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AB229329

Anti-TIPIN antibody

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(3 Publications)

Rabbit Polyclonal TIPIN antibody. Suitable for WB and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Human TIMELESS-interacting protein.

View Alternative Names

TIMELESS-interacting protein, TIPIN

2 Images
Western blot - Anti-TIPIN antibody (AB229329)
  • WB

Supplier Data

Western blot - Anti-TIPIN antibody (AB229329)

10 % SDS-PAGE gel.

All lanes:

Western blot - Anti-TIPIN antibody (ab229329) at 1/1000 dilution

Lane 1:

HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 30 µg

Lane 2:

A431 (human epidermoid carcinoma cell line) whole cell lysate at 30 µg

Lane 3:

HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 30 µg

Lane 4:

HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate at 30 µg

Predicted band size: 35 kDa

false

Western blot - Anti-TIPIN antibody (AB229329)
  • WB

Supplier Data

Western blot - Anti-TIPIN antibody (AB229329)

12 % SDS-PAGE gel.

All lanes:

Western blot - Anti-TIPIN antibody (ab229329) at 1/5000 dilution

Lane 1:

HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 30 µg

Lane 2:

TIPIN-transfected HEK-293T whole cell lysate at 30 µg

Predicted band size: 35 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB

applications

Immunogen

Recombinant Fragment Protein within Human TIMELESS-interacting protein. The exact immunogen used to generate this antibody is proprietary information.

Q9BVW5

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 Preservative: 0.025% Proclin 300 Constituents: PBS, 20% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The TIPIN protein also known as Timekeeper Homolog is a 29 kDa protein involved in the regulation of DNA replication and genome stability. It interacts with other proteins to form a complex that plays a role in stabilizing the replication machinery. TIPIN expresses in various tissues including those with high proliferation rates. Researchers study its mechanical role because of its involvement in maintaining the proper functioning of the replication fork a critical part of DNA synthesis.
Biological function summary

The interaction of TIPIN in the replication fork protection complex is important for preventing genomic instability. TIPIN associates with the Timeless protein to form an important complex that maintains the integrity of DNA replication under stress conditions. This complex stabilizes replication forks and prevents DNA breakage allowing smooth progression of DNA synthesis. Without its function cells might encounter failed replication processes and increased mutations.

Pathways

TIPIN integrates into the DNA damage response pathway which maintains genome integrity by activating repair processes. It interacts with the ATR-Chk1 signaling pathway an important mediator of replication stress response. This pathway triggers cell cycle arrest to give cells time to repair damaged DNA. TIPIN collaborates with Timeless and other proteins like MCM helicase preventing replication collapse and maintaining genomic stall points.

TIPIN's role in maintaining genomic stability connects it with cancer development where DNA replication errors can promote tumorigenesis. Changes or mutations affecting TIPIN expression can contribute to breast cancer. Additionally TIPIN is linked to ATR mutations in rare genetic disorders like Seckel syndrome where defective DNA repair causes developmental problems. The relationship between TIPIN and these proteins highlights its possible impact on such diseases serving as a target for therapeutic interventions.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Plays an important role in the control of DNA replication and the maintenance of replication fork stability (PubMed : 17102137, PubMed : 23359676, PubMed : 35585232). Important for cell survival after DNA damage or replication stress (PubMed : 17116885). May be specifically required for the ATR-CHEK1 pathway in the replication checkpoint induced by hydroxyurea or ultraviolet light (PubMed : 17296725). Forms a complex with TIMELESS and this complex regulates DNA replication processes under both normal and stress conditions, stabilizes replication forks and influences both CHEK1 phosphorylation and the intra-S phase checkpoint in response to genotoxic stress (PubMed : 17102137, PubMed : 17116885, PubMed : 17296725, PubMed : 23359676, PubMed : 35585232).
See full target information TIMELESS-interacting protein
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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Nutrition & metabolism 22:109 PubMed41029463

2025

mTOR-autophagy axis regulation by intermittent fasting promotes skeletal muscle growth and differentiation.

Applications

Unspecified application

Species

Unspecified reactive species

Chen Xinyan,Wu Yajie,He Shangfan,Yang Yuefei,Li Junwei,Zhu Jiaqiao,Ju Huiming

Molecular cancer therapeutics 23:911-923 PubMed38466804

2024

The Novel ATR Inhibitor M1774 Induces Replication Protein Overexpression and Broad Synergy with DNA-targeted Anticancer Drugs.

Applications

Unspecified application

Species

Unspecified reactive species

Ukhyun Jo,Yasuhiro Arakawa,Astrid Zimmermann,Daiki Taniyama,Makito Mizunuma,Lisa M Jenkins,Tapan Maity,Suresh Kumar,Frank T Zenke,Naoko Takebe,Yves Pommier

eLife 10: PubMed34608864

2021

DDK/Hsk1 phosphorylates and targets fission yeast histone deacetylase Hst4 for degradation to stabilize stalled DNA replication forks.

Applications

Unspecified application

Species

Unspecified reactive species

Shalini Aricthota,Devyani Haldar
View all publications
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