AB181378

Anti-TIP30 antibody [EPR13185]

RabMAb
Recombinant
What is this?

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(1 Publication)

Rabbit Recombinant Monoclonal TIP30 antibody. Suitable for IP, WB, Flow Cyt (Intra) and reacts with Human samples. Cited in 1 publication.

View Alternative Names

CC3, TIP30, HTATIP2, Oxidoreductase HTATIP2, 30 kDa HIV-1 TAT-interacting protein, HIV-1 TAT-interactive protein 2

3 Images

Flow Cytometry (Intracellular) - Anti-TIP30 antibody [EPR13185] (AB181378)

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-TIP30 antibody [EPR13185] (AB181378)

Intracellular flow cytometric analysis of permeabilized HeLa cells (red) labeling TIP30 with ab181378 at 1/10 dilution (red), compared to a rabbit IgG negative control (green).

Supplier Data

Immunoprecipitation - Anti-TIP30 antibody [EPR13185] (AB181378)

Western blot analysis on immunoprecipitation pellet from A431 cell lysate labeling TIP30 with ab181378 at 1/10 dilution (Lane 1). Lane 2 is 1XPBS (negative control). Detection with HRP-conjugated anti-rabbit IgG, detecting the non-reduced form of rabbit IgG.

All lanes:

Immunoprecipitation - Anti-TIP30 antibody [EPR13185] (ab181378)

Predicted band size: 27 kDa

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Supplier Data

Western blot - Anti-TIP30 antibody [EPR13185] (AB181378)

All lanes:

Western blot - Anti-TIP30 antibody [EPR13185] (ab181378) at 1/10000 dilution

Lane 1:

Human fetal liver tissue lysate at 10 µg

Lane 2:

HeLa cell lysate at 10 µg

Lane 3:

A431 cell lysate at 10 µg

Lane 4:

A549 cell lysate at 10 µg

Lane 5:

SW480 cell lysate at 10 µg

Predicted band size: 27 kDa

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Carrier free

Anti-TIP30 antibody [EPR13185] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR13185

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, IP, Flow Cyt (Intra)

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/10 - 1/100", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10000 - 1/50000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/10 - 1/100", "FlowCytIntra-species-notes": "<a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody." } } }

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Product details

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purity

Tissue culture supernatant

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

TIP30 also known as HTATIP2 is a tumor suppressor protein that plays an important role in various cellular processes. It has a mass of approximately 30 kDa and is expressed in a range of human tissues including the liver lung and brain. TIP30 is an oxidoreductase and its activity involves the regulation of cellular responses to oxidative stress. It interacts with transcription factors and other proteins to modulate gene expression that is critical for cell growth and apoptosis.

Biological function summary

The TIP30 protein contributes to the regulation of gene transcription and the maintenance of cellular homeostasis. TIP30 is part of a complex that influences transcriptional activity by interacting with specific transcription factors. Its function suppresses oncogene activity and enhances the expression of genes that prevent tumor development. By controlling signaling pathways related to cell cycle and apoptosis TIP30 reinforces the protective mechanisms against cancer cell proliferation.

Pathways

TIP30 engages in important signaling pathways such as the PI3K/AKT pathway and the MAPK pathway where it acts to inhibit unwanted cell survival signals and tumorigenic processes. It interacts closely with proteins like AKT and ERK influencing their activity and ensuring appropriate responses to growth signals. TIP30 modulates cellular dynamics by regulating pathway fidelity which impacts cell survival proliferation and tumor suppression.

TIP30 levels are notably altered in certain cancer types such as hepatocellular carcinoma and breast cancer. These disorders correlate with its role in inhibiting tumor progression. Proteins such as c-MYC and ERBB2 often show varied expression in relation to TIP30 suggesting significant links in pathways leading to tumor growth. By investigating TIP30 involvement in these conditions researchers hope to identify potential therapeutic avenues that could restore its tumor suppressor function and curb cancer development.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Oxidoreductase required for tumor suppression. NADPH-bound form inhibits nuclear import by competing with nuclear import substrates for binding to a subset of nuclear transport receptors. May act as a redox sensor linked to transcription through regulation of nuclear import. Isoform 1 is a metastasis suppressor with proapoptotic as well as antiangiogenic properties. Isoform 2 has an antiapoptotic effect.

See full target information HTATIP2

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Molecular therapy. Nucleic acids 35:102221 PubMed38868363

2024

LNA-i-miR-221 activity in colorectal cancer: A reverse translational investigation.

Applications

Unspecified application

Species

Unspecified reactive species

Asad Ali,Katia Grillone,Serena Ascrizzi,Giulio Caridà,Lucia Fiorillo,Domenico Ciliberto,Nicoletta Staropoli,Pierosandro Tagliaferri,Pierfrancesco Tassone,Maria Teresa Di Martino

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-TIP30 antibody [EPR13185]

Flow Cytometry (Intracellular) - Anti-TIP30 antibody [EPR13185] (AB181378)

Immunoprecipitation - Anti-TIP30 antibody [EPR13185] (AB181378)

Western blot - Anti-TIP30 antibody [EPR13185] (AB181378)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purity

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

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