Anti-TIP49A antibody [2943C1a]

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TIP49A antibody [2943C1a] (AB51500)

IHC image of ab51500 staining in human normal testis formalin fixed paraffin embedded tissue section, performed on a Leica Bond^TM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab51500, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

• WB

AbReview28290****

Western blot - Anti-TIP49A antibody [2943C1a] (AB51500)

Excellent antibody. Detects a single species of the predicted mobility in a range of common rat cell lines.

All lanes:

Western blot - Anti-TIP49A antibody [2943C1a] (ab51500) at 1/100 dilution

Lane 1:

Whole cell lysate prepared from 9L cells at 20 µg

Lane 2:

Whole cell lysate prepared from PC-12 (Rat adrenal gland pheochromocytoma) cells at 20 µg

Lane 3:

Whole cell lysate prepared from RIE-1 cells at 20 µg

Secondary

All lanes:

HRP conjugated goat anti-mouse polyclonal at 1/5000 dilution

Predicted band size: 50 kDa

Observed band size: 57 kDa

true

Exposure time: 4min

Image courtesy of an anonymous Abreview.

• WB

AbReview19329****

Western blot - Anti-TIP49A antibody [2943C1a] (AB51500)

Blocking Step : 3% BSA for 1 hour at 21°C

All lanes:

Western blot - Anti-TIP49A antibody [2943C1a] (ab51500) at 1 µg/mL

Lanes 1 - 2:

HeLa cell lysate + control siRNA sequence at 20 µg

Lane 3:

HeLa cell lysate suppressed by siRNA (1) sequence to pontin at 20 µg

Lane 4:

HeLa cell lysate suppressed by siRNA (2) sequence to pontin at 20 µg

Lane 5:

Cell lysate of 293T cells at 20 µg

Lane 6:

Cell lysate of HeLa cells at 20 µg

Lane 7:

Cell lysate of U251 cells at 20 µg

Lane 8:

Cell lysate of Jurkat cells at 20 µg

Lane 9:

Cell lysate of U2OS cells at 20 µg

Secondary

All lanes:

An HRP-conjugated Goat anti-mouse IgG polyclonal at 1/5000 dilution

Predicted band size: 50 kDa

Observed band size: 57 kDa

true

Exposure time: 2min

This image is courtesy of an anonymous Abreview

• Flow Cyt

Unknown

Flow Cytometry - Anti-TIP49A antibody [2943C1a] (AB51500)

Flow cytometry using ab51500 at 0.5ug using HeLa cells. Secondary used was an AlexaFluor® 488 conjugated Goat Anti-mouse IgG (0.25 µg). White peak shows an isotype control, while the red peak shows ab51500.

• WB

Unknown

Western blot - Anti-TIP49A antibody [2943C1a] (AB51500)

All lanes:

Western blot - Anti-TIP49A antibody [2943C1a] (ab51500) at 1/100 dilution

All lanes:

HT-1080 whole cell lysate at 50 µg

Secondary

All lanes:

Anti Mouse IgG at 1/2500 dilution

Predicted band size: 50 kDa

Observed band size: 50 kDa

false