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AB236451

Anti-TIPIN antibody

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(1 Publication)

Rabbit Polyclonal TIPIN antibody. N-terminal. Suitable for IP, WB, ICC/IF and reacts with Human, Mouse samples. Cited in 1 publication. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human TIMELESS-interacting protein.

View Alternative Names

TIMELESS-interacting protein, TIPIN

3 Images
Immunocytochemistry/ Immunofluorescence - Anti-TIPIN antibody (AB236451)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-TIPIN antibody (AB236451)

2% paraformaldehyde-fixed U-2 OS (Human bone osteosarcoma epithelial cell line) cells labeling TIPIN with ab236451 (green) at 1/600 dilution in ICC/IF. Cells were treated with siRNA against TIPIN (right panel) or control (left panel). Counter stained with DAPI (blue). Incubated with primary antibody overnight at +4°C.

Immunoprecipitation - Anti-TIPIN antibody (AB236451)
  • IP

Supplier Data

Immunoprecipitation - Anti-TIPIN antibody (AB236451)

TIPIN was immunoprecipitated from U-2 OS (Human bone osteosarcoma epithelial cell line) whole cell lysate using 3 μl of ab236451.

Lane 1 : U-2 OS whole cell lysate ~400 μg (Input).

Lane 2 : Control IgG in U-2 OS whole cell lysate.

Lane 3 : ab236451 in U-2 OS whole cell lysate.

For western blotting ab236451 was used at a 1/2000 dilution.

All lanes:

Immunoprecipitation - Anti-TIPIN antibody (ab236451)

Predicted band size: 35 kDa

false

Western blot - Anti-TIPIN antibody (AB236451)
  • WB

Supplier Data

Western blot - Anti-TIPIN antibody (AB236451)

Blocking : 5% TBS-T milk.

Incubated with primary antibody overnight.

All lanes:

Western blot - Anti-TIPIN antibody (ab236451) at 1/2000 dilution

Lane 1:

U-2 OS (Human bone osteosarcoma epithelial cell line) treated with control siRNA cell lysate at 40 µg

Lane 2:

U-2 OS (Human bone osteosarcoma epithelial cell line) treated with TIPIN siRNA cell lysate at 40 µg

Lane 3:

NIH/3T3 (Mouse embryo fibroblast cell line) cell lysate at 40 µg

Predicted band size: 35 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Human

Applications

IP, WB, ICC/IF

applications

Immunogen

Recombinant Full Length Protein corresponding to Human TIMELESS-interacting protein.

Q9BVW5

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/2000", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/600", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purity
Whole antiserum
Storage buffer
Preservative: 0.1% Sodium azide
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The TIPIN protein also known as Timekeeper Homolog is a 29 kDa protein involved in the regulation of DNA replication and genome stability. It interacts with other proteins to form a complex that plays a role in stabilizing the replication machinery. TIPIN expresses in various tissues including those with high proliferation rates. Researchers study its mechanical role because of its involvement in maintaining the proper functioning of the replication fork a critical part of DNA synthesis.
Biological function summary

The interaction of TIPIN in the replication fork protection complex is important for preventing genomic instability. TIPIN associates with the Timeless protein to form an important complex that maintains the integrity of DNA replication under stress conditions. This complex stabilizes replication forks and prevents DNA breakage allowing smooth progression of DNA synthesis. Without its function cells might encounter failed replication processes and increased mutations.

Pathways

TIPIN integrates into the DNA damage response pathway which maintains genome integrity by activating repair processes. It interacts with the ATR-Chk1 signaling pathway an important mediator of replication stress response. This pathway triggers cell cycle arrest to give cells time to repair damaged DNA. TIPIN collaborates with Timeless and other proteins like MCM helicase preventing replication collapse and maintaining genomic stall points.

TIPIN's role in maintaining genomic stability connects it with cancer development where DNA replication errors can promote tumorigenesis. Changes or mutations affecting TIPIN expression can contribute to breast cancer. Additionally TIPIN is linked to ATR mutations in rare genetic disorders like Seckel syndrome where defective DNA repair causes developmental problems. The relationship between TIPIN and these proteins highlights its possible impact on such diseases serving as a target for therapeutic interventions.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Plays an important role in the control of DNA replication and the maintenance of replication fork stability (PubMed : 17102137, PubMed : 23359676, PubMed : 35585232). Important for cell survival after DNA damage or replication stress (PubMed : 17116885). May be specifically required for the ATR-CHEK1 pathway in the replication checkpoint induced by hydroxyurea or ultraviolet light (PubMed : 17296725). Forms a complex with TIMELESS and this complex regulates DNA replication processes under both normal and stress conditions, stabilizes replication forks and influences both CHEK1 phosphorylation and the intra-S phase checkpoint in response to genotoxic stress (PubMed : 17102137, PubMed : 17116885, PubMed : 17296725, PubMed : 23359676, PubMed : 35585232).
See full target information TIMELESS-interacting protein
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

British journal of pharmacology 182:3594-3611 PubMed40194519

2025

Inhibition of DNA polymerase eta-mediated translesion DNA synthesis with small molecule sensitises ovarian cancer stem-like cells to chemotherapy.

Applications

Unspecified application

Species

Unspecified reactive species

Subhankar Bose,Priyanka Saha,Md Tanjim Alam,Bilash Chatterjee,Mrinmoy Sarkar,Amit Kumar Dixit,Deepak Kumar,Rakesh Kumar Pathak,Prem Prakash Tripathi,Amit Kumar Srivastava
View all publications
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