AB17218

Anti-TIRAP antibody

Name: Anti-TIRAP antibody (ab17218) | Abcam
Brand: Abcam Limited
SKU: AB17218
Rating: 5 (2 reviews)

(2 Reviews)

(16 Publications)

Rabbit Polyclonal TIRAP antibody. Suitable for WB, ICC/IF, IHC-P and reacts with Human, Mouse, Rat samples. Cited in 16 publications. Immunogen corresponding to Synthetic Peptide within Human TIRAP.

View Alternative Names

Adapter protein wyatt, FLJ42305, MyD88 adapter like protein, TIR domain containing adapter protein, Toll interleukin 1 receptor (TIR) domain containing adaptor protein, Toll interleukin 1 receptor domain containing adapter protein, Toll like receptor adaptor protein, Wyatt

2 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TIRAP antibody (AB17218)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TIRAP antibody (AB17218)

ab17218 at 2μg/ml staining TIRAP in human heart cells by IHC

Unknown

Western blot - Anti-TIRAP antibody (AB17218)

Western Blot of human heart lysate labeling TIRAP with Anti-TIRAP antibody (ab17218) at (1) 1 and (2) 2μg/ml.

All lanes:

Western blot - Anti-TIRAP antibody (ab17218)

Predicted band size: 24 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human, Rat, Mouse

Applications

WB, ICC/IF, IHC-P

applications

Immunogen

Synthetic Peptide within Human TIRAP. The exact immunogen used to generate this antibody is proprietary information.

P58753

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.5-2 µg/mL", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "10 µg/mL", "ICCIF-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "2 µg/mL", "IHCP-species-notes": "" }, "Mouse": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "0.5-2 µg/mL", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "10 µg/mL", "ICCIF-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "2 µg/mL", "IHCP-species-notes": "" }, "Rat": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "0.5-2 µg/mL", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "10 µg/mL", "ICCIF-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "2 µg/mL", "IHCP-species-notes": "" }, "Rhesus monkey": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

ab17218 is purified by ion exchange chromatography.TIRAP Antibody is affinity chromatography purified via peptide column.

Storage buffer

pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Avoid freeze / thaw cycle|Stable for 12 months at -20°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

TIRAP also known as Mal is an adapter protein integral to the Toll-like receptor (TLR) signaling pathway. It has a molecular mass of approximately 24 kDa. TIRAP resides mostly in the cytoplasm and gets recruited to the plasma membrane where it assists in signal transduction. It plays a significant role in bridging TLRs such as TLR2 and TLR4 with downstream signaling elements like MyD88. This protein mediates the recruitment of other signaling proteins after TLR activation therefore facilitating the innate immune response.

Biological function summary

TIRAP is part of the innate immune system and contributes to the immune response against pathogens. It forms part of a signaling complex that leads to the activation of NF-kB and MAP kinase pathways resulting in the production of inflammatory cytokines. The protein works by directly interacting with phosphatidylinositol 45-bisphosphate at the plasma membrane through its phosphoinositide-binding motif. This interaction ensures that TIRAP is correctly positioned to relay signals for immune activation.

Pathways

TIRAP functions in the MyD88-dependent signaling pathway which is significant for initiating inflammatory responses. TIRAP and MyD88 work in tandem to propagate signals from activated TLRs. This relationship not only aids in inflammation but also in the differentiation of immune cells. TIRAP is also related to other proteins like IRAK1 in these pathways which further transduces signals leading to downstream activation of transcription factors relevant for immune responses.

TIRAP plays a part in conditions like systemic lupus erythematosus and septic shock. Mutations in the TIRAP gene can alter the signaling cascade leading to aberrant immune responses associated with these diseases. In this context TIRAP interacts with TLR4 and any dysfunction in the interaction may contribute to the excessive inflammation seen in septic shock. Understanding TIRAP's role in these diseases provides insight into targeted therapeutic strategies.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

See full target information TIRAP

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Publications (16)

Recent publications for all applications. Explore the full list and refine your search

Experimental & molecular medicine 56:2588-2601 PubMed39617791

2024

Hypothermic oxygenated perfusion inhibits CLIP1-mediated TIRAP ubiquitination via TFPI2 to reduce ischemia‒reperfusion injury of the fatty liver.

Applications

Unspecified application

Species

Unspecified reactive species

Pengpeng Yue,Xiaoyan Lv,Hankun Cao,Yongkang Zou,Jian You,Jun Luo,Zhongshan Lu,Hao Chen,Zhongzhong Liu,Zibiao Zhong,Yan Xiong,Xiaoli Fan,Qifa Ye

Frontiers in pharmacology 15:1449985 PubMed39263577

2024

Protective effects of insulin on dry eye syndrome via TLR4/NF-κB pathway: based on network pharmacology and experiments validation.

