AB70795

Anti-TIPRL antibody

Name: Anti-TIPRL antibody (ab70795) | Abcam
Brand: Abcam Limited
SKU: AB70795
Price: 545 USD
Availability: InStock

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(6 Publications)

Rabbit Polyclonal TIPRL antibody. Suitable for IP, WB, IHC-P and reacts with Human, Mouse samples. Cited in 6 publications. Immunogen corresponding to Synthetic Peptide within Human TIPRL.

View Alternative Names

TIP41-like protein, Putative MAPK-activating protein PM10, Type 2A-interacting protein, TIP, TIPRL

3 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TIPRL antibody (AB70795)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TIPRL antibody (AB70795)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human ovarian carcinoma tissue labelling TIPRL with ab70795 at 1/200 (1µg/ml). Detection : DAB.

Unknown

Immunoprecipitation - Anti-TIPRL antibody (AB70795)

Detection of TIPRL by Western Blot of Immunoprecipitate.
ab70795 at 1µg/ml staining TIPRL in HeLa whole cell lysate immunoprecipitated using ab70795 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Detection : Chemiluminescence with exposure time of 3 seconds.

All lanes:

Immunoprecipitation - Anti-TIPRL antibody (ab70795)

Predicted band size: 31 kDa

false

Unknown

Western blot - Anti-TIPRL antibody (AB70795)

All lanes:

Western blot - Anti-TIPRL antibody (ab70795) at 0.04 µg/mL

Lane 1:

HeLa whole cell lysate at 50 µg/mL

Lane 2:

HeLa whole cell lysate at 15 µg/mL

Lane 3:

HeLa whole cell lysate at 5 µg/mL

Lane 4:

293T whole cell lysate at 50 µg/mL

Lane 5:

NIH3T3 whole cell lysate at 50 µg/mL

Predicted band size: 31 kDa

Observed band size: 32 kDa

true

Exposure time: 3s

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

IHC-P, IP, WB

applications

Immunogen

Synthetic Peptide within Human TIPRL. The exact immunogen used to generate this antibody is proprietary information.

O75663

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-5 µg/mg of lysate", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/500", "IHCP-species-notes": "" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Chimpanzee": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Orangutan": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rhesus monkey": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

ab70795 was affinity purified using an epitope specific to TIPRL immobilized on solid support.

Storage buffer

pH: 6.8 - 7.4 Preservative: 0.09% Sodium azide Constituents: Tris buffered saline, 0.1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

TIPRL also known as TIP41-like protein weighs approximately 39 kDa. It functions by interacting with the alpha subunit of protein phosphatase 2A (PP2A) which acts as a negative regulator within various cellular processes. TIPRL is expressed in a variety of tissues with notable presence in the liver kidney and brain. Its modulation role occurs through binding to other proteins and influencing catalytic activities important for maintaining balance in cellular signaling.

Biological function summary

TIPRL serves a regulatory role by inhibiting phosphatase activities within the cell contributing significantly to protein dephosphorylation processes. This protein is a part of the PP2A holoenzyme complex where it serves to fine-tune its activity affecting signaling pathways that rely on phosphorylation status. Through associating with PP2A and related phosphatases TIPRL ensures proper cellular phosphoprotein turnover an important factor for healthy cellular function and response to external stimuli.

Pathways

Interaction between TIPRL and other proteins influences the mTOR signaling pathway and MAPK signaling pathways. Through these pathways TIPRL affects cellular growth proliferation and survival. TIPRL influences mTOR pathway by modulating the activity of PP2A which can affect downstream proteins like AKT and S6K1. The MAPK pathway is impacted similarly where TIPRL's interaction with PP2A alters phosphorylation state of proteins such as ERK creating implications for cell cycle progression and stress response.

