JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB276139

Anti-TIMP3 antibody [EPR23943-149] - BSA and Azide free

Be the first to review this product! Submit a review

|

(0 Publication)

Rabbit Recombinant Monoclonal TIMP3 antibody. Carrier free. Suitable for ICC/IF, WB, Flow Cyt (Intra) and reacts with Human samples.

View Alternative Names

Metalloproteinase inhibitor 3, Protein MIG-5, Tissue inhibitor of metalloproteinases 3, TIMP-3, TIMP3

6 Images
Flow Cytometry (Intracellular) - Anti-TIMP3 antibody [EPR23943-149] - BSA and Azide free (AB276139)
  • Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-TIMP3 antibody [EPR23943-149] - BSA and Azide free (AB276139)

This data was developed using ab276134, the same antibody clone in a different buffer formulation.

Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol-permeabilized MDA-MB-231 (human breast adenocarcinoma epithelial cell) cells labelling TIMP3 with ab276134 at 1/500 dilution (0.1ug) (Red) compared with a Rabbit monoclonal IgG (ab172730) isotype control (Black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).

A Goat anti rabbit IgG (Alexa Fluor®488, ab150077) at 1/2000 dilution was used as the secondary antibody.

Western blot - Anti-TIMP3 antibody [EPR23943-149] - BSA and Azide free (AB276139)
  • WB

Lab

Western blot - Anti-TIMP3 antibody [EPR23943-149] - BSA and Azide free (AB276139)

This data was developed using ab276134, the same antibody clone in a different buffer formulation. Blocking and diluting buffer and concentration : 5% NFDM/TBST. The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID : 8174111, 30894640). **Low expression tissue : ** human liver (PMID : 8174111). Exposure time : 125 seconds.

All lanes:

Western blot - Anti-TIMP3 antibody [EPR23943-149] (<a href='/en-us/products/primary-antibodies/timp3-antibody-epr23943-149-ab276134'>ab276134</a>) at 1/1000 dilution

Lane 1:

Human placenta tissue lysate at 20 µg

Lane 2:

Human liver tissue lysate at 20 µg

Secondary

All lanes:

Western blot - VeriBlot for IP Detection Reagent (HRP) (<a href='/en-us/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/1000 dilution

Predicted band size: 24 kDa

Observed band size: 20 kDa

false

Immunocytochemistry/ Immunofluorescence - Anti-TIMP3 antibody [EPR23943-149] - BSA and Azide free (AB276139)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-TIMP3 antibody [EPR23943-149] - BSA and Azide free (AB276139)

This data was developed using ab276134, the same antibody clone in a different buffer formulation.

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized MDA-MB-231 cells labelling TIMP3 with ab276134 at 1/50 (9.16 ug/ml) dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). Confocal image showing cytoplasmic staining in MDA-MB-231 cells is observed. ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue).

Secondary antibody only control : Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.

Western blot - Anti-TIMP3 antibody [EPR23943-149] - BSA and Azide free (AB276139)
  • WB

Lab

Western blot - Anti-TIMP3 antibody [EPR23943-149] - BSA and Azide free (AB276139)

This data was developed using ab276134, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration :

1 second

Exposure time :

All lanes:

Western blot - Anti-TIMP3 antibody [EPR23943-149] (<a href='/en-us/products/primary-antibodies/timp3-antibody-epr23943-149-ab276134'>ab276134</a>) at 1/1000 dilution

All lanes:

Recombinant Human TIMP-3 Protein, 20ng

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/50000 dilution

Predicted band size: 24 kDa

Observed band size: 25 kDa

false

Western blot - Anti-TIMP3 antibody [EPR23943-149] - BSA and Azide free (AB276139)
  • WB

Lab

Western blot - Anti-TIMP3 antibody [EPR23943-149] - BSA and Azide free (AB276139)

This data was developed using ab276134, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID : 8174111, 30894640).

Exposure time : 3 minutes.

