AB277794

Anti-TIMP3 antibody [20HCLC]

Recombinant
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(4 Publications)

Rabbit Recombinant Multiclonal TIMP3 antibody. Suitable for ICC, WB and reacts with Human, Mouse samples. Cited in 4 publications.

View Alternative Names

Metalloproteinase inhibitor 3, Protein MIG-5, Tissue inhibitor of metalloproteinases 3, TIMP-3, TIMP3

2 Images

Supplier Data

Immunocytochemistry - Anti-TIMP3 antibody [20HCLC] (AB277794)

Immunofluorescent analysis of TIMP3 in HeLa (human epithelial cell line from cervix adenocarcinoma) cells using a TIMP3 Recombinant Rabbit Multiclonal Antibody (ab277794) followed by detection using an Alexa Fluor^® 488-conjugated Goat anti-Rabbit secondary antibody (green) (Image A). Nuclei were stained using DAPI(Image B) and actin stained with Alexa Fluor^® 594 phalloidin (red) (image C). Image D is a composite image showing cytoplasmic localization of TIMP3.

Supplier Data

Western blot - Anti-TIMP3 antibody [20HCLC] (AB277794)

All lanes:

Western blot - Anti-TIMP3 antibody [20HCLC] (ab277794) at 2 µg/mL

All lanes:

Mouse C2C12 whole cell extracts

Predicted band size: 24 kDa

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Key facts

Host species

Rabbit

Clonality

Multiclonal

Clone number

20HCLC

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

WB, ICC

applications

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICC" : {"fullname" : "Immunocytochemistry", "shortname":"ICC"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICC-species-checked": "testedAndGuaranteed", "ICC-species-dilution-info": "1 µg/mL", "ICC-species-notes": "<p></p>", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Mouse": { "ICC-species-checked": "guaranteed", "ICC-species-dilution-info": "", "ICC-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "2-3 µg/mL", "WB-species-notes": "<p></p>" } } }

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Product details

What are recombinant multiclonals?
Recombinant multiclonals are a mixture of recombinant antibodies co-expressed from a library of heavy and light chains. They offer several advantages including:

- The sensitivity of polyclonal antibodies by recognising multiple epitopes
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

View our range of recombinant multiclonal antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

Preservative: 0.09% Sodium azide Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Tissue inhibitor of metalloproteinases 3 (TIMP3) is widely recognized for its role in inhibiting the activity of matrix metalloproteinases (MMPs). It has an approximate mass of 24 kDa and is often referred to by its alternate name MIG. TIMP3 is expressed in various tissues especially those involved in extracellular matrix composition such as the kidney and lungs. Its expression helps maintain the balance between matrix deposition and degradation which is critical for normal tissue homeostasis.

Biological function summary

The function of TIMP3 extends beyond mere inhibition of MMPs; it is also involved in regulating angiogenesis and apoptosis. TIMP3 forms complexes with extracellular matrix components acting as an anchor that modulates cell-matrix interactions. This capacity to bind to matrix elements enhances its inhibitory activity against proteases making it an important regulator of extracellular matrix integrity.

Pathways

TIMP3 plays significant roles in extracellular matrix remodeling and the inflammatory response. It participates in pathways involving structural organization and signaling mediation such as the NF-kB signaling cascade. TIMP3 regulates proteins like TNF-alpha and MMPs which are central to these pathways balancing inflammatory responses and tissue remodeling processes.

The imbalance of TIMP3 activity has been linked to chronic obstructive pulmonary disease (COPD) and age-related macular degeneration (AMD). In COPD impaired regulation of ECM turnover by TIMP3 leads to destructive lung tissue remodeling. In AMD the regulation of angiogenesis and degradation of ECM around the retina by TIMP3 is disturbed influencing disease progression. Its interaction with proteins such as VEGF is critical in these pathological contexts where altered TIMP3 expression or function can exacerbate disease states.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Complexes with metalloproteinases (such as collagenases) and irreversibly inactivates them by binding to their catalytic zinc cofactor. May form part of a tissue-specific acute response to remodeling stimuli. Known to act on MMP-1, MMP-2, MMP-3, MMP-7, MMP-9, MMP-13, MMP-14 and MMP-15.

See full target information TIMP3

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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Cellular and molecular neurobiology 44:33 PubMed38625414

2024

TIMP-3 Alleviates White Matter Injury After Subarachnoid Hemorrhage in Mice by Promoting Oligodendrocyte Precursor Cell Maturation.

Applications

Unspecified application

Species

Unspecified reactive species

Peiwen Guo,Xufang Ru,Jiru Zhou,Mao Chen,Yanling Li,Mingxu Duan,Yuanshu Li,Wenyan Li,Yujie Chen,Shilun Zuo,Hua Feng

Discover oncology 15:73 PubMed38478152

2024

GALNT2 targeted by miR-139-5p promotes proliferation of clear cell renal cell carcinoma via inhibition of LATS2 activation.

Applications

Unspecified application

Species

Unspecified reactive species

Haisheng Yi,Lingyun Liu,Jingshun Zhang,Kaimin Guo,Yin Cao,Penghao Sun,Hongliang Wang

Cell reports. Medicine 4:101228 PubMed37852176

2023

Replenishing decoy extracellular vesicles inhibits phenotype remodeling of tissue-resident cells in inflammation-driven arthritis.

Applications

Unspecified application

Species

Unspecified reactive species

Mengmeng Liang,Ke Wang,Xiaoyu Wei,Xiaoshan Gong,Hao Tang,Hao Xue,Jing Wang,Pengbin Yin,Licheng Zhang,Zaisong Ma,Ce Dou,Shiwu Dong,Jianzhong Xu,Fei Luo,Qinyu Ma

Frontiers in physiology 12:807747 PubMed35082694

2022

Tissue Inhibitor of Metalloproteinase-3 Ameliorates Diabetes-Induced Retinal Inflammation.

Applications

Unspecified application

Species

Unspecified reactive species

Ahmed M Abu El-Asrar,Ajmal Ahmad,Mohd Imtiaz Nawaz,Mohammad Mairaj Siddiquei,Alexandra De Zutter,Lotte Vanbrabant,Priscilla W Gikandi,Ghislain Opdenakker,Sofie Struyf

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-TIMP3 antibody [20HCLC]

Immunocytochemistry - Anti-TIMP3 antibody [20HCLC] (AB277794)

Western blot - Anti-TIMP3 antibody [20HCLC] (AB277794)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (4)

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