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AB53730

Anti-TIMP2 antibody

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(6 Publications)

Rabbit Polyclonal TIMP2 antibody. Suitable for WB and reacts with Human samples. Cited in 6 publications. Immunogen corresponding to Synthetic Peptide within Human TIMP2.

View Alternative Names

Metalloproteinase inhibitor 2, CSC-21K, Tissue inhibitor of metalloproteinases 2, TIMP-2, TIMP2

1 Images
Western blot - Anti-TIMP2 antibody (AB53730)
  • WB

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Western blot - Anti-TIMP2 antibody (AB53730)

All lanes:

Western blot - Anti-TIMP2 antibody (ab53730) at 1/500 dilution

Lane 1:

A549 cell extract

Lane 2:

A549 cell extract with immunising peptide

Predicted band size: 24 kDa

Observed band size: 31 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB

applications

Immunogen

Synthetic Peptide within Human TIMP2. The exact immunogen used to generate this antibody is proprietary information.

P16035

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
ab53730 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Storage information
Stable for 12 months at -20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

TIMP2 also known as Tissue Inhibitor of Metalloproteinases 2 mechanistically inhibits the activity of matrix metalloproteinases (MMPs). It binds to MMPs regulating the extracellular matrix's remodeling. The protein has a molecular mass of around 21 kDa. TIMP2 is expressed in various tissues including the brain liver and kidneys where it maintains a balance between matrix degradation and production.
Biological function summary

TIMP2 plays a significant role in tissue maintenance and repair by controlling extracellular matrix turnover. It forms complexes with MMPs such as MMP-2 inhibiting their proteolytic activities. The protein is also involved in cell proliferation and differentiation affecting processes like angiogenesis and wound healing. Its regulatory function aids in maintaining tissue homeostasis and preventing excessive matrix breakdown.

Pathways

TIMP2 is integral to the matrix metalloproteinase pathway and the regulation of endothelial cell migration. The protein interacts closely with other MMPs influencing their activation and modulation. Through these pathways TIMP2 holds an important position in cellular migration and signaling ensuring precise matrix remodeling in response to physiological needs.

TIMP2 has significant links with cancer and fibrosis. Dysregulation of TIMP2 expression or its imbalance with MMPs can contribute to tumor progression and metastasis due to uncontrolled matrix degradation. Additionally abnormal TIMP2 levels associate with fibrotic diseases where excessive tissue deposition occurs. TIMP2's interaction with proteins like MMP-9 and MMP-2 highlights its involvement in these pathological conditions.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Complexes with metalloproteinases (such as collagenases) and irreversibly inactivates them by binding to their catalytic zinc cofactor. Known to act on MMP-1, MMP-2, MMP-3, MMP-7, MMP-8, MMP-9, MMP-10, MMP-13, MMP-14, MMP-15, MMP-16 and MMP-19.
See full target information TIMP2
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Publications (6)

Recent publications for all applications. Explore the full list and refine your search

International journal of hypertension 2020:3123968 PubMed33381308

2020

p38/JNK Is Required for the Proliferation and Phenotype Changes of Vascular Smooth Muscle Cells Induced by in Essential Hypertension.

Applications

Unspecified application

Species

Unspecified reactive species

Chaowei Hu,Kun Zuo,Kuibao Li,Yuanfeng Gao,Mulei Chen,Roumu Hu,Ye Liu,Hongjie Chi,Hongjiang Wang,Yanwen Qin,Xiaoyan Liu,Jiuchang Zhong,Jun Cai,Xinchun Yang,Jing Li

Life science alliance 2: PubMed31160380

2019

Mesenchymal stromal cell activation by breast cancer secretomes in bioengineered 3D microenvironments.

Applications

Unspecified application

Species

Unspecified reactive species

Ulrich Blache,Edward R Horton,Tian Xia,Erwin M Schoof,Lene H Blicher,Angelina Schönenberger,Jess G Snedeker,Ivan Martin,Janine T Erler,Martin Ehrbar

PloS one 13:e0200704 PubMed30020979

2018

Collagen cross-linking impact on keratoconus extracellular matrix.

Applications

Unspecified application

Species

Unspecified reactive species

Rabab Sharif,Ben Fowler,Dimitrios Karamichos

FEBS letters 592:2499-2511 PubMed29933498

2018

A protein chimera strategy supports production of a model "difficult-to-express" recombinant target.

Applications

Unspecified application

Species

Unspecified reactive species

Hirra Hussain,David I Fisher,Robert G Roth,W Mark Abbott,Manuel Alejandro Carballo-Amador,Jim Warwicker,Alan J Dickson

Nature neuroscience 17:1655-7 PubMed25326689

2014

Synaptic plasticity mediating cocaine relapse requires matrix metalloproteinases.

Applications

IHC

Species

Mouse

Alexander C W Smith,Yonatan M Kupchik,Michael D Scofield,Cassandra D Gipson,Armina Wiggins,Charles A Thomas,Peter W Kalivas

International immunology 22:637-49 PubMed20501611

2010

MT6-MMP is present in lipid rafts and faces inward in living human PMNs but translocates to the cell surface during neutrophil apoptosis.

Applications

Unspecified application

Species

Unspecified reactive species

Carl F Fortin,Anjum Sohail,Qing Sun,Patrick P McDonald,Rafael Fridman,Tamàs Fülöp
View all publications
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