AB39184

Anti-TIMP3 antibody

Name: Anti-TIMP3 antibody (ab39184) | Abcam
Brand: Abcam Limited
SKU: AB39184
Price: 525 USD
Availability: InStock
Rating: 5 (5 reviews)

(5 Reviews)

(79 Publications)

Anti-TIMP3 antibody (ab39184) is a rabbit polyclonal antibody detecting TIMP3 in Western Blot, ICC/IF. Suitable for Human, Mouse.

- Over 70 publications
- Trusted since 2006

View Alternative Names

Metalloproteinase inhibitor 3, Protein MIG-5, Tissue inhibitor of metalloproteinases 3, TIMP-3, TIMP3

2 Images

Unknown

Western blot - Anti-TIMP3 antibody (AB39184)

All lanes:

Western blot - Anti-TIMP3 antibody (ab39184)

Lane 1:

Human TIMP3

Lane 2:

Mouse TIMP3

Lane 3:

crude control (preparation of extracellular matrix from BHK cells producing recombinant human TIMP-3)

Predicted band size: 24 kDa

Observed band size: 21 kDa

false

Immunocytochemistry/ Immunofluorescence - Anti-TIMP3 antibody (AB39184)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-TIMP3 antibody (AB39184)

ICC/IF image of ab39184 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab39184, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

ICC/IF, WB

applications

Immunogen

Synthetic Peptide within Human TIMP3. The exact immunogen used to generate this antibody is proprietary information.

P35625

Specificity

ab39184 binds to TIMP3. It recognizes the glycosylated and unglycosylated forms of TIMP3, and works against native or reduced TIMP3. Ab39184 does not cross react with the other TIMP family members (TIMP1, TIMP2, TIMP4).

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "Dilution optimised using Chromogenic detection.", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1-5 µg/mL", "ICCIF-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "Dilution optimised using Chromogenic detection.", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "1-5 µg/mL", "ICCIF-species-notes": "" } } }

style

left-column

Product details

What is this antibody validated in?
Anti-TIMP3 antibody (ab39184) is a rabbit polyclonal antibody and is validated for use in Western Blot (WB), Immunocytochemistry/immunofluorescence (ICC/IF) in Human, Mouse samples.

What is the molecular weight of TIMP3?
Anti-TIMP3 (ab39184) specifically detects a band for TIMP3 (UniProt: P35625) at a molecular weight of 24kDa.

Trusted by the scientific community
Anti-TIMP3 (ab39184) was first used in a scientific publication in 2006 and has been cited over 70 times in peer-reviewed journals.

Reviewed by scientists
Anti-TIMP3 (ab39184) has over 5 independent reviews from customers.

style

left-column

Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.4 Preservative: 0.05% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Tissue inhibitor of metalloproteinases 3 (TIMP3) is widely recognized for its role in inhibiting the activity of matrix metalloproteinases (MMPs). It has an approximate mass of 24 kDa and is often referred to by its alternate name MIG. TIMP3 is expressed in various tissues especially those involved in extracellular matrix composition such as the kidney and lungs. Its expression helps maintain the balance between matrix deposition and degradation which is critical for normal tissue homeostasis.

Biological function summary

The function of TIMP3 extends beyond mere inhibition of MMPs; it is also involved in regulating angiogenesis and apoptosis. TIMP3 forms complexes with extracellular matrix components acting as an anchor that modulates cell-matrix interactions. This capacity to bind to matrix elements enhances its inhibitory activity against proteases making it an important regulator of extracellular matrix integrity.

Pathways

TIMP3 plays significant roles in extracellular matrix remodeling and the inflammatory response. It participates in pathways involving structural organization and signaling mediation such as the NF-kB signaling cascade. TIMP3 regulates proteins like TNF-alpha and MMPs which are central to these pathways balancing inflammatory responses and tissue remodeling processes.

The imbalance of TIMP3 activity has been linked to chronic obstructive pulmonary disease (COPD) and age-related macular degeneration (AMD). In COPD impaired regulation of ECM turnover by TIMP3 leads to destructive lung tissue remodeling. In AMD the regulation of angiogenesis and degradation of ECM around the retina by TIMP3 is disturbed influencing disease progression. Its interaction with proteins such as VEGF is critical in these pathological contexts where altered TIMP3 expression or function can exacerbate disease states.

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

Complexes with metalloproteinases (such as collagenases) and irreversibly inactivates them by binding to their catalytic zinc cofactor. May form part of a tissue-specific acute response to remodeling stimuli. Known to act on MMP-1, MMP-2, MMP-3, MMP-7, MMP-9, MMP-13, MMP-14 and MMP-15.

See full target information TIMP3

style

left-column

Publications (79)

Recent publications for all applications. Explore the full list and refine your search

Nature cardiovascular research 4:1066-1076 PubMed40866626

2025

Human ocular fluid outflow on-chip reveals trabecular meshwork-mediated Schlemm's canal endothelial dysfunction in steroid-induced glaucoma.