TIPRL is linked to neurological disorders and certain cancers. TIPRL's regulation of pathways like mTOR and MAPK associates it with the development of glioblastomas where dysregulated signaling contributes to tumorigenesis. Additionally in neurodegenerative conditions altered TIPRL function affects phosphatase activity impacting proteins like Tau which play a role in diseases like Alzheimer's. Understanding TIPRL's modulation could provide insights into therapeutic targets for these conditions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

May be a allosteric regulator of serine/threonine-protein phosphatase 2A (PP2A). Isoform 1 inhibits catalytic activity of the PP2A(D) core complex in vitro. The PP2A(C) : TIPRL complex does not show phosphatase activity. Acts as a negative regulator of serine/threonine-protein phosphatase 4 probably by inhibiting the formation of the active PPP4C : PPP4R2 complex; the function is proposed to implicate it in DNA damage response by promoting H2AX phosphorylated on Ser-140 (gamma-H2AX). May play a role in the regulation of ATM/ATR signaling pathway controlling DNA replication and repair.

See full target information TIPRL

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Publications (6)

Recent publications for all applications. Explore the full list and refine your search

Brain : a journal of neurology 146:2612-2626 PubMed36385662

2022

Sex bias in social deficits, neural circuits and nutrient demand in Cttnbp2 autism models.

Applications

Unspecified application

Species

Unspecified reactive species

Tzu-Li Yen,Tzyy-Nan Huang,Ming-Hui Lin,Tsan-Ting Hsu,Ming-Hsuan Lu,Pu-Yun Shih,Jacob Ellegood,Jason Lerch,Yi-Ping Hsueh

Frontiers in oncology 10:1062 PubMed32719745

2020

TIPRL, a Novel Tumor Suppressor, Suppresses Cell Migration, and Invasion Through Regulating AMPK/mTOR Signaling Pathway in Gastric Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Meng Luan,Shan-Shan Shi,Duan-Bo Shi,Hai-Ting Liu,Ran-Ran Ma,Xiao-Qun Xu,Yu-Jing Sun,Peng Gao

Oncology letters 20:43-52 PubMed32565932

2020

YRDC is upregulated in non-small cell lung cancer and promotes cell proliferation by decreasing cell apoptosis.

Applications

Unspecified application

Species

Unspecified reactive species

Haibo Shen,Enkuo Zheng,Zhenhua Yang,Minglei Yang,Xiang Xu,Yinjie Zhou,Junjun Ni,Rui Li,Guofang Zhao

Experimental and therapeutic medicine 19:1925-1932 PubMed32104250

2020

Knockdown of TOR signaling pathway regulator suppresses cell migration and invasion in non-small cell lung cancer via the regulation of epithelial-to-mesenchymal transition.

Applications

Unspecified application

Species

Unspecified reactive species

Xiang Xu,Huangkai Zhu,Minglei Yang,Enkuo Zheng,Yinjie Zhou,Junjun Ni,Rui Li,Zhenhua Yang,Ti He,Guofang Zhao

Pathology, research and practice 216:152836 PubMed32014308

2020

NUP37 silencing induces inhibition of cell proliferation, G1 phase cell cycle arrest and apoptosis in non-small cell lung cancer cells.

Applications

Unspecified application

Species

Unspecified reactive species

Lianglong Huang,Tao Wang,Fumu Wang,Xiaoyi Hu,Guoxiang Zhan,Xiaohui Jin,Licheng Zhang,Yuping Li

PLoS pathogens 11:e1005288 PubMed26599541

2015

The Transcription and Translation Landscapes during Human Cytomegalovirus Infection Reveal Novel Host-Pathogen Interactions.

Applications

Species

Unspecified reactive species

Osnat Tirosh,Yifat Cohen,Alina Shitrit,Odem Shani,Vu Thuy Khanh Le-Trilling,Mirko Trilling,Gilgi Friedlander,Marvin Tanenbaum,Noam Stern-Ginossar

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-TIPRL antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TIPRL antibody (AB70795)

Immunoprecipitation - Anti-TIPRL antibody (AB70795)

Western blot - Anti-TIPRL antibody (AB70795)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (6)

Product promise