AB58425

Anti-TIMP4 antibody

Name: Anti-TIMP4 antibody (ab58425) | Abcam
Brand: Abcam Limited
SKU: AB58425

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(15 Publications)

Rabbit Polyclonal TIMP4 antibody. Suitable for ELISA, WB, IHC-P, IHC-Fr and reacts with Mouse, Rat, Human samples. Cited in 15 publications. Immunogen corresponding to Synthetic Peptide within Human Metalloproteinase inhibitor 4.

View Alternative Names

Metalloproteinase inhibitor 4, Tissue inhibitor of metalloproteinases 4, TIMP-4, TIMP4

2 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TIMP4 antibody (AB58425)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TIMP4 antibody (AB58425)

ab58425, at 1/50 dilution, staining TIMP4 in paraffin embedded human lung carcinoma tissue by Immunohistochemistry, in the absence (left image) or presence (right image) of the immunising peptide.

Unknown

Western blot - Anti-TIMP4 antibody (AB58425)

All lanes:

Western blot - Anti-TIMP4 antibody (ab58425) at 1/500 dilution

Lane 1:

NIH3T3 cell extract

Lane 2:

NIH3T3 cell extract with immunising peptide

Predicted band size: 25 kDa

Observed band size: 26 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human, Rat

Applications

IHC-Fr, ELISA, WB, IHC-P

applications

Immunogen

Synthetic Peptide within Human Metalloproteinase inhibitor 4. The exact immunogen used to generate this antibody is proprietary information.

Q99727

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ELISA" : {"fullname" : "ELISA", "shortname":"ELISA"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" }, "Mouse": { "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" }, "Rat": { "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

ab58425 was affinity purified from rabbit antiserum by affinity chromatography using epitope specific immunogen.

Storage buffer

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Stable for 12 months at -20°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Tissue Inhibitor of Metalloproteinases 4 (TIMP4) a member of the TIMP family plays a mechanical role by inhibiting matrix metalloproteinases (MMPs). This inhibition helps regulate extracellular matrix (ECM) remodeling. TIMP4 weighs approximately 22 kDa and is expressed in various tissues including the heart brain and reproductive organs. It binds directly to MMPs forming stable complexes that prevent the degradation of ECM components.

Biological function summary

TIMP4 contributes to maintaining tissue homeostasis and integrity. By regulating ECM degradation it influences processes such as cell migration proliferation and angiogenesis. TIMP4 does not work alone; it associates with enzymes like MMP-2 and MMP-14 modulating their activities to keep ECM dynamics in check. This regulation is essential for normal physiological functions and also during tissue repair.

Pathways

TIMP4 participates in the balance between ECM synthesis and degradation. It takes part in the ECM receptor interaction and focal adhesion pathways. These pathways involve several proteins including integrins and collagens which interact with ECM components modulated by TIMP4 activity. TIMP4 helps coordinate cellular responses to environmental changes through these pathways thereby influencing cell behavior and tissue organization.

TIMP4 has associations with cardiovascular diseases and cancer. In cardiac contexts altered TIMP4 levels impact heart function and remodeling often linked to cardiovascular diseases. Its dysregulation can contribute to tumor progression in cancers by affecting ECM degradation and subsequent metastasis. TIMP4's interaction with proteins like MMP-9 links it to both tumor invasiveness and vascular remodeling in these disease states.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Complexes with metalloproteinases (such as collagenases) and irreversibly inactivates them by binding to their catalytic zinc cofactor. Known to act on MMP-1, MMP-2, MMP-3, MMP-7 and MMP-9.

See full target information Metalloproteinase inhibitor 4

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Publications (15)

Recent publications for all applications. Explore the full list and refine your search

Cell communication and signaling : CCS 21:199 PubMed37563688

2023

N6-methyladenosine (m6A) methyltransferase WTAP-mediated miR-92b-5p accelerates osteoarthritis progression.

