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Rabbit Multiclonal TIMP4 antibody. Suitable for ICC/IF, WB, I-ELISA, Flow Cyt and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human Metalloproteinase inhibitor 4.

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Images

Western blot - Anti-TIMP4 antibody [RP23040074] (AB313425), expandable thumbnail
  • ELISA - Anti-TIMP4 antibody [RP23040074] (AB313425), expandable thumbnail
  • Flow Cytometry - Anti-TIMP4 antibody [RP23040074] (AB313425), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-TIMP4 antibody [RP23040074] (AB313425), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-TIMP4 antibody [RP23040074] (AB313425), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

Preservative: 0.09% Sodium azide
Constituents: 99.91% PBS

Form
Liquid
Clonality
Multiclonal

Immunogen

  • Recombinant Fragment Protein within Human Metalloproteinase inhibitor 4. The exact immunogen used to generate this antibody is proprietary information. Database link Q99727

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
ICC/IFWBI-ELISAFlow Cyt
Human
Tested
Tested
Tested
Tested

Tested
Tested

Species
Human
Dilution info
1.00000-2.00000 µg/mL
Notes

-

Tested
Tested

Species
Human
Dilution info
1.00000-3.00000 µg/mL
Notes

-

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
2.00000-4.00000 µg for 106 Cells
Notes

-

Target data

Function

Complexes with metalloproteinases (such as collagenases) and irreversibly inactivates them by binding to their catalytic zinc cofactor. Known to act on MMP-1, MMP-2, MMP-3, MMP-7 and MMP-9.

Alternative names

Metalloproteinase inhibitor 4, Tissue inhibitor of metalloproteinases 4, TIMP-4, TIMP4

Recommended products

Rabbit Multiclonal TIMP4 antibody. Suitable for ICC/IF, WB, I-ELISA, Flow Cyt and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human Metalloproteinase inhibitor 4.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Multiclonal
Immunogen
  • Recombinant Fragment Protein within Human Metalloproteinase inhibitor 4. The exact immunogen used to generate this antibody is proprietary information. Database link Q99727
Clone number
RP23040074
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Recombinant multiclonals are a mixture of recombinant antibodies co-expressed from a library of heavy and light chains.

Recombinant multiclonal antibodies offer the sensitivity of polyclonal antibodies by recognising multiple epitopes, along with consistency of a recombinant antibody.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

Tissue Inhibitor of Metalloproteinases 4 (TIMP4) a member of the TIMP family plays a mechanical role by inhibiting matrix metalloproteinases (MMPs). This inhibition helps regulate extracellular matrix (ECM) remodeling. TIMP4 weighs approximately 22 kDa and is expressed in various tissues including the heart brain and reproductive organs. It binds directly to MMPs forming stable complexes that prevent the degradation of ECM components.

Biological function summary

TIMP4 contributes to maintaining tissue homeostasis and integrity. By regulating ECM degradation it influences processes such as cell migration proliferation and angiogenesis. TIMP4 does not work alone; it associates with enzymes like MMP-2 and MMP-14 modulating their activities to keep ECM dynamics in check. This regulation is essential for normal physiological functions and also during tissue repair.

Pathways

TIMP4 participates in the balance between ECM synthesis and degradation. It takes part in the ECM receptor interaction and focal adhesion pathways. These pathways involve several proteins including integrins and collagens which interact with ECM components modulated by TIMP4 activity. TIMP4 helps coordinate cellular responses to environmental changes through these pathways thereby influencing cell behavior and tissue organization.

Associated diseases and disorders

TIMP4 has associations with cardiovascular diseases and cancer. In cardiac contexts altered TIMP4 levels impact heart function and remodeling often linked to cardiovascular diseases. Its dysregulation can contribute to tumor progression in cancers by affecting ECM degradation and subsequent metastasis. TIMP4's interaction with proteins like MMP-9 links it to both tumor invasiveness and vascular remodeling in these disease states.

Product promise

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In the unlikely event of one of our products not working as expected, you are covered by our product promise.

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5 product images

  • Western blot - Anti-TIMP4 antibody [RP23040074] (ab313425), expandable thumbnail

    Western blot - Anti-TIMP4 antibody [RP23040074] (ab313425)

    All lanes: Western blot - Anti-TIMP4 antibody [RP23040074] (ab313425) at 2 µg/mL

    Lane 1: HeLa cell lysate

    Lane 2: HEK293 cell lysate

    Lane 3: MDA-MB-231 cell lysate

    Developed using the ECL technique.

    Predicted band size: 26 kDa

    Observed band size: 25 kDa

  • ELISA - Anti-TIMP4 antibody [RP23040074] (ab313425), expandable thumbnail

    ELISA - Anti-TIMP4 antibody [RP23040074] (ab313425)

    Indirect ELISA analysis of endogenous TIMP4 HeLa lysate coated onto the plate using ab313425 at various dilutions. A non-linear regression analysis was performed (4 PL) and LOD and LOQ for the antibody were determined.

  • Flow Cytometry - Anti-TIMP4 antibody [RP23040074] (ab313425), expandable thumbnail

    Flow Cytometry - Anti-TIMP4 antibody [RP23040074] (ab313425)

    Flow cytometry analysis of Hela cells fixed and permeabilized using FIX & PERM reagent labeling TIMP4 with ab313425. Detection was performed using an Alexa Fluor 488 Goat anti-Rabbit IgG (right peak) compared to an isotype control (left peak).

  • Immunocytochemistry/ Immunofluorescence - Anti-TIMP4 antibody [RP23040074] (ab313425), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-TIMP4 antibody [RP23040074] (ab313425)

    Immunofluorescent analysis of U2OS cells fixed using 4% formaldehyde (reconstituted in 1X PBS) for 10 min at room temperature and permeabilized using 0.1 % Triton X-100 in PBS for 15 min at room temperature labeling TIMP4 with ab313425. followed by detection using an Alexa Fluor 488-conjugated Goat anti-Rabbit secondary antibody (green) (Image A). Nuclei were stained using DAPI (Image B) and actin stained with Alexa Fluor 594 phalloidin (red) (image C). Image D is a composite image showing cytoplasmic localization of TIMP4.

  • Immunocytochemistry/ Immunofluorescence - Anti-TIMP4 antibody [RP23040074] (ab313425), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-TIMP4 antibody [RP23040074] (ab313425)

    Immunofluorescent analysis of 70% confluent log phase HeLa cells fixed with 4% paraformaldehyde for 15 minutes; permeabilized with 0.25% Triton X-100 for 10 minutes followed by blocking with 5% BSA for 1 hour at room temperature labeling TIMP4 with ab313425 at 1 ?g-2 ?g dilution, followed by Alexa Fluor 488 Goat anti-Rabbit IgG Secondary Antibody at 1/400 dilution for 30 minutes at room temperature (Panel a/ green). Nuclei (Panel b/ blue) were stained with DAPI. F-actin (Panel c/ red) was stained with Alexa Fluor 594 Phalloidin. Panel d is a merged image showing cytoplasmic localization of TIMP4. Panel e shows no primary antibody control. The images were captured at 20X magnification.

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Product protocols

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