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AB276134

Anti-TIMP3 antibody [EPR23943-149]

3

(1 Review)

|

(8 Publications)

Rabbit Recombinant Monoclonal TIMP3 antibody. Suitable for ICC/IF, WB, Flow Cyt (Intra) and reacts with Human samples. Cited in 8 publications.

View Alternative Names

Metalloproteinase inhibitor 3, Protein MIG-5, Tissue inhibitor of metalloproteinases 3, TIMP-3, TIMP3

6 Images
Immunocytochemistry/ Immunofluorescence - Anti-TIMP3 antibody [EPR23943-149] (AB276134)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-TIMP3 antibody [EPR23943-149] (AB276134)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized MDA-MB-231 cells labelling TIMP3 with ab276134 at 1/50 (9.16 ug/ml) dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). Confocal image showing cytoplasmic staining in MDA-MB-231 cells is observed. ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue).

Secondary antibody only control : Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.

Flow Cytometry (Intracellular) - Anti-TIMP3 antibody [EPR23943-149] (AB276134)
  • Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-TIMP3 antibody [EPR23943-149] (AB276134)

Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol-permeabilized MDA-MB-231 (human breast adenocarcinoma epithelial cell) cells labelling TIMP3 with ab276134 at 1/500 dilution (0.1ug) (Red) compared with a Rabbit monoclonal IgG (ab172730) isotype control (Black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).

A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.

Western blot - Anti-TIMP3 antibody [EPR23943-149] (AB276134)
  • WB

Lab

Western blot - Anti-TIMP3 antibody [EPR23943-149] (AB276134)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID : 8174111, 30894640).

Low expression tissue : human liver (PMID : 8174111).

Exposure time : 125 seconds.

All lanes:

Western blot - Anti-TIMP3 antibody [EPR23943-149] (ab276134) at 1/1000 dilution

Lane 1:

Human placenta tissue lysate at 20 µg

Lane 2:

Human liver tissue lysate at 20 µg

Secondary

All lanes:

Western blot - VeriBlot for IP Detection Reagent (HRP) (<a href='/en-us/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/1000 dilution

Predicted band size: 24 kDa

Observed band size: 20 kDa

false

Western blot - Anti-TIMP3 antibody [EPR23943-149] (AB276134)
  • WB

Lab

Western blot - Anti-TIMP3 antibody [EPR23943-149] (AB276134)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Exposure time : 1 second.

All lanes:

Western blot - Anti-TIMP3 antibody [EPR23943-149] (ab276134) at 1/1000 dilution

All lanes:

Recombinant Human TIMP-3 Protein, 20ng

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/50000 dilution

Predicted band size: 24 kDa

Observed band size: 25 kDa

false

Western blot - Anti-TIMP3 antibody [EPR23943-149] (AB276134)
  • WB

Lab

Western blot - Anti-TIMP3 antibody [EPR23943-149] (AB276134)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID : 8174111, 30894640).

Exposure time : 3 minutes.

All lanes:

Western blot - Anti-TIMP3 antibody [EPR23943-149] (ab276134) at 1/1000 dilution

All lanes:

MDA-MB-231 (human breast adenocarcinoma epithelial cell) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/50000 dilution

Predicted band size: 24 kDa

Observed band size: 20 kDa

false

Western blot - Anti-TIMP3 antibody [EPR23943-149] (AB276134)
  • WB

Lab

Western blot - Anti-TIMP3 antibody [EPR23943-149] (AB276134)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID : 8174111, 30894640).

Exposure time : 92 seconds.

All lanes:

Western blot - Anti-TIMP3 antibody [EPR23943-149] (ab276134) at 1/1000 dilution

All lanes:

Human lung tissue lysate at 20 µg

Secondary

All lanes:

Western blot - VeriBlot for IP Detection Reagent (HRP) (<a href='/en-us/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/1000 dilution

Predicted band size: 24 kDa

Observed band size: 20 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR23943-149

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

Flow Cyt (Intra), ICC/IF, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50", "ICCIF-species-notes": "<p></p>", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/500", "FlowCytIntra-species-notes": "<p></p>" }, "Mouse": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Tissue inhibitor of metalloproteinases 3 (TIMP3) is widely recognized for its role in inhibiting the activity of matrix metalloproteinases (MMPs). It has an approximate mass of 24 kDa and is often referred to by its alternate name MIG. TIMP3 is expressed in various tissues especially those involved in extracellular matrix composition such as the kidney and lungs. Its expression helps maintain the balance between matrix deposition and degradation which is critical for normal tissue homeostasis.
Biological function summary

The function of TIMP3 extends beyond mere inhibition of MMPs; it is also involved in regulating angiogenesis and apoptosis. TIMP3 forms complexes with extracellular matrix components acting as an anchor that modulates cell-matrix interactions. This capacity to bind to matrix elements enhances its inhibitory activity against proteases making it an important regulator of extracellular matrix integrity.

