AB285049

Anti-TIMP4 antibody [EPR22830-311] - BSA and Azide free (Capture)

RabMAb
Recombinant
What is this?

Be the first to review this product! Submit a review

(0 Publication)

Rabbit Recombinant Monoclonal TIMP4 antibody. Carrier free. Suitable for sELISA and reacts with Human samples.

View Alternative Names

Metalloproteinase inhibitor 4, Tissue inhibitor of metalloproteinases 4, TIMP-4, TIMP4

1 Images

Sandwich ELISA - Anti-TIMP4 antibody [EPR22830-311] - BSA and Azide free (Capture) (AB285049)

sELISA

Lab

Sandwich ELISA - Anti-TIMP4 antibody [EPR22830-311] - BSA and Azide free (Capture) (AB285049)

Sandwich ELISA of ab253883 with the capture antibody ab285049 dilution at 2 µg/mL and detector antibody ab285155 dilution at 0.5 µg/mL.

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR22830-311

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

sELISA

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "sELISA" : {"fullname" : "Sandwich ELISA", "shortname":"sELISA"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "sELISA-species-checked": "guaranteed", "sELISA-species-dilution-info": "", "sELISA-species-notes": "<p></p>" } } }

style

left-column

Product details

ab285049 is a BSA and Azide Free antibody supplied in an unconjugated format and it is suitable for sandwich ELISAs to quantify Human TIMP4. The recommended pair for sandwich ELISA is:
Capture: ab285049, Human TIMP4 Capture Antibody (unconjugated)
Detector: ab285155, Human TIMP4 Detector Antibody (unconjugated)
The reference range value is 19.53-5000 pg/mL.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Sandwich ELISA
The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.

style

left-column

Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Tissue Inhibitor of Metalloproteinases 4 (TIMP4) a member of the TIMP family plays a mechanical role by inhibiting matrix metalloproteinases (MMPs). This inhibition helps regulate extracellular matrix (ECM) remodeling. TIMP4 weighs approximately 22 kDa and is expressed in various tissues including the heart brain and reproductive organs. It binds directly to MMPs forming stable complexes that prevent the degradation of ECM components.

Biological function summary

TIMP4 contributes to maintaining tissue homeostasis and integrity. By regulating ECM degradation it influences processes such as cell migration proliferation and angiogenesis. TIMP4 does not work alone; it associates with enzymes like MMP-2 and MMP-14 modulating their activities to keep ECM dynamics in check. This regulation is essential for normal physiological functions and also during tissue repair.

Pathways

TIMP4 participates in the balance between ECM synthesis and degradation. It takes part in the ECM receptor interaction and focal adhesion pathways. These pathways involve several proteins including integrins and collagens which interact with ECM components modulated by TIMP4 activity. TIMP4 helps coordinate cellular responses to environmental changes through these pathways thereby influencing cell behavior and tissue organization.

TIMP4 has associations with cardiovascular diseases and cancer. In cardiac contexts altered TIMP4 levels impact heart function and remodeling often linked to cardiovascular diseases. Its dysregulation can contribute to tumor progression in cancers by affecting ECM degradation and subsequent metastasis. TIMP4's interaction with proteins like MMP-9 links it to both tumor invasiveness and vascular remodeling in these disease states.

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

Complexes with metalloproteinases (such as collagenases) and irreversibly inactivates them by binding to their catalytic zinc cofactor. Known to act on MMP-1, MMP-2, MMP-3, MMP-7 and MMP-9.

See full target information Metalloproteinase inhibitor 4

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-TIMP4 antibody [EPR22830-311] - BSA and Azide free (Capture)

Sandwich ELISA - Anti-TIMP4 antibody [EPR22830-311] - BSA and Azide free (Capture) (AB285049)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Complementary Products

Assay Kits

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Product promise