Anti-TIMP2 antibody (ab180630) is a rabbit polyclonal antibody that is used to detect TIMP2 in Western Blot, IHC-P, ICC/IF. Suitable for Human, Mouse, Rat samples.
- Over 50 publications
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- Molecular medicine (Cambridge, Mass.) 29:1272023Quercetin inhibits the metabolism of arachidonic acid by inhibiting the activity of CYP3A4, thereby inhibiting the progression of breast cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHuaming Tang,Yuanli Kuang,Wan Wu,Bing Peng,Qianmei FuPubMed 37710176
- Cardiorenal medicine 13:221-2312023Serum Insulin-Like Growth Factor-Binding Protein 7 Deriving from Spleen and Lung Could Be Used for Early Recognition of Cardiac Surgery-Associated Acute Kidney Injury.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYimei Wang,Bo Shen,Xuesen Cao,Zhihui Lu,Yang Zhang,Bowen Zhu,Weidong Zhang,Yiqin Shi,Jialin Wang,Yi Fang,Nana Song,Yang Li,Xialian Xu,Ping Jia,Xiaoqiang Ding,Shuan ZhaoPubMed 37311433
- PloS one 18:e02848752023A standardized method for plasma extracellular vesicle isolation and size distribution analysis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJ Nathaniel Diehl,Amelia Ray,Lauren B Collins,Andrew Peterson,Kyle C Alexander,Jacob G Boutros,John S Ikonomidis,Adam W AkermanPubMed 37115777
- Cell death & disease 13:10322022Cancer-derived exosomal miR-197-3p confers angiogenesis via targeting TIMP2/3 in lung adenocarcinoma metastasis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRui-Min Chang,Yao Fu,Jun Zeng,Xiao-Yan Zhu,Yang GaoPubMed 36494333
- Oncology reports 48:2022OTX1 promotes tumorigenesis and progression of cervical cancer by regulating the Wnt signaling pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLimin Zhou,Hongying Li,Dunlan Zhang,Lu Chen,Hong Dong,Yuqin Yuan,Tinghui WangPubMed 36177903
- Regenerative biomaterials 9:rbac0742022Tannic acid-loaded hydrogel coating endues polypropylene mesh with hemostatic and anti-inflammatory capacity for facilitating pelvic floor repair.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChenghao Wu,Zixuan Zhou,Xi You,Yi Guo,Ping Chen,Huaifang Li,Xiaowen TongPubMed 36267152
- Frontiers in pharmacology 13:9224772022Inhibitory effects of total triterpenoids isolated from the willd on H1975 cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKenan Wu,Xue Wu,Yanni Liang,Ting Wang,Dongzhi Wu,Luhan Li,Zheng WangPubMed 36188592
- Molecules (Basel, Switzerland) 27:2022Improved Wound Healing by Naringin Associated with MMP and the VEGF Pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJia-Hau Yen,Wan-Ting Chio,Chia-Ju Chuang,Hsin-Ling Yang,Sheng-Teng HuangPubMed 35268795
- FASEB journal : official publication of the Federation of American Societies for Experimental Biology 36:e222282022TIMP2 mediates endoplasmic reticulum stress contributing to sepsis-induced acute kidney injury.Applications:Unspecified applicationReactive species:Unspecified reactive speciesNanhui Jiang,Rong Huang,Jiahao Zhang,Dongxue Xu,Tianlong Li,Zhongyi Sun,Lianjiu Su,Zhiyong PengPubMed 35218571
- Translational vision science & technology 11:302022HL3501, a Novel Selective A3 Adenosine Receptor Antagonist, Lowers Intraocular Pressure (IOP) in Animal Glaucoma Models.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYunhee Kim,Jaewook Yang,Jee Young Kim,Jang Mi Lee,Woo Chan Son,Byoung-Gon MoonPubMed 35191964
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.3
Preservative: 0.05% Proclin 300
Constituents: 50% Glycerol (glycerin, glycerine), 49.95% PBS- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Full Length Protein corresponding to Human TIMP2. Database link P16035
Reactivity data
Select an application| WB | IHC-P | ICC/IF | |
|---|---|---|---|
| Human | Tested | Expected | Expected |
| Mouse | Predicted | Expected | Expected |
| Rat | Predicted | Expected | Expected |
| Chinese hamster | Predicted | Predicted | Predicted |
| Cow | Predicted | Predicted | Predicted |
| Dog | Predicted | Predicted | Predicted |
| Guinea pig | Predicted | Predicted | Predicted |
| Rabbit | Predicted | Predicted | Predicted |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500.00000 - 1/2000.00000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rabbit, Guinea pig, Cow, Dog, Chinese hamster, Rat, Mouse | Dilution info - | Notes - |
IHC-P
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody. |
Species Rat | Dilution info - | Notes ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody. |
Species Human | Dilution info - | Notes ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rabbit, Guinea pig, Cow, Dog, Chinese hamster | Dilution info - | Notes - |
ICC/IF
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rabbit, Guinea pig, Cow, Dog, Chinese hamster | Dilution info - | Notes - |
Associated Products
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Target data
Function
Complexes with metalloproteinases (such as collagenases) and irreversibly inactivates them by binding to their catalytic zinc cofactor. Known to act on MMP-1, MMP-2, MMP-3, MMP-7, MMP-8, MMP-9, MMP-10, MMP-13, MMP-14, MMP-15, MMP-16 and MMP-19.