Applications

Unspecified application

Species

Unspecified reactive species

Xiuxiu Yuan,Yu Zhang,Siyi Wang,Zhiyu Du

Frontiers in immunology 14:1150971 PubMed37090722

2023

A non-invasive strategy for suppressing asthmatic airway inflammation and remodeling: Inhalation of nebulized hypoxic hUCMSC-derived extracellular vesicles.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaowei Xu,Ying Wang,Xinkai Luo,Xuerong Gao,Weifeng Gu,Yongbin Ma,Lili Xu,Mengzhu Yu,Xi Liu,Jiameng Liu,Xuefeng Wang,Tingting Zheng,Chaoming Mao,Liyang Dong

Frontiers in microbiology 13:989183 PubMed36171749

2022

Toll-like receptor 2 signaling pathway activation contributes to a highly efficient inflammatory response in Japanese encephalitis virus-infected mouse microglial cells by proteomics.

Applications

Unspecified application

Species

Unspecified reactive species

Guanyu Zhao,Yan Gao,Jiaqi Zhang,He Zhang,Changzhan Xie,Fulong Nan,Sheng Feng,Zhuo Ha,Chenghui Li,Xiangyu Zhu,Zhuoxin Li,Ping Zhang,Ying Zhang,Huijun Lu,Ningyi Jin

Journal of leukocyte biology 112:383-393 PubMed35199885

2022

The phosphoinositide-3-kinase (PI3K)-delta inhibitor seletalisib impairs monocyte-derived dendritic cells maturation, APC function, and promotes their migration to CCR7 and CXCR4 ligands.

Applications

Unspecified application

Species

Unspecified reactive species

Fernanda Scopelliti,Laura Mercurio,Caterina Cattani,Valentina Dimartino,Cristina Albanesi,Gianfranco Costanzo,Concetta Mirisola,Stefania Madonna,Andrea Cavani

Frontiers in oncology 11:755212 PubMed34804944

2021

Establishment of a prognosis Prediction Model Based on Pyroptosis-Related Signatures Associated With the Immune Microenvironment and Molecular Heterogeneity in Clear Cell Renal Cell Carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Aimin Jiang,Jialin Meng,Yewei Bao,Anbang Wang,Wenliang Gong,Xinxin Gan,Jie Wang,Yi Bao,Zhenjie Wu,Juan Lu,Bing Liu,Linhui Wang

Cell death & disease 12:791 PubMed34385421

2021

Toll-like receptor 4 is a master regulator for colorectal cancer growth under high-fat diet by programming cancer metabolism.

Applications

Unspecified application

Species

Unspecified reactive species

Xianjing Hu,Sarwat Fatima,Minting Chen,Keyang Xu,Chunhua Huang,Rui-Hong Gong,Tao Su,Hoi Leong Xavier Wong,Zhaoxiang Bian,Hiu Yee Kwan

International journal of molecular sciences 22: PubMed34361111

2021

Cigarette Smoke Condensate Exposure Induces Receptor for Advanced Glycation End-Products (RAGE)-Dependent Sterile Inflammation in Amniotic Epithelial Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Helena Choltus,Régine Minet-Quinard,Corinne Belville,Julie Durif,Denis Gallot,Loic Blanchon,Vincent Sapin

American journal of translational research 13:24-37 PubMed33527006

2021

A naturally derived small molecule NDSM253 inhibits IKK1 to suppress inflammation response and promote bone healing after fracture.

Applications

Unspecified application

Species

Unspecified reactive species

Liqi Shen,Yun Xiao,Hui Xie,Hongbin Zhao,Tao Luo,Lin Liu,Xuekun Pan

Cell death & disease 11:374 PubMed32415087

2020

BIG1 controls macrophage pro-inflammatory responses through ARF3-mediated PI(4,5)P2 synthesis.

Applications

Unspecified application

Species

Unspecified reactive species

Lixin Liu,Sulin Zhang,Yirui Wang,Weilian Bao,Yile Zhou,Wenzhen Dang,Xu Wang,Haidong Li,Xinyue Cao,Yan You,Hao Fang,Xiaoyan Shen

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-TIRAP antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TIRAP antibody (AB17218)

Western blot - Anti-TIRAP antibody (AB17218)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

Primary Antibodies

Complementary Products

Primary Antibodies

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (16)

Product promise