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AB13684

Anti-TIRAP antibody [20D1055]

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(2 Publications)

Mouse Monoclonal TIRAP antibody. Suitable for WB and reacts with Human samples. Cited in 2 publications.

View Alternative Names

Adapter protein wyatt, FLJ42305, MyD88 adapter like protein, TIR domain containing adapter protein, Toll interleukin 1 receptor (TIR) domain containing adaptor protein, Toll interleukin 1 receptor domain containing adapter protein, Toll like receptor adaptor protein, Wyatt

1 Images
Western blot - Anti-TIRAP antibody [20D1055] (AB13684)
  • WB

Unknown

Western blot - Anti-TIRAP antibody [20D1055] (AB13684)

Western blot analysis of TIRAP using ab13684 at 1 : 100 on 20 μgs of HUVEC cell lysate. Western blot analysis of TIRAP using ab13684 at 1 : 100 on 20 µgs of HUVEC cell lysate.

All lanes:

Western blot - Anti-TIRAP antibody [20D1055] (ab13684)

Predicted band size: 24 kDa

false

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

20D1055

Isotype

IgM

Carrier free

No

Reacts with

Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purity
Tissue culture supernatant
Storage buffer
Preservative: 0.05% Sodium azide
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

TIRAP also known as Mal is an adapter protein integral to the Toll-like receptor (TLR) signaling pathway. It has a molecular mass of approximately 24 kDa. TIRAP resides mostly in the cytoplasm and gets recruited to the plasma membrane where it assists in signal transduction. It plays a significant role in bridging TLRs such as TLR2 and TLR4 with downstream signaling elements like MyD88. This protein mediates the recruitment of other signaling proteins after TLR activation therefore facilitating the innate immune response.
Biological function summary

TIRAP is part of the innate immune system and contributes to the immune response against pathogens. It forms part of a signaling complex that leads to the activation of NF-kB and MAP kinase pathways resulting in the production of inflammatory cytokines. The protein works by directly interacting with phosphatidylinositol 45-bisphosphate at the plasma membrane through its phosphoinositide-binding motif. This interaction ensures that TIRAP is correctly positioned to relay signals for immune activation.

Pathways

TIRAP functions in the MyD88-dependent signaling pathway which is significant for initiating inflammatory responses. TIRAP and MyD88 work in tandem to propagate signals from activated TLRs. This relationship not only aids in inflammation but also in the differentiation of immune cells. TIRAP is also related to other proteins like IRAK1 in these pathways which further transduces signals leading to downstream activation of transcription factors relevant for immune responses.

TIRAP plays a part in conditions like systemic lupus erythematosus and septic shock. Mutations in the TIRAP gene can alter the signaling cascade leading to aberrant immune responses associated with these diseases. In this context TIRAP interacts with TLR4 and any dysfunction in the interaction may contribute to the excessive inflammation seen in septic shock. Understanding TIRAP's role in these diseases provides insight into targeted therapeutic strategies.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

See full target information TIRAP
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Journal of cellular physiology : PubMed30693511

2019

Overexpression of oncostatin M receptor regulates local immune response in glioblastoma.

Applications

Unspecified application

Species

Unspecified reactive species

Qing Guo,Ge-Fei Guan,Jing-Yuan Cao,Cun-Yi Zou,Chen Zhu,Wen Cheng,Xiao-Yan Xu,Zhi-Guo Lin,Peng Cheng,An-Hua Wu

American journal of respiratory cell and molecular 32:531-9 PubMed15778492

2005

Induction of CXCL5 during inflammation in the rodent lung involves activation of alveolar epithelium.

Applications

WB

Species

Mouse

Samithamby Jeyaseelan,Rizwan Manzer,Scott K Young,Masahiro Yamamoto,Shizuo Akira,Robert J Mason,G Scott Worthen
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Product promise

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