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AB313425

Anti-TIMP4 antibody [RP23040074]

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Rabbit Recombinant Multiclonal TIMP4 antibody. Suitable for ICC/IF, WB, I-ELISA, Flow Cyt and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human Metalloproteinase inhibitor 4.

View Alternative Names

Metalloproteinase inhibitor 4, Tissue inhibitor of metalloproteinases 4, TIMP-4, TIMP4

5 Images
Immunocytochemistry/ Immunofluorescence - Anti-TIMP4 antibody [RP23040074] (AB313425)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-TIMP4 antibody [RP23040074] (AB313425)

Immunofluorescent analysis of 70% confluent log phase HeLa cells fixed with 4% paraformaldehyde for 15 minutes; permeabilized with 0.25% Triton X-100 for 10 minutes followed by blocking with 5% BSA for 1 hour at room temperature labeling TIMP4 with ab313425 at 1 µg-2 µg dilution, followed by Alexa Fluor 488 Goat anti-Rabbit IgG Secondary Antibody at 1/400 dilution for 30 minutes at room temperature (Panel a/ green). Nuclei (Panel b/ blue) were stained with DAPI. F-actin (Panel c/ red) was stained with Alexa Fluor 594 Phalloidin. Panel d is a merged image showing cytoplasmic localization of TIMP4. Panel e shows no primary antibody control. The images were captured at 20X magnification.

Flow Cytometry - Anti-TIMP4 antibody [RP23040074] (AB313425)
  • Flow Cyt

Supplier Data

Flow Cytometry - Anti-TIMP4 antibody [RP23040074] (AB313425)

Flow cytometry analysis of Hela cells fixed and permeabilized using FIX & PERM reagent labeling TIMP4 with ab313425. Detection was performed using an Alexa Fluor 488 Goat anti-Rabbit IgG (right peak) compared to an isotype control (left peak).

Immunocytochemistry/ Immunofluorescence - Anti-TIMP4 antibody [RP23040074] (AB313425)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-TIMP4 antibody [RP23040074] (AB313425)

Immunofluorescent analysis of U2OS cells fixed using 4% formaldehyde (reconstituted in 1X PBS) for 10 min at room temperature and permeabilized using 0.1 % Triton X-100 in PBS for 15 min at room temperature labeling TIMP4 with ab313425. followed by detection using an Alexa Fluor 488-conjugated Goat anti-Rabbit secondary antibody (green) (Image A). Nuclei were stained using DAPI (Image B) and actin stained with Alexa Fluor 594 phalloidin (red) (image C). Image D is a composite image showing cytoplasmic localization of TIMP4.

ELISA - Anti-TIMP4 antibody [RP23040074] (AB313425)
  • ELISA

Supplier Data

ELISA - Anti-TIMP4 antibody [RP23040074] (AB313425)

Indirect ELISA analysis of endogenous TIMP4 HeLa lysate coated onto the plate using ab313425 at various dilutions. A non-linear regression analysis was performed (4 PL) and LOD and LOQ for the antibody were determined.

Western blot - Anti-TIMP4 antibody [RP23040074] (AB313425)
  • WB

Supplier Data

Western blot - Anti-TIMP4 antibody [RP23040074] (AB313425)

All lanes:

Western blot - Anti-TIMP4 antibody [RP23040074] (ab313425) at 2 µg/mL

Lane 1:

HeLa cell lysate

Lane 2:

HEK293 cell lysate

Lane 3:

MDA-MB-231 cell lysate

Predicted band size: 26 kDa

Observed band size: 25 kDa

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Key facts

Host species

Rabbit

Clonality

Multiclonal

Clone number

RP23040074

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

I-ELISA, WB, Flow Cyt, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human Metalloproteinase inhibitor 4. The exact immunogen used to generate this antibody is proprietary information.

Q99727

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IELISA" : {"fullname" : "Indirect ELISA", "shortname":"I-ELISA"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1-2 µg/mL", "ICCIF-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1-3 µg/mL", "WB-species-notes": "<p></p>", "IELISA-species-checked": "testedAndGuaranteed", "IELISA-species-dilution-info": "", "IELISA-species-notes": "<p></p>", "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "2-4 µg for 10^6 Cells", "FlowCyt-species-notes": "<p></p>" } } }
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Product details

What are recombinant multiclonals?
Recombinant multiclonals are a mixture of recombinant antibodies co-expressed from a library of heavy and light chains. They offer several advantages including:

  • - The sensitivity of polyclonal antibodies by recognising multiple epitopes
  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

View our range of recombinant multiclonal antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
Preservative: 0.09% Sodium azide Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Tissue Inhibitor of Metalloproteinases 4 (TIMP4) a member of the TIMP family plays a mechanical role by inhibiting matrix metalloproteinases (MMPs). This inhibition helps regulate extracellular matrix (ECM) remodeling. TIMP4 weighs approximately 22 kDa and is expressed in various tissues including the heart brain and reproductive organs. It binds directly to MMPs forming stable complexes that prevent the degradation of ECM components.
Biological function summary

TIMP4 contributes to maintaining tissue homeostasis and integrity. By regulating ECM degradation it influences processes such as cell migration proliferation and angiogenesis. TIMP4 does not work alone; it associates with enzymes like MMP-2 and MMP-14 modulating their activities to keep ECM dynamics in check. This regulation is essential for normal physiological functions and also during tissue repair.

Pathways

TIMP4 participates in the balance between ECM synthesis and degradation. It takes part in the ECM receptor interaction and focal adhesion pathways. These pathways involve several proteins including integrins and collagens which interact with ECM components modulated by TIMP4 activity. TIMP4 helps coordinate cellular responses to environmental changes through these pathways thereby influencing cell behavior and tissue organization.

TIMP4 has associations with cardiovascular diseases and cancer. In cardiac contexts altered TIMP4 levels impact heart function and remodeling often linked to cardiovascular diseases. Its dysregulation can contribute to tumor progression in cancers by affecting ECM degradation and subsequent metastasis. TIMP4's interaction with proteins like MMP-9 links it to both tumor invasiveness and vascular remodeling in these disease states.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Complexes with metalloproteinases (such as collagenases) and irreversibly inactivates them by binding to their catalytic zinc cofactor. Known to act on MMP-1, MMP-2, MMP-3, MMP-7 and MMP-9.
See full target information Metalloproteinase inhibitor 4
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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