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AB180630

Anti-TIMP2 antibody

5

(1 Review)

|

(72 Publications)

Anti-TIMP2 antibody (ab180630) is a rabbit polyclonal antibody detecting TIMP2 in Western Blot, IHC-P, ICC/IF. Suitable for Human, Mouse, Rat.

- Over 50 publications

View Alternative Names

Metalloproteinase inhibitor 2, CSC-21K, Tissue inhibitor of metalloproteinases 2, TIMP-2, TIMP2

1 Images
Western blot - Anti-TIMP2 antibody (AB180630)
  • WB

Supplier Data

Western blot - Anti-TIMP2 antibody (AB180630)

All lanes:

Western blot - Anti-TIMP2 antibody (ab180630) at 1/500 dilution

All lanes:

SW480 cell line extract

Predicted band size: 24 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human, Rat, Mouse

Applications

WB, IHC-P, ICC/IF

applications

Immunogen

Recombinant Full Length Protein corresponding to Human TIMP2.

P16035

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Alternative Products

Primary Antibodies

AB238062

Anti-TIMP2 antibody [TIMP2/2044]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TIMP2 antibody [TIMP2/2044] (AB238062)

  • 0
  • 0
Primary Antibodies

AB237913

Anti-TIMP2 antibody [TIMP2/2044] - BSA and Azide free

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TIMP2 antibody [TIMP2/2044] - BSA and Azide free (AB237913)

  • 0
  • 0
Primary Antibodies

AB53730

Anti-TIMP2 antibody

Western blot - Anti-TIMP2 antibody (AB53730)

  • 0
  • 0
Primary Antibodies

AB231176

Anti-TIMP2 antibody

Western blot - Anti-TIMP2 antibody (AB231176)

  • 0
  • 0
Primary Antibodies

AB230511

Anti-TIMP2 antibody [F27P3A4]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TIMP2 antibody [F27P3A4] (AB230511)

  • 0
  • 0
Primary Antibodies

AB1828

Anti-TIMP2 antibody [3A4]

BOND RX™ Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TIMP2 antibody [3A4] (AB1828)

  • 4
  • 7

Complementary Products

Assay Kits

AB270213

Human TIMP2 ELISA Kit

Recombinant|SimpleStep

ELISA - Human TIMP2 ELISA Kit (AB270213)

  • 5
  • 1
Primary Antibodies

AB92536

Anti-MMP2 antibody [EPR1184]

RabMAb|Recombinant

Western blot - Anti-MMP2 antibody [EPR1184] (AB92536)

  • 4
  • 6
Primary Antibodies

AB283575

Anti-MMP9 antibody [RM1020]

BOND RX™ Validated|20ul selling size|RabMAb|Recombinant|KO Validated|Trial Size

Flow Cytometry (Intracellular) - Anti-MMP9 antibody [RM1020] (AB283575)

  • 0
  • 0
Primary Antibodies

AB211926

Anti-TIMP1 antibody [EPR18352]

RabMAb|Recombinant|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TIMP1 antibody [EPR18352] (AB211926)

  • 5
  • 10
Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p><a href='/en-us/products/primary-antibodies/rabbit-igg-polyclonal-isotype-control-chip-grade-ab171870'>ab171870</a> - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.</p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p><a href='/en-us/products/primary-antibodies/rabbit-igg-polyclonal-isotype-control-chip-grade-ab171870'>ab171870</a> - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.</p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p><a href='/en-us/products/primary-antibodies/rabbit-igg-polyclonal-isotype-control-chip-grade-ab171870'>ab171870</a> - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.</p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" }, "Chinese hamster": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Dog": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Guinea pig": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rabbit": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Product details

What is this antibody validated in?
Anti-TIMP2 antibody (ab180630) is a rabbit polyclonal antibody and is validated for use in Western Blot (WB), Immunohistochemistry (IHC-P), Immunocytochemistry/immunofluorescence (ICC/IF) in Human, Mouse, Rat samples.

What is the molecular weight of TIMP2?
Anti-TIMP2 (ab180630) specifically detects a band for TIMP2 (UniProt: P16035) at a molecular weight of 24kDa.

Trusted by the scientific community
Anti-TIMP2 (ab180630) was first used in a scientific publication in 2013 and has been cited over 50 times in peer-reviewed journals.
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.3 Preservative: 0.05% Proclin 300 Constituents: PBS, 50% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

TIMP2 also known as Tissue Inhibitor of Metalloproteinases 2 mechanistically inhibits the activity of matrix metalloproteinases (MMPs). It binds to MMPs regulating the extracellular matrix's remodeling. The protein has a molecular mass of around 21 kDa. TIMP2 is expressed in various tissues including the brain liver and kidneys where it maintains a balance between matrix degradation and production.
Biological function summary

TIMP2 plays a significant role in tissue maintenance and repair by controlling extracellular matrix turnover. It forms complexes with MMPs such as MMP-2 inhibiting their proteolytic activities. The protein is also involved in cell proliferation and differentiation affecting processes like angiogenesis and wound healing. Its regulatory function aids in maintaining tissue homeostasis and preventing excessive matrix breakdown.

Pathways

TIMP2 is integral to the matrix metalloproteinase pathway and the regulation of endothelial cell migration. The protein interacts closely with other MMPs influencing their activation and modulation. Through these pathways TIMP2 holds an important position in cellular migration and signaling ensuring precise matrix remodeling in response to physiological needs.

