Rabbit Polyclonal TIMP3 antibody. Suitable for WB, ICC/IF and reacts with Human, Mouse samples. Cited in 72 publications. Immunogen corresponding to Synthetic Peptide within Human TIMP3.
Images
Publications
Filter by
- STAR protocols 3:1017582022Isolation, culture, and cryosectioning of primary porcine retinal pigment epithelium on transwell cell culture inserts.Applications:Unspecified applicationReactive species:Unspecified reactive speciesErika M S Hood,Christine A Curcio,Daniel LipinskiPubMed 36227744
- Regenerative biomaterials 9:rbac0742022Tannic acid-loaded hydrogel coating endues polypropylene mesh with hemostatic and anti-inflammatory capacity for facilitating pelvic floor repair.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChenghao Wu,Zixuan Zhou,Xi You,Yi Guo,Ping Chen,Huaifang Li,Xiaowen TongPubMed 36267152
- Oxidative medicine and cellular longevity 2022:93664942022Trehalose Protects Keratinocytes against Ultraviolet B Radiation by Activating Autophagy via Regulating TIMP3 and ATG9A.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLi Li,Hongying Chen,Xu Chen,Sihan Chen,Heng GuPubMed 35450405
- International journal of nanomedicine 17:1285-13072022Magnetic Nano-Platform Enhanced iPSC-Derived Trabecular Meshwork Delivery and Tracking Efficiency.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXiangji Wang,Qilong Cao,Shen Wu,Mohammad Reza Bahrani Fard,Ningli Wang,Jie Cao,Wei ZhuPubMed 35345785
- Cancer gene therapy 29:1355-13722022mA demethylase ALKBH5 promotes tumor cell proliferation by destabilizing IGF2BPs target genes and worsens the prognosis of patients with non-small-cell lung cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKazuo Tsuchiya,Katsuhiro Yoshimura,Yuji Iwashita,Yusuke Inoue,Tsutomu Ohta,Hirofumi Watanabe,Hidetaka Yamada,Akikazu Kawase,Masayuki Tanahashi,Hiroshi Ogawa,Kazuhito Funai,Kazuya Shinmura,Takafumi Suda,Haruhiko SugimuraPubMed 35318440
- Cancer research 82:1321-13392022Hotspot ESR1 Mutations Are Multimodal and Contextual Modulators of Breast Cancer Metastasis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZheqi Li,Yang Wu,Megan E Yates,Nilgun Tasdemir,Amir Bahreini,Jian Chen,Kevin M Levine,Nolan M Priedigkeit,Azadeh Nasrazadani,Simak Ali,Laki Buluwela,Spencer Arnesen,Jason Gertz,Jennifer K Richer,Benjamin Troness,Dorraya El-Ashry,Qiang Zhang,Lorenzo Gerratana,Youbin Zhang,Massimo Cristofanilli,Maritza A Montanez,Prithu Sundd,Callen T Wallace,Simon C Watkins,Caterina Fumagalli,Elena Guerini-Rocco,Li Zhu,George C Tseng,Nikhil Wagle,Jason S Carroll,Paul Jank,Carsten Denkert,Maria M Karsten,Jens-Uwe Blohmer,Ben H Park,Peter C Lucas,Jennifer M Atkinson,Adrian V Lee,Steffi OesterreichPubMed 35078818
- Frontiers in bioengineering and biotechnology 10:7730042022Oxo-M and 4-PPBP Delivery Multi-Domain Peptide Hydrogel Toward Tendon Regeneration.Applications:Unspecified applicationReactive species:Unspecified reactive speciesGa Young Park,Solaiman Tarafder,Samantha Lewis Eyen,Soomin Park,Ryunhyung Kim,Zain Siddiqui,Vivek Kumar,Chang H LeePubMed 35155388
- Gland surgery 10:3314-33232022MicroRNA-30d target TIMP3 induces pituitary tumor cell growth and invasion.Applications:Unspecified applicationReactive species:Unspecified reactive speciesBowen Sun,Congxin Dai,Shangfeng Zhao,Hao Dong,Haocheng Liu,Yong Li,Renzhi Wang,Jun KangPubMed 35070891
- Advanced healthcare materials 11:e21016192021In Situ Deployment of Engineered Extracellular Vesicles into the Tumor Niche via Myeloid-Derived Suppressor Cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSilvia Duarte-Sanmiguel,Ana Panic,Daniel J Dodd,Ana Salazar-Puerta,Jordan T Moore,William R Lawrence,Kylie Nairon,Carlie Francis,Natalie Zachariah,William McCoy,Rithvik Turaga,Aleksander Skardal,William E Carson,Natalia Higuita-Castro,Daniel Gallego-PerezPubMed 34662497
- Cell death & disease 12:8992021FKBP51 promotes invasion and migration by increasing the autophagic degradation of TIMP3 in clear cell renal cell carcinoma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesShaowei Mao,Di Zhang,Luan Chen,Jie Tan,Yunpeng Chu,Sijia Huang,Wenqi Zhou,Hengwei Qin,Qinghua Xia,Yueran Zhao,Rongxiu Li,Shengying Qin,Muyun WeiPubMed 34599146
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
Preservative: 0.05% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human TIMP3. The exact immunogen used to generate this antibody is proprietary information. Database link P35625
