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Rabbit Recombinant Monoclonal TIPE2 antibody. Carrier free. Suitable for sELISA and reacts with Human, Mouse, Rat samples.

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Images

Sandwich ELISA - Anti-TIPE2 antibody [EPR27964-69] - BSA and Azide free (Detector) (AB317349), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Constituents: 100% PBS

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
sELISA
Human
Tested
Mouse
Tested
Rat
Tested

Tested
Tested

Species
Human, Mouse, Rat
Dilution info
-
Notes

-

Target data

Function

Acts as a negative regulator of innate and adaptive immunity by maintaining immune homeostasis (PubMed:27043859). Plays a regulatory role in the Toll-like signaling pathway by determining the strength of LPS-induced signaling and gene expression (PubMed:32188758). Inhibits TCR-mediated T-cell activation and negatively regulate T-cell function to prevent hyperresponsiveness (By similarity). Inhibits also autolysosome formation via negatively modulating MTOR activation by interacting with RAC1 and promoting the disassociation of the RAC1-MTOR complex (PubMed:32460619). Plays an essential role in NK-cell biology by acting as a checkpoint and displaying an expression pattern correlating with NK-cell maturation process and by negatively regulating NK-cell maturation and antitumor immunity (By similarity). Mechanistically, suppresses IL-15-triggered mTOR activity in NK-cells (By similarity).

Alternative names

Tumor necrosis factor alpha-induced protein 8-like protein 2, TIPE2, TNF alpha-induced protein 8-like protein 2, TNFAIP8-like protein 2, Inflammation factor protein 20, TNFAIP8L2

Recommended products

Rabbit Recombinant Monoclonal TIPE2 antibody. Carrier free. Suitable for sELISA and reacts with Human, Mouse, Rat samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free
Yes
Clone number
EPR27964-69
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C

Notes

ab317349 is a BSA and Azide free antibody supplied in an unconjugated
format and it is suitable for sandwich ELISAs to quantify TIPE2.
The recommended pair for sandwich ELISA is:

Capture: Anti-TIPE2 antibody [EPR27964-93] - BSA and Azide free (Capture) ab317348, TIPE2 Capture Antibody (unconjugated)

Detector: ab317349, TIPE2 Detector Antibody (unconjugated)

The reference range value is 7.8-2000 pg/mL.

The recommended antibody orientation is based on internal optimization
for ELISAbased assays. Antibody orientation is assay dependent and
needs to be optimized for each assay type. Please note that the
range provided for this antibody is only an estimation based on the
performance of the product using the recommended antibody pair.
Performance of the antibody pair will depend on the specific
characteristics of your assay. We guarantee the product works in
sandwich ELISA, but we do not guarantee the sensitivity or dynamic
range of the antibody in your assay.

Our carrier-free formats are supplied in a buffer free of BSA,
sodium azide and glycerol for higher conjugation efficiency.
The antibodies are provided at an approximate concentration of
1 mg/ml as measured by the protein A280 method. Use our conjugation
kits for antibody conjugates that are ready-to-use in as
little as 20 minutes with <1 minute hands-on-time and 100% antibody
recovery: available for fluorescent dyes, HRP, biotin and gold.

The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

TIPE2 also known as TNFAIP8L2 is a member of the TNFAIP8 family of proteins with a molecular mass of approximately 23 kDa. Mechanically TIPE2 acts as a regulator of cell death and survival processes playing a role in modulating immune responses. It is expressed in a variety of tissues but particularly abundant in immune-related cells such as macrophages and lymphocytes. The protein influences signaling pathways by interacting with other molecules in the cell affecting downstream responses.

Biological function summary

TIPE2 functions as a negative regulator of immune signaling pathways providing a check against excessive immune activation. It is a cytosolic protein that does not form part of a larger protein complex but can interact with membrane-associated signaling proteins. TIPE2 inhibits both NF-kB and MAPK signaling pathways which are important in mediating immune responses and inflammation. This regulatory function is essential for maintaining immune homeostasis and preventing inflammatory disorders.

Pathways

Research has highlighted that TIPE2 is integrated within immune signaling cascades particularly impacting the NF-kB and MAPK pathways. These pathways play pivotal roles in inflammation and immune system activation. TIPE2 acts to inhibit the activation of proteins like IKK and JNK which are key mediators within these pathways. By suppressing these pathways TIPE2 helps to balance immune responses and prevent overactivation that can lead to disease states.

Associated diseases and disorders

TIPE2 has been associated with autoimmune diseases and cancer. Insufficient TIPE2 expression or function can lead to autoimmune disorders like rheumatoid arthritis where unchecked immune signaling causes tissue damage. Conversely overexpression might contribute to cancer progression by dampening anti-tumor immune responses. TIPE2’s interaction with TNFAIP8 family proteins has shown implications in tumor progression highlighting its dual role in modulating immune responses and potential for therapeutic targeting.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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