Applications

Unspecified application

Species

Unspecified reactive species

Renhao Lu,Anna M Kolarzyk,W Daniel Stamer,Esak Lee

STAR protocols 3:101758 PubMed36227744

2022

Isolation, culture, and cryosectioning of primary porcine retinal pigment epithelium on transwell cell culture inserts.

Applications

Unspecified application

Species

Unspecified reactive species

Erika M S Hood,Christine A Curcio,Daniel Lipinski

Regenerative biomaterials 9:rbac074 PubMed36267152

2022

Tannic acid-loaded hydrogel coating endues polypropylene mesh with hemostatic and anti-inflammatory capacity for facilitating pelvic floor repair.

Applications

Unspecified application

Species

Unspecified reactive species

Chenghao Wu,Zixuan Zhou,Xi You,Yi Guo,Ping Chen,Huaifang Li,Xiaowen Tong

Journal of food and drug analysis 30:479-492 PubMed39666293

2022

Diallyl disulfide inhibits proliferation, epithelial-mesenchymal transition, and invasion through RORα-mediated downregulation of Wnt1/β-catenin pathway in gastric cancer cells.

Applications

Unspecified application

Species

Unspecified reactive species

Jian Su,Hui He,Yu-Kun Li,Hong Xia,Fang Liu,Ying Zeng,Xi Zeng,Hui Ling,Bo Su,Qi Su

Journal of inflammation research 15:4693-4708 PubMed35996686

2022

Ezh2 Inhibits Replicative Senescence of Atrial Fibroblasts Through Promotion of H3K27me3 in the Promoter Regions of CDKN2a and Timp4 Genes.

Applications

Unspecified application

Species

Unspecified reactive species

Yingze Li,Guojian Fang,Wei Cao,Jiali Yuan,Shuai Song,Hong Peng,Yuepeng Wang,Qunshan Wang

Oxidative medicine and cellular longevity 2022:9366494 PubMed35450405

2022

Trehalose Protects Keratinocytes against Ultraviolet B Radiation by Activating Autophagy via Regulating TIMP3 and ATG9A.

Applications

Unspecified application

Species

Unspecified reactive species

Li Li,Hongying Chen,Xu Chen,Sihan Chen,Heng Gu

International journal of nanomedicine 17:1285-1307 PubMed35345785

2022

Magnetic Nano-Platform Enhanced iPSC-Derived Trabecular Meshwork Delivery and Tracking Efficiency.

Applications

Unspecified application

Species

Unspecified reactive species

Xiangji Wang,Qilong Cao,Shen Wu,Mohammad Reza Bahrani Fard,Ningli Wang,Jie Cao,Wei Zhu

Cancer gene therapy 29:1355-1372 PubMed35318440

2022

mA demethylase ALKBH5 promotes tumor cell proliferation by destabilizing IGF2BPs target genes and worsens the prognosis of patients with non-small-cell lung cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Kazuo Tsuchiya,Katsuhiro Yoshimura,Yuji Iwashita,Yusuke Inoue,Tsutomu Ohta,Hirofumi Watanabe,Hidetaka Yamada,Akikazu Kawase,Masayuki Tanahashi,Hiroshi Ogawa,Kazuhito Funai,Kazuya Shinmura,Takafumi Suda,Haruhiko Sugimura

Frontiers in bioengineering and biotechnology 10:773004 PubMed35155388

2022

Oxo-M and 4-PPBP Delivery Multi-Domain Peptide Hydrogel Toward Tendon Regeneration.

Applications

Unspecified application

Species

Unspecified reactive species

Ga Young Park,Solaiman Tarafder,Samantha Lewis Eyen,Soomin Park,Ryunhyung Kim,Zain Siddiqui,Vivek Kumar,Chang H Lee

Cancer research 82:1321-1339 PubMed35078818

2022

Hotspot ESR1 Mutations Are Multimodal and Contextual Modulators of Breast Cancer Metastasis.

Applications

Unspecified application

Species

Unspecified reactive species

Zheqi Li,Yang Wu,Megan E Yates,Nilgun Tasdemir,Amir Bahreini,Jian Chen,Kevin M Levine,Nolan M Priedigkeit,Azadeh Nasrazadani,Simak Ali,Laki Buluwela,Spencer Arnesen,Jason Gertz,Jennifer K Richer,Benjamin Troness,Dorraya El-Ashry,Qiang Zhang,Lorenzo Gerratana,Youbin Zhang,Massimo Cristofanilli,Maritza A Montanez,Prithu Sundd,Callen T Wallace,Simon C Watkins,Caterina Fumagalli,Elena Guerini-Rocco,Li Zhu,George C Tseng,Nikhil Wagle,Jason S Carroll,Paul Jank,Carsten Denkert,Maria M Karsten,Jens-Uwe Blohmer,Ben H Park,Peter C Lucas,Jennifer M Atkinson,Adrian V Lee,Steffi Oesterreich

View all publications

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-TIMP3 antibody

Western blot - Anti-TIMP3 antibody (AB39184)

Immunocytochemistry/ Immunofluorescence - Anti-TIMP3 antibody (AB39184)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (79)

Alternative Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Complementary Products

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Product promise