Pathways

TIMP3 plays significant roles in extracellular matrix remodeling and the inflammatory response. It participates in pathways involving structural organization and signaling mediation such as the NF-kB signaling cascade. TIMP3 regulates proteins like TNF-alpha and MMPs which are central to these pathways balancing inflammatory responses and tissue remodeling processes.

The imbalance of TIMP3 activity has been linked to chronic obstructive pulmonary disease (COPD) and age-related macular degeneration (AMD). In COPD impaired regulation of ECM turnover by TIMP3 leads to destructive lung tissue remodeling. In AMD the regulation of angiogenesis and degradation of ECM around the retina by TIMP3 is disturbed influencing disease progression. Its interaction with proteins such as VEGF is critical in these pathological contexts where altered TIMP3 expression or function can exacerbate disease states.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Complexes with metalloproteinases (such as collagenases) and irreversibly inactivates them by binding to their catalytic zinc cofactor. May form part of a tissue-specific acute response to remodeling stimuli. Known to act on MMP-1, MMP-2, MMP-3, MMP-7, MMP-9, MMP-13, MMP-14 and MMP-15.
See full target information TIMP3
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Publications (8)

Recent publications for all applications. Explore the full list and refine your search

Materials today. Bio 34:102234 PubMed40893347

2025

Cationic microbubble loading hSIRT3 and hTIMP3 optimize cardiac-targeted delivery and myocardial protection in the porcine MI/R model.

Applications

Unspecified application

Species

Unspecified reactive species

Peian Cai,Kegong Chen,Xionghai Qin,Xingpei Jiang,Xuan Jiao,Kexun Liu,Erliang Guo,Zipeng Li,Xianxin Qiu,Chang Liu,Lu Sun,Junbo Chuai,Jie Wu,Wei Chen,Hai Tian

Frontiers in cardiovascular medicine 10:1250234 PubMed37655218

2023

Manipulation of components of the renin angiotensin system in renal proximal tubules fails to alter atherosclerosis in hypercholesterolemic mice.

Applications

Unspecified application

Species

Unspecified reactive species

Masayoshi Kukida,Naofumi Amioka,Dien Ye,Hui Chen,Jessica J Moorleghen,Ching-Ling Liang,Deborah A Howatt,Yuriko Katsumata,Motoko Yanagita,Hisashi Sawada,Alan Daugherty,Hong S Lu

Molecular metabolism 74:101746 PubMed37286033

2023

Mapping the transcriptional landscape of human white and brown adipogenesis using single-nuclei RNA-seq.

Applications

Unspecified application

Species

Unspecified reactive species

Anushka Gupta,Vissarion Efthymiou,Sean D Kodani,Farnaz Shamsi,Mary Elizabeth Patti,Yu-Hua Tseng,Aaron Streets

Life science alliance 6: PubMed37037595

2023

EZH2 controls epicardial cell migration during heart development.

Applications

Unspecified application

Species

Unspecified reactive species

Haobin Jiang,Lina Bai,Shen Song,Qianqian Yin,Anteng Shi,Bin Zhou,Hong Lian,Houzao Chen,Cheng-Ran Xu,Yanchun Wang,Yu Nie,Shengshou Hu

International journal of experimental pathology 103:44-53 PubMed35156240

2022

Remifentanil reduces the proliferation, migration and invasion of HCC cells via lncRNA NBR2/miR-650/TIMP3 axis.

Applications

Unspecified application

Species

Unspecified reactive species

Wei Liang,Jinyuan Ke

Oxidative medicine and cellular longevity 2022:2785113 PubMed35116091

2022

Exosomal miR-17-3p Alleviates Programmed Necrosis in Cardiac Ischemia/Reperfusion Injury by Regulating TIMP3 Expression.

Applications

Unspecified application

Species

Unspecified reactive species

Zhuyuan Liu,Didi Zhu,Fuchao Yu,Mingming Yang,Dan Huang,Zhenjun Ji,Wenbin Lu,Genshan Ma

Diabetology & metabolic syndrome 13:89 PubMed34446088

2021

Long non-coding RNA CASC2 restrains high glucose-induced proliferation, inflammation and fibrosis in human glomerular mesangial cells through mediating miR-135a-5p/TIMP3 axis and JNK signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Dongju Zhu,Xiang Wu,Qian Xue

International journal of general medicine 14:27-39 PubMed33447074

2021

MicroRNA-21 Contributes to Cutaneous Squamous Cell Carcinoma Progression via Mediating TIMP3/PI3K/AKT Signaling Axis.

Applications

Unspecified application

Species

Unspecified reactive species

Shuhong Yin,Xiuying Lin
View all publications
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Product promise

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