All lanes:

Western blot - Anti-TIMP3 antibody [EPR23943-149] (<a href='/en-us/products/primary-antibodies/timp3-antibody-epr23943-149-ab276134'>ab276134</a>) at 1/1000 dilution

All lanes:

MDA-MB-231 (human breast adenocarcinoma epithelial cell) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/50000 dilution

Predicted band size: 24 kDa

Observed band size: 20 kDa

false

Western blot - Anti-TIMP3 antibody [EPR23943-149] - BSA and Azide free (AB276139)
  • WB

Lab

Western blot - Anti-TIMP3 antibody [EPR23943-149] - BSA and Azide free (AB276139)

This data was developed using ab276134, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID : 8174111, 30894640).

Exposure time : 92 seconds.

All lanes:

Western blot - Anti-TIMP3 antibody [EPR23943-149] (<a href='/en-us/products/primary-antibodies/timp3-antibody-epr23943-149-ab276134'>ab276134</a>) at 1/1000 dilution

All lanes:

Human lung tissue lysate at 20 µg

Secondary

All lanes:

Western blot - VeriBlot for IP Detection Reagent (HRP) (<a href='/en-us/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/1000 dilution

Predicted band size: 24 kDa

Observed band size: 20 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR23943-149

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

Flow Cyt (Intra), ICC/IF, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>" }, "Mouse": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }
style
left-column

Product details

ab276139 is the carrier-free version of ab276134.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
Constituents: 100% PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Tissue inhibitor of metalloproteinases 3 (TIMP3) is widely recognized for its role in inhibiting the activity of matrix metalloproteinases (MMPs). It has an approximate mass of 24 kDa and is often referred to by its alternate name MIG. TIMP3 is expressed in various tissues especially those involved in extracellular matrix composition such as the kidney and lungs. Its expression helps maintain the balance between matrix deposition and degradation which is critical for normal tissue homeostasis.
Biological function summary

The function of TIMP3 extends beyond mere inhibition of MMPs; it is also involved in regulating angiogenesis and apoptosis. TIMP3 forms complexes with extracellular matrix components acting as an anchor that modulates cell-matrix interactions. This capacity to bind to matrix elements enhances its inhibitory activity against proteases making it an important regulator of extracellular matrix integrity.

Pathways

TIMP3 plays significant roles in extracellular matrix remodeling and the inflammatory response. It participates in pathways involving structural organization and signaling mediation such as the NF-kB signaling cascade. TIMP3 regulates proteins like TNF-alpha and MMPs which are central to these pathways balancing inflammatory responses and tissue remodeling processes.

The imbalance of TIMP3 activity has been linked to chronic obstructive pulmonary disease (COPD) and age-related macular degeneration (AMD). In COPD impaired regulation of ECM turnover by TIMP3 leads to destructive lung tissue remodeling. In AMD the regulation of angiogenesis and degradation of ECM around the retina by TIMP3 is disturbed influencing disease progression. Its interaction with proteins such as VEGF is critical in these pathological contexts where altered TIMP3 expression or function can exacerbate disease states.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Complexes with metalloproteinases (such as collagenases) and irreversibly inactivates them by binding to their catalytic zinc cofactor. May form part of a tissue-specific acute response to remodeling stimuli. Known to act on MMP-1, MMP-2, MMP-3, MMP-7, MMP-9, MMP-13, MMP-14 and MMP-15.
See full target information TIMP3
style
left-column
style
left-column
style
right-column

Complementary Products

Assay Kits

AB119608

Human TIMP3 ELISA Kit (MIG-5)

Sandwich ELISA - Human TIMP3 ELISA Kit (MIG-5) (AB119608)

  • 0
  • 0
Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Primary Antibodies

AB1828

Anti-TIMP2 antibody [3A4]

BOND RX™ Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TIMP2 antibody [3A4] (AB1828)

  • 4
  • 7
Primary Antibodies

AB92536

Anti-MMP2 antibody [EPR1184]

RabMAb|Recombinant

Western blot - Anti-MMP2 antibody [EPR1184] (AB92536)

  • 4
  • 6
Primary Antibodies

AB7817

Anti-alpha smooth muscle Actin antibody [1A4]

KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha smooth muscle Actin antibody [1A4] (AB7817)

  • 5
  • 105

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com