Alternative names
Metalloproteinase inhibitor 2, CSC-21K, Tissue inhibitor of metalloproteinases 2, TIMP-2, TIMP2
Recommended products
Anti-TIMP2 antibody (ab180630) is a rabbit polyclonal antibody that is used to detect TIMP2 in Western Blot, IHC-P, ICC/IF. Suitable for Human, Mouse, Rat samples.
- Over 50 publications
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.3
Preservative: 0.05% Proclin 300
Constituents: 50% Glycerol (glycerin, glycerine), 49.95% PBS- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Full Length Protein corresponding to Human TIMP2. Database link P16035
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Anti-TIMP2 antibody (ab180630) is a rabbit polyclonal antibody and is validated for use in Western Blot (WB), Immunohistochemistry (IHC-P), Immunocytochemistry/immunofluorescence (ICC/IF), in Human, Mouse, Rat samples.
What is the molecular weight of TIMP2?
Anti-TIMP2 (ab180630) specifically detects a band for TIMP2 (UniProt: P16035) at a molecular weight of 24kDa.
Trusted by the scientific community
Anti-TIMP2 (ab180630) was first used in a scientific publication in 2013 and has been cited over 50 times in peer-reviewed journals.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
TIMP2 also known as Tissue Inhibitor of Metalloproteinases 2 mechanistically inhibits the activity of matrix metalloproteinases (MMPs). It binds to MMPs regulating the extracellular matrix's remodeling. The protein has a molecular mass of around 21 kDa. TIMP2 is expressed in various tissues including the brain liver and kidneys where it maintains a balance between matrix degradation and production.
- Biological function summary
TIMP2 plays a significant role in tissue maintenance and repair by controlling extracellular matrix turnover. It forms complexes with MMPs such as MMP-2 inhibiting their proteolytic activities. The protein is also involved in cell proliferation and differentiation affecting processes like angiogenesis and wound healing. Its regulatory function aids in maintaining tissue homeostasis and preventing excessive matrix breakdown.
- Pathways
TIMP2 is integral to the matrix metalloproteinase pathway and the regulation of endothelial cell migration. The protein interacts closely with other MMPs influencing their activation and modulation. Through these pathways TIMP2 holds an important position in cellular migration and signaling ensuring precise matrix remodeling in response to physiological needs.
- Associated diseases and disorders
TIMP2 has significant links with cancer and fibrosis. Dysregulation of TIMP2 expression or its imbalance with MMPs can contribute to tumor progression and metastasis due to uncontrolled matrix degradation. Additionally abnormal TIMP2 levels associate with fibrotic diseases where excessive tissue deposition occurs. TIMP2's interaction with proteins like MMP-9 and MMP-2 highlights its involvement in these pathological conditions.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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In the unlikely event of one of our products not working as expected, you are covered by our product promise.
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1 product image
Western blot - Anti-TIMP2 antibody (ab180630)
All lanes: Western blot - Anti-TIMP2 antibody (ab180630) at 1/500 dilution
All lanes: SW480 cell line extract
Predicted band size: 24 kDa
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