Liu,Jia Sun,Zexia Ling,Tong Dong

Nature communications 14:1829 PubMed37005411

2023

Parabacteroides distasonis ameliorates hepatic fibrosis potentially via modulating intestinal bile acid metabolism and hepatocyte pyroptosis in male mice.

Applications

Unspecified application

Species

Unspecified reactive species

Qi Zhao,Man-Yun Dai,Ruo-Yue Huang,Jing-Yi Duan,Ting Zhang,Wei-Min Bao,Jing-Yi Zhang,Shao-Qiang Gui,Shu-Min Xia,Cong-Ting Dai,Ying-Mei Tang,Frank J Gonzalez,Fei Li

Journal of cardiovascular translational research 16:3-16 PubMed36197585

2022

Harnessing the Plasma Proteome to Mirror Current and Predict Future Cardiac Remodeling After Myocardial Infarction.

Applications

Unspecified application

Species

Unspecified reactive species

Upendra Chalise,Mediha Becirovic-Agic,Jocelyn R Rodriguez-Paar,Shelby R Konfrst,Sharon D B de Morais,Catherine S Johnson,Elizabeth R Flynn,Michael E Hall,Daniel R Anderson,Leah M Cook,Kristine Y DeLeon-Pennell,Merry L Lindsey

Pathology international 72:506-518 PubMed36066006

2022

Histological characteristics of matrix metalloproteinase-9 and tissue inhibitor of metalloproteinases-1 in asthmatic murine model during A(H1N1)pdm09 infection.

Applications

Unspecified application

Species

Unspecified reactive species

Sasagu Kimura,Hiroki Yasudo,Atsunori Oga,Reiji Fukano,Takeshi Matsushige,Hiroki Hamano,Hideki Hasegawa,Noriko Nakajima,Akira Ainai,Hiroshi Itoh,Komei Shirabe,Shoichi Toda,Ryo Atsuta,Shunji Hasegawa

Journal of inflammation research 15:4693-4708 PubMed35996686

2022

Ezh2 Inhibits Replicative Senescence of Atrial Fibroblasts Through Promotion of H3K27me3 in the Promoter Regions of CDKN2a and Timp4 Genes.

Applications

Unspecified application

Species

Unspecified reactive species

Yingze Li,Guojian Fang,Wei Cao,Jiali Yuan,Shuai Song,Hong Peng,Yuepeng Wang,Qunshan Wang
View all publications
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