Consider this alternative
Reactivity data
Select an application| WB | ICC/IF | |
|---|---|---|
| Human | Tested | Tested |
| Mouse | Tested | Expected |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/1000 - 1/5000 | Notes Dilution optimised using Chromogenic detection. |
Species Mouse | Dilution info 1/1000 - 1/5000 | Notes Dilution optimised using Chromogenic detection. |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1-5 µg/mL | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1-5 µg/mL | Notes - |
Associated Products
Select an associated product type
7 products for Alternative Product
Target data
Function
Complexes with metalloproteinases (such as collagenases) and irreversibly inactivates them by binding to their catalytic zinc cofactor. May form part of a tissue-specific acute response to remodeling stimuli. Known to act on MMP-1, MMP-2, MMP-3, MMP-7, MMP-9, MMP-13, MMP-14 and MMP-15.
Alternative names
Metalloproteinase inhibitor 3, Protein MIG-5, Tissue inhibitor of metalloproteinases 3, TIMP-3, TIMP3
Recommended products
Rabbit Polyclonal TIMP3 antibody. Suitable for WB, ICC/IF and reacts with Human, Mouse samples. Cited in 72 publications. Immunogen corresponding to Synthetic Peptide within Human TIMP3.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
Preservative: 0.05% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human TIMP3. The exact immunogen used to generate this antibody is proprietary information. Database link P35625
- Purification technique
- Affinity purification Immunogen
- Specificity
ab39184 binds to TIMP3. It recognizes the glycosylated and unglycosylated forms of TIMP3, and works against native or reduced TIMP3. Ab39184 does not cross react with the other TIMP family members (TIMP1, TIMP2, TIMP4).
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Tissue inhibitor of metalloproteinases 3 (TIMP3) is widely recognized for its role in inhibiting the activity of matrix metalloproteinases (MMPs). It has an approximate mass of 24 kDa and is often referred to by its alternate name MIG. TIMP3 is expressed in various tissues especially those involved in extracellular matrix composition such as the kidney and lungs. Its expression helps maintain the balance between matrix deposition and degradation which is critical for normal tissue homeostasis.
- Biological function summary
The function of TIMP3 extends beyond mere inhibition of MMPs; it is also involved in regulating angiogenesis and apoptosis. TIMP3 forms complexes with extracellular matrix components acting as an anchor that modulates cell-matrix interactions. This capacity to bind to matrix elements enhances its inhibitory activity against proteases making it an important regulator of extracellular matrix integrity.
- Pathways
TIMP3 plays significant roles in extracellular matrix remodeling and the inflammatory response. It participates in pathways involving structural organization and signaling mediation such as the NF-kB signaling cascade. TIMP3 regulates proteins like TNF-alpha and MMPs which are central to these pathways balancing inflammatory responses and tissue remodeling processes.
- Associated diseases and disorders
The imbalance of TIMP3 activity has been linked to chronic obstructive pulmonary disease (COPD) and age-related macular degeneration (AMD). In COPD impaired regulation of ECM turnover by TIMP3 leads to destructive lung tissue remodeling. In AMD the regulation of angiogenesis and degradation of ECM around the retina by TIMP3 is disturbed influencing disease progression. Its interaction with proteins such as VEGF is critical in these pathological contexts where altered TIMP3 expression or function can exacerbate disease states.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
2 product images
Western blot - Anti-TIMP3 antibody (ab39184)
All lanes: Western blot - Anti-TIMP3 antibody (ab39184)
Lane 1: Human TIMP3
Lane 2: Mouse TIMP3
Lane 3: crude control (preparation of extracellular matrix from BHK cells producing recombinant human TIMP-3)
Predicted band size: 24 kDa
Observed band size: 21 kDa
Immunocytochemistry/ Immunofluorescence - Anti-TIMP3 antibody (ab39184)
ICC/IF image of ab39184 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab39184, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com