Anti-Tissue Factor antibody [EPR18160-175] - BSA and Azide free

• ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-Tissue Factor antibody [EPR18160-175] - BSA and Azide free (AB227910)

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab189483).

Immunofluorescent analysis of 100% methanol-fixed, 0.1% TritonX-100 permeabilized NIH/3T3 (mouse embryonic fibroblast) cells labelling Tissue Factor with ab189483 at 1/50 (11.52 µg/ml) dilution, followed by ab150081, Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) preadsorbed antibody at 1/1000 (2 µg/ml) dilution (Green).

Confocal image showing cytoplasmic staining in NIH/3T3 cell line (shown in green). The counterstain was observed in magenta. Nuclear DNA was labelled with DAPI (shown in blue). Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor^® 594) was used to counterstain tubulin at 1/200 (2.5 µg/ml) dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

Secondary antibody only control : Secondary antibody is ab150081, Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) preadsorbed at 1/1000 (2 µg/ml) dilution.

• IP

Supplier Data

Immunoprecipitation - Anti-Tissue Factor antibody [EPR18160-175] - BSA and Azide free (AB227910)

Tissue Factor was immunoprecipitated from 0.35 mg of mouse brain lysate with ab189483 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab189483 at 1/500 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1000 dilution.

Lane 1 : Mouse brain lysate 10 μg (Input).

Lane 2 : ab189483 IP in mouse brain lysate.

Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab1189483 in mouse brain lysate.

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 5 seconds.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab189483).

All lanes:

Immunoprecipitation - Anti-Tissue Factor antibody [EPR18160-175] (<a href='/en-us/products/primary-antibodies/tissue-factor-antibody-epr18160-175-ab189483'>ab189483</a>)

Predicted band size: 33 kDa

Observed band size: 47 kDa

true

• WB

Supplier Data

Western blot - Anti-Tissue Factor antibody [EPR18160-175] - BSA and Azide free (AB227910)

Blocking/Dilution : 5% NFDM/TBST.

Exposure : Lanes 1 and 3 : 3 minutes; Lanes 2 and 4 : 10 seconds.

The observed molecular mass is consistent with the literature (PMID : 7992837).

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab189483).

All lanes:

Western blot - Anti-Tissue Factor antibody [EPR18160-175] (<a href='/en-us/products/primary-antibodies/tissue-factor-antibody-epr18160-175-ab189483'>ab189483</a>) at 1/1000 dilution

Lane 1:

Mouse skin tissue lysate at 20 µg

Lane 2:

Mouse brain tissue lysate at 20 µg

Lanes 3 - 4:

NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 33 kDa

Observed band size: 33 kDa,47 kDa

true