AB226001

Anti-TIP49A antibody

Name: Anti-TIP49A antibody (ab226001) | Abcam
Brand: Abcam Limited
SKU: AB226001
Price: 525 USD
Availability: InStock
Rating: 5 (1 reviews)

(1 Review)

(2 Publications)

Rabbit Polyclonal TIP49A antibody. Suitable for IP, WB and reacts with Human, Mouse samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human RUVBL1 aa 200-250.

View Alternative Names

INO80H, NMP238, TIP49, TIP49A, RUVBL1, RuvB-like 1, 49 kDa TATA box-binding protein-interacting protein, 54 kDa erythrocyte cytosolic protein, INO80 complex subunit H, Nuclear matrix protein 238, Pontin 52, TIP49a, TIP60-associated protein 54-alpha, 49 kDa TBP-interacting protein, ECP-54, NMP 238, TAP54-alpha

2 Images

Supplier Data

Immunoprecipitation - Anti-TIP49A antibody (AB226001)

TIP49A was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (prepared using NETN lysis buffer; 1 mg for IP, 20% of IP loaded) with ab226001 at 6 μg/mg lysate. Western blot was performed from the immunoprecipitate using ab226001 at 0.4 μg/ml.

Lane 1 : ab226001 IP in HEK-293T whole cell lysate.

Lane 2 : Control IgG IP in HEK-293T whole cell lysate.

Detection : Chemiluminescence with exposure time of 10 seconds.

All lanes:

Immunoprecipitation - Anti-TIP49A antibody (ab226001)

Predicted band size: 50 kDa

false

Supplier Data

Western blot - Anti-TIP49A antibody (AB226001)

Lysates prepared using NETN lysis buffer.

All lanes:

Western blot - Anti-TIP49A antibody (ab226001) at 0.1 µg/mL

Lane 1:

HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg

Lane 2:

HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg

Lane 3:

Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate at 50 µg

Lane 4:

TCMK-1 (mouse kidney epithelial cell line) whole cell lysate at 50 µg

Lane 5:

NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate at 50 µg

Predicted band size: 50 kDa

true

Exposure time: 30s

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

WB, IP

applications

Immunogen

Synthetic Peptide within Human RUVBL1 aa 200-250. The exact immunogen used to generate this antibody is proprietary information.

Q9Y265

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-10 µg/mg of lysate", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "" }, "Cow": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Dog": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Drosophila melanogaster": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Gorilla": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Horse": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Zebrafish": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

ab226001 was affinity purified using an epitope specific to TIP49A immobilized on solid support.

Storage buffer

pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: Tris citrate/phosphate

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

TIP49A also known as RUVBL1 or Pontin is a 54 kDa protein that plays a mechanical role in various cellular processes. TIP49A functions as an ATPase with a part in the regulation of the chromatin structure and remodeling. It is expressed in the nucleus and cytoplasm of many cell types indicating its broad involvement in cellular activities. Being highly evolutionarily conserved TIP49A shows a strong degree of structural conservation which hints at its essential roles maintained across species.

Biological function summary

TIP49A contributes to the assembly and function of multiprotein complexes. It works closely as part of the INO80 and TIP60 chromatin remodeling complexes which are important for transcription regulation and DNA repair. Through these associations TIP49A is key in controlling access to DNA impacting gene expression and stability of chromosomes. Its role in mediating interactions between different protein partners underlines its importance in maintaining cellular homeostasis and response to DNA damage.

Pathways

TIP49A participates in essential cellular processes like transcriptional regulation and DNA repair. In the context of transcription regulation TIP49A operates alongside MYC a well-known oncogene to influence gene expression linked to cell proliferation. It also plays a significant role in the homologous recombination repair pathway interacting with proteins like RAD51 to ensure proper repair of DNA double-strand breaks. These pathways demonstrate the integral function of TIP49A in preserving genomic integrity and normal cell cycle progression.

TIP49A has links to cancer progression and neurodegenerative diseases. Overexpression of TIP49A is observed in various cancers including breast and colorectal cancer where it correlates with tumor growth and poor prognosis. It interacts with c-Myc a protein with significant oncogenic potential to drive aberrant cell proliferation. In neurodegenerative conditions such as Alzheimer's disease TIP49A's connection to chromatin remodeling dysregulation is of interest although research is still ongoing to fully elucidate this relationship.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
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Target data

Possesses single-stranded DNA-stimulated ATPase and ATP-dependent DNA helicase (3' to 5') activity; hexamerization is thought to be critical for ATP hydrolysis and adjacent subunits in the ring-like structure contribute to the ATPase activity (PubMed : 17157868, PubMed : 33205750). Component of the NuA4 histone acetyltransferase complex which is involved in transcriptional activation of select genes principally by acetylation of nucleosomal histones H4 and H2A (PubMed : 14966270). This modification may both alter nucleosome-DNA interactions and promote interaction of the modified histones with other proteins which positively regulate transcription (PubMed : 14966270). This complex may be required for the activation of transcriptional programs associated with oncogene and proto-oncogene mediated growth induction, tumor suppressor mediated growth arrest and replicative senescence, apoptosis, and DNA repair (PubMed : 14966270). The NuA4 complex ATPase and helicase activities seem to be, at least in part, contributed by the association of RUVBL1 and RUVBL2 with EP400. NuA4 may also play a direct role in DNA repair when recruited to sites of DNA damage (PubMed : 14966270). Component of a SWR1-like complex that specifically mediates the removal of histone H2A.Z/H2AZ1 from the nucleosome (PubMed : 24463511). Proposed core component of the chromatin remodeling INO80 complex which exhibits DNA- and nucleosome-activated ATPase activity and catalyzes ATP-dependent nucleosome sliding (PubMed : 16230350, PubMed : 21303910). Plays an essential role in oncogenic transformation by MYC and also modulates transcriptional activation by the LEF1/TCF1-CTNNB1 complex (PubMed : 10882073, PubMed : 16014379). Essential for cell proliferation (PubMed : 14506706). May be able to bind plasminogen at cell surface and enhance plasminogen activation (PubMed : 11027681).

See full target information RUVBL1

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Applied and environmental microbiology 89:e0066623 PubMed37668382

2023

Development of heat-shock resistance in modeled by experimental evolution.

Applications

Unspecified application

Species

Unspecified reactive species

Jeffrey Liang,Gillian Cameron,Sébastien P Faucher

EMBO reports 23:e54128 PubMed35876654

2022

LINC00839 promotes colorectal cancer progression by recruiting RUVBL1/Tip60 complexes to activate NRF1.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaoting Liu,Jianxiong Chen,Sijing Zhang,Xunhua Liu,Xiaoli Long,Jiawen Lan,Miao Zhou,Lin Zheng,Jun Zhou

View all publications

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