Applications

Unspecified application

Species

Unspecified reactive species

Zhaowei Lin,Tao Jiang,Wei Zheng,Jiayuan Zhang,Anan Li,Chao Lu,Wengang Liu

Heart and vessels 38:122-130 PubMed36070095

2022

Cellular-scale sex differences in extracellular matrix remodeling by valvular interstitial cells.

Applications

Unspecified application

Species

Unspecified reactive species

LaTonya R Simon,Ashley J Scott,Lysmarie Figueroa Rios,Joshua Zembles,Kristyn S Masters

Annals of biomedical engineering 49:1909-1922 PubMed33768411

2021

Quantitative Morphometry of Elastic Fibers in Pelvic Organ Prolapse.

Applications

Unspecified application

Species

Unspecified reactive species

Shataakshi Dahal,Mei Kuang,Anna Rietsch,R S Butler,Anand Ramamurthi,Margot S Damaser

Physiological reports 8:e14436 PubMed32533648

2020

Elastin homeostasis is altered with pelvic organ prolapse in cultures of vaginal cells from a lysyl oxidase-like 1 knockout mouse model.

Applications

Unspecified application

Species

Unspecified reactive species

Slater A Jameson,Ganesh Swaminathan,Shataakshi Dahal,Bruna Couri,Mei Kuang,Anna Rietsch,Robert S Butler,Anand Ramamurthi,Margot S Damaser

Cardiovascular research 117:188-200 PubMed31995179

2020

MMP inhibitors attenuate doxorubicin cardiotoxicity by preventing intracellular and extracellular matrix remodelling.

Applications

Unspecified application

Species

Unspecified reactive species

Brandon Y H Chan,Andrej Roczkowsky,Woo Jung Cho,Mathieu Poirier,Consolato Sergi,Vic Keschrumrus,Jared M Churko,Henk Granzier,Richard Schulz

Journal of the American Heart Association 7:e010427 PubMed30371322

2018

Disparate Remodeling of the Extracellular Matrix and Proteoglycans in Failing Pediatric Versus Adult Hearts.

Applications

Unspecified application

Species

Unspecified reactive species

Sayantan Jana,Hao Zhang,Gary D Lopaschuk,Darren H Freed,Consolato Sergi,Paul F Kantor,Gavin Y Oudit,Zamaneh Kassiri

American journal of physiology. Heart and circulat 315:H463-H473 PubMed29775412

2018

Inhibitor of lysyl oxidase improves cardiac function and the collagen/MMP profile in response to volume overload.

Applications

Unspecified application

Species

Unspecified reactive species

Elia C El Hajj,Milad C El Hajj,Van K Ninh,Jason D Gardner

International journal of chronic obstructive pulmo 12:717-724 PubMed28260878

2017

Effect of simvastatin on MMPs and TIMPs in cigarette smoke-induced rat COPD model.

Applications

IHC-P

Species

Rat

Jiawei Sun,Jie Bao,Yanan Shi,Bin Zhang,Lindong Yuan,Junhong Li,Lihai Zhang,Mo Sun,Ling Zhang,Wuzhuang Sun

Journal of cellular and molecular medicine 20:2089-2101 PubMed27396717

2016

Epigenetic silencing of TIMP4 in heart failure.

Applications

Species

Unspecified reactive species

Pankaj Chaturvedi,Suresh C Tyagi

Investigative ophthalmology & visual science 56:8392-402 PubMed26747770

2016

Lacrimal Gland Inflammation Deregulates Extracellular Matrix Remodeling and Alters Molecular Signature of Epithelial Stem/Progenitor Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Takeshi Umazume,William M Thomas,Sabrina Campbell,Hema Aluri,Suharika Thotakura,Driss Zoukhri,Helen P Makarenkova

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-TIMP4 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TIMP4 antibody (AB58425)

Western blot - Anti-TIMP4 antibody (AB58425)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Assay Kits

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (15)

Product promise