TIMP2 has significant links with cancer and fibrosis. Dysregulation of TIMP2 expression or its imbalance with MMPs can contribute to tumor progression and metastasis due to uncontrolled matrix degradation. Additionally abnormal TIMP2 levels associate with fibrotic diseases where excessive tissue deposition occurs. TIMP2's interaction with proteins like MMP-9 and MMP-2 highlights its involvement in these pathological conditions.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Complexes with metalloproteinases (such as collagenases) and irreversibly inactivates them by binding to their catalytic zinc cofactor. Known to act on MMP-1, MMP-2, MMP-3, MMP-7, MMP-8, MMP-9, MMP-10, MMP-13, MMP-14, MMP-15, MMP-16 and MMP-19.
See full target information TIMP2
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Publications (72)

Recent publications for all applications. Explore the full list and refine your search

NPJ precision oncology 9:71 PubMed40075158

2025

Exosomes containing miR-152-3p targeting FGFR3 mediate SLC7A7-induced angiogenesis in bladder cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Chun Cao,Yu Wang,Xiaolin Deng,Xinlei Zhao,Yuwen Chen,Wanlong Tan,Fan Deng,Fei Li

Journal of cell communication and signaling 19:e12059 PubMed39975983

2025

Moscatilin alleviates oxidative stress and inflammatory response of Müller cells in diabetic retinopathy through suppressing the p38 mitogen-activated protein kinase/c-Jun N-terminal kinase and nuclear factor kappa-B signaling pathways.

Applications

Unspecified application

Species

Unspecified reactive species

Suhua Zhu,Man Zhang,Zhen Qu,Shengqiu Xu,Jie Peng,Fanjing Jiang

European journal of histochemistry : EJH 68: PubMed38934084

2024

Effects of artificial light with different spectral compositions on refractive development and matrix metalloproteinase 2 and tissue inhibitor of metalloproteinases 2 expression in the sclerae of juvenile guinea pigs.

Applications

Unspecified application

Species

Unspecified reactive species

Jianbao Yuan,Linfang Li,Yi Fan,Xinyu Xu,Xiaoqiong Huang,Jiayu Shi,Chuanwei Zhang,Lixin Shi,Yuliang Wang

Annals of translational medicine 12:43 PubMed38911554

2024

Removal of the infrapatellar fat pad and associated synovium benefits female guinea pigs in the Dunkin Hartley model of idiopathic osteoarthritis.

Applications

Unspecified application

Species

Unspecified reactive species

Maryam F Afzali,Madeline M Sykes,Lindsey H Burton,Kayley M Patton,Koryn R Lee,Cassie Seebart,Nicole Vigon,Ryan Ek,Gerardo E Narez,Angela J Marolf,Katie J Sikes,Tammy L Haut Donahue,Kelly S Santangelo

Cell reports. Medicine 5:101352 PubMed38232700

2024

IL32 downregulation lowers triglycerides and type I collagen in di-lineage human primary liver organoids.

Applications

Unspecified application

Species

Unspecified reactive species

Kavitha Sasidharan,Andrea Caddeo,Oveis Jamialahmadi,Francesca Rita Noto,Melissa Tomasi,Francesco Malvestiti,Ester Ciociola,Federica Tavaglione,Rosellina M Mancina,Alessandro Cherubini,Cristiana Bianco,Angela Mirarchi,Ville Männistö,Jussi Pihlajamäki,Vesa Kärjä,Stefania Grimaudo,Panu K Luukkonen,Sami Qadri,Hannele Yki-Järvinen,Salvatore Petta,Silvia Manfrini,Umberto Vespasiani-Gentilucci,Vincenzo Bruni,Luca Valenti,Stefano Romeo

Molecular medicine (Cambridge, Mass.) 29:127 PubMed37710176

2023

Quercetin inhibits the metabolism of arachidonic acid by inhibiting the activity of CYP3A4, thereby inhibiting the progression of breast cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Huaming Tang,Yuanli Kuang,Wan Wu,Bing Peng,Qianmei Fu

Cardiorenal medicine 13:221-231 PubMed37311433

2023

Serum Insulin-Like Growth Factor-Binding Protein 7 Deriving from Spleen and Lung Could Be Used for Early Recognition of Cardiac Surgery-Associated Acute Kidney Injury.

Applications

Unspecified application

Species

Unspecified reactive species

Yimei Wang,Bo Shen,Xuesen Cao,Zhihui Lu,Yang Zhang,Bowen Zhu,Weidong Zhang,Yiqin Shi,Jialin Wang,Yi Fang,Nana Song,Yang Li,Xialian Xu,Ping Jia,Xiaoqiang Ding,Shuan Zhao

Anais da Academia Brasileira de Ciencias 95:e20220596 PubMed37132750

2023

Nuclear Factor-Kappa B-induced miRNA-518a-5p represses trophoblast cell migration and invasion by the Nuclear Factor-Kappa B pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Xing Peng,Ruirui Zhang,Yumei Zhang,Chunyan Cai

PloS one 18:e0284875 PubMed37115777

2023

A standardized method for plasma extracellular vesicle isolation and size distribution analysis.

Applications

Unspecified application

Species

Unspecified reactive species

J Nathaniel Diehl,Amelia Ray,Lauren B Collins,Andrew Peterson,Kyle C Alexander,Jacob G Boutros,John S Ikonomidis,Adam W Akerman

FASEB journal : official publication of the Federation of American Societies for Experimental Biology 37:e22779 PubMed36723798

2023

ANP promotes HTR-8/SVneo cell invasion by upregulating protein kinase N 3 via autophagy inhibition.

Applications

Unspecified application

Species

Unspecified reactive species

Nan Chu,Yao Tang,Cheng-Jie Wang,Jiang-Nan Pei,Shou-Ling Luo,Yi Yu,Zhen-Zhen Liu,Hai-Yan Liu,Xue-Min Qiu,Ling Wang,Da-Jin Li,Wei-Rong Gu
View all publications
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