AB86482

Anti-TIMP1 antibody [RM0136-6A34]

Name: Anti-TIMP1 antibody [RM0136-6A34] (ab86482) | Abcam
Brand: Abcam Limited
SKU: AB86482

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(13 Publications)

Rat Monoclonal TIMP1 antibody. Suitable for WB, IHC-Fr and reacts with Mouse samples. Cited in 13 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Mouse Timp1.

View Alternative Names

Timp, Timp-1, Timp1, Metalloproteinase inhibitor 1, Collagenase inhibitor 16C8 fibroblast, Erythroid-potentiating activity, TPA-S1, TPA-induced protein, Tissue inhibitor of metalloproteinases 1, EPA, TIMP-1

1 Images

Supplier Data

Western blot - Anti-TIMP1 antibody [RM0136-6A34] (AB86482)

All lanes:

Western blot - Anti-TIMP1 antibody [RM0136-6A34] (ab86482) at 1/500 dilution

All lanes:

Mouse embryonic tissue

Predicted band size: 23 kDa

false

Key facts

Host species

Rat

Clonality

Monoclonal

Clone number

RM0136-6A34

Isotype

IgG2

Carrier free

Reacts with

Mouse

Applications

IHC-Fr, WB

applications

Immunogen

Recombinant Full Length Protein corresponding to Mouse Timp1.

P12032

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "<p></p>", "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "<p></p>" } } }

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Properties and storage information

Form

Liquid

Purification notes

The IgG fraction of culture supernatant was purified by protein A/G affinity chromatography and 0.2µm filtered.

Storage buffer

Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Tissue Inhibitor of Metalloproteinase 1 (TIMP1) is a protein with a molecular mass of approximately 28 kDa. The protein inhibits metalloproteinases by binding to them preventing the breakdown of the extracellular matrix. This action regulates extracellular environment and tissue remodeling. TIMP1 is also known by the name Erythroid-Potentiating Activity (EPA). It is expressed in various tissues including the liver and the brain and can be found in high levels in the blood serum.

Biological function summary

TIMP1 plays several essential roles beyond inhibiting metalloproteinases. It supports cell proliferation and influences apoptosis. It is part of a larger TIMP family and does not function as part of a complex per se but interacts closely with matrix metalloproteinases (MMPs). By regulating MMP activity TIMP1 balances processes like tissue growth and inflammatory responses which are important for normal development and repair.

Pathways

TIMP1 participates in the regulation of the matrix metalloproteinase pathway impacting tissue homeostasis and repair. It closely associates with proteins such as MMP-9 and MMP-2 within this pathway. TIMP1 also plays a role in cytokine signaling pathways which influence immune responses by interacting with other signaling molecules and receptors that govern these pathways.

TIMP1's regulation of MMPs links it to cancer and cardiovascular disease progression. Overexpression of TIMP1 associates with tumor growth and metastasis where it can modulate interactions with other proteins like MMP-9 leading to altered tissue invasion and angiogenesis. Additionally TIMP1 imbalance connects to tissue fibrosis in cardiovascular disorders working in tandem with proteins such as MMP-2 which affects the structural remodeling and function of tissues within the cardiovascular system.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Metalloproteinase inhibitor that functions by forming one to one complexes with target metalloproteinases, such as collagenases, and irreversibly inactivates them by binding to their catalytic zinc cofactor. Acts on MMP1, MMP2, MMP3, MMP7, MMP8, MMP9, MMP10, MMP11, MMP12, MMP13 and MMP16. Does not act on MMP14 (By similarity). Also functions as a growth factor that regulates cell differentiation, migration and cell death and activates cellular signaling cascades via CD63 and ITGB1. Plays a role in integrin signaling.

See full target information Timp1

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Publications (13)

Recent publications for all applications. Explore the full list and refine your search

International journal of molecular sciences 23: PubMed36498987

2022

Immunoproteasome Inhibition Ameliorates Aged Dystrophic Mouse Muscle Environment.

Applications

Unspecified application

Species

Unspecified reactive species

Luana Tripodi,Davide Molinaro,Francesco Fortunato,Carolina Mella,Barbara Cassani,Yvan Torrente,Andrea Farini

Journal of physiology and pharmacology : an offici 71: PubMed32776909

2020

Effect of natalizumab treatment on metalloproteinases and their inhibitors in a mouse model of multiple sclerosis.

Applications

Unspecified application

Species

Unspecified reactive species

G Pyka-Fosciak,G J Lis,J A Litwin

Frontiers in immunology 11:480 PubMed32296422

2020

LOXL2 Inhibition Paves the Way for Macrophage-Mediated Collagen Degradation in Liver Fibrosis.

Applications

Unspecified application

Species

Unspecified reactive species

Mordehay Klepfish,Tamar Gross,Milena Vugman,Nikolaos A Afratis,Sapir Havusha-Laufer,Eli Brazowski,Inna Solomonov,Chen Varol,Irit Sagi

Cancer cell international 20:49 PubMed32082079

2020

Hsa_circ_0011385 accelerates the progression of thyroid cancer by targeting miR-361-3p.

Applications

Unspecified application

Species

Unspecified reactive species

Fada Xia,Yong Chen,Bo Jiang,Ning Bai,Xinying Li

International journal of biological sciences 16:194-203 PubMed31929748

2020

Ruxolitinib Alleviates Renal Interstitial Fibrosis in UUO Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Yu Bai,Wei Wang,Ping Yin,Jian Gao,Lei Na,Yu Sun,Zhuo Wang,Zhongbo Zhang,Chenghai Zhao

Frontiers in pharmacology 10:1486 PubMed31920668

2019

Thalidomide Prevented and Ameliorated Pathogenesis of Crohn's Disease in Mice Regulation of Inflammatory Response and Fibrosis.

Applications

Unspecified application

Species

Unspecified reactive species

Hongjin Chen,Haixia Xu,Lijiao Luo,Lichao Qiao,Yaohui Wang,Minmin Xu,Youran Li,Ping Zhu,Bolin Yang

The Journal of neuroscience : the official journal 39:4387-4402 PubMed30926752

2019

CD73 Promotes Glioblastoma Pathogenesis and Enhances Its Chemoresistance via A Adenosine Receptor Signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Angela Yan,Michelle L Joachims,Linda F Thompson,Andrew D Miller,Peter D Canoll,Margaret S Bynoe

Oncology reports 40:2287-2297 PubMed30106431

2018

α7‑nAchR agonist GTS‑21 reduces radiation‑induced lung injury.

Applications

Unspecified application

Species

Unspecified reactive species

Zijie Mei,Xiaoli Tian,Jing Chen,Yacheng Wang,Ye Yao,Xin Li,Chunxu Yang,Shimin Zhang,Conghua Xie

Journal of the American Heart Association 5: PubMed27993833

2016

Nkx2-5 Is Expressed in Atherosclerotic Plaques and Attenuates Development of Atherosclerosis in Apolipoprotein E-Deficient Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Meng Du,Xiaojing Wang,Xin Tan,Xiangrao Li,Dandan Huang,Kun Huang,Liu Yang,Fengxiao Zhang,Yan Wang,Dan Huang,Kai Huang

Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology 39:1521-36 PubMed27614871

2016

Activation of Cannabinoid Receptor Type II by AM1241 Ameliorates Myocardial Fibrosis via Nrf2-Mediated Inhibition of TGF-β1/Smad3 Pathway in Myocardial Infarction Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Xiang Li,Dong Han,Zuhong Tian,Beilei Gao,Miaomiao Fan,Congye Li,Xiujuan Li,Yabin Wang,Sai Ma,Feng Cao

View all publications

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