Rabbit Recombinant Monoclonal Tissue Factor antibody. Carrier free. Suitable for ICC/IF, IP, WB and reacts with Mouse samples.
Images
![Western blot - Anti-Tissue Factor antibody [EPR18160-175] - BSA and Azide free (AB227910), expandable thumbnail](https://content.abcam.com/products/images/tissue-factor-antibody-epr18160-175-bsa-and-azide-free-ab227910--western-blot-img59300.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Tissue Factor antibody [EPR18160-175] - BSA and Azide free (AB227910)")
![Immunoprecipitation - Anti-Tissue Factor antibody [EPR18160-175] - BSA and Azide free (AB227910), expandable thumbnail](https://content.abcam.com/products/images/tissue-factor-antibody-epr18160-175-bsa-and-azide-free-ab227910--immunoprecipitation-img642.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-Tissue Factor antibody [EPR18160-175] - BSA and Azide free (AB227910)")
![Immunocytochemistry/ Immunofluorescence - Anti-Tissue Factor antibody [EPR18160-175] - BSA and Azide free (AB227910), expandable thumbnail](https://content.abcam.com/products/images/tissue-factor-antibody-epr18160-175-bsa-and-azide-free-ab227910--immunocytochemistry-immunofluorescence-img440785.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-Tissue Factor antibody [EPR18160-175] - BSA and Azide free (AB227910)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| ICC/IF | IP | WB | |
|---|---|---|---|
| Mouse | Tested | Tested | Tested |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
Associated Products
Select an associated product type
7 products for Alternative Product
Target data
Function
Initiates blood coagulation by forming a complex with circulating factor VII or VIIa. The [TF:VIIa] complex activates factors IX or X by specific limited proteolysis. TF plays a role in normal hemostasis by initiating the cell-surface assembly and propagation of the coagulation protease cascade.
Alternative names
CD142, Cf-3, Cf3, F3, Tissue factor, TF, Coagulation factor III
Recommended products
Rabbit Recombinant Monoclonal Tissue Factor antibody. Carrier free. Suitable for ICC/IF, IP, WB and reacts with Mouse samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR18160-175
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab227910 is the carrier-free version of Anti-Tissue Factor antibody [EPR18160-175] ab189483.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Tissue Factor also known as coagulation factor III or CD142 is an important player in the blood coagulation process. This protein with a molecular mass of approximately 47 kDa is a transmembrane glycoprotein. It is expressed widely in various tissues including the lungs heart and brain and plays an important role in initiating the coagulation cascade when exposed to blood. In endothelial cells tissue factor is generally not present unless these cells are in a state of disturbance supporting its role as a primary defense mechanism against vascular injury.
- Biological function summary
Tissue factor acts as a cofactor for factor VIIa forming a complex that rapidly catalyzes the activation of factor X to factor Xa. This catalytic action ultimately leads to fibrin formation which is essential for clotting. Tissue Factor (TF) is part of the extrinsic pathway of the coagulation cascade and is fundamental in the generation of a hemostatic response to tissue injury. By regulating thrombin generation through its interaction with other coagulation factors tissue factor plays an important role in maintaining hemostatic balance.
- Pathways
Tissue factor is integral in the extrinsic pathway of coagulation which works closely with the intrinsic pathway to ensure effective hemostasis. It complexes with factor VIIa and triggers downstream effects involving proteins like factor X and thrombin. Tissue factor forms a bridge between cellular injury responses and the blood coagulation system linking these two significant physiological processes. The interaction with these proteins highlights its central role in hemostatic pathways and highlights its significance in the coagulation network.
- Associated diseases and disorders
Tissue factor is associated with conditions like thrombosis and cardiovascular diseases where its expression can become dysregulated. Abnormal activation of tissue factor contributes directly to the pathological clot formation seen in thrombosis. It is also implicated in cancer-related coagulopathy where it can facilitate tumor-associated thrombosis by interacting with cancer cell surfaces. Additionally in such diseases the interplay between tissue factor and thrombin becomes important as it affects disease progression and the severity of clot-related complications.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
3 product images
![Western blot - Anti-Tissue Factor antibody [EPR18160-175] - BSA and Azide free (ab227910), expandable thumbnail](https://content.abcam.com/products/images/tissue-factor-antibody-epr18160-175-bsa-and-azide-free-ab227910--western-blot-img59300.jpg "Western blot - Anti-Tissue Factor antibody [EPR18160-175] - BSA and Azide free (ab227910)")
Western blot - Anti-Tissue Factor antibody [EPR18160-175] - BSA and Azide free (ab227910)
Blocking/Dilution: 5% NFDM/TBST.
Exposure: Lanes 1 and 3: 3 minutes; Lanes 2 and 4: 10 seconds.
The observed molecular mass is consistent with the literature (PMID: 7992837).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Tissue Factor antibody [EPR18160-175] ab189483).
All lanes: Western blot - Anti-Tissue Factor antibody [EPR18160-175] (Anti-Tissue Factor antibody [EPR18160-175] ab189483) at 1/1000 dilution
Lane 1: Mouse skin tissue lysate at 20 µg
Lane 2: Mouse brain tissue lysate at 20 µg
Lanes 3 - 4: NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate at 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 33 kDa
Observed band size: 33 kDa, 47 kDa
![Immunoprecipitation - Anti-Tissue Factor antibody [EPR18160-175] - BSA and Azide free (ab227910), expandable thumbnail](https://content.abcam.com/products/images/tissue-factor-antibody-epr18160-175-bsa-and-azide-free-ab227910--immunoprecipitation-img642.jpg "Immunoprecipitation - Anti-Tissue Factor antibody [EPR18160-175] - BSA and Azide free (ab227910)")
Immunoprecipitation - Anti-Tissue Factor antibody [EPR18160-175] - BSA and Azide free (ab227910)
Tissue Factor was immunoprecipitated from 0.35 mg of mouse brain lysate with Anti-Tissue Factor antibody [EPR18160-175] ab189483 at 1/30 dilution. Western blot was performed from the immunoprecipitate using Anti-Tissue Factor antibody [EPR18160-175] ab189483 at 1/500 dilution. VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366), was used for detection at 1/1000 dilution.
Lane 1: Mouse brain lysate 10 μg (Input).
Lane 2: Anti-Tissue Factor antibody [EPR18160-175] ab189483 IP in mouse brain lysate.
Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab1189483 in mouse brain lysate.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 5 seconds.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Tissue Factor antibody [EPR18160-175] ab189483).
All lanes: Immunoprecipitation - Anti-Tissue Factor antibody [EPR18160-175] (Anti-Tissue Factor antibody [EPR18160-175] ab189483)
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 33 kDa
Observed band size: 47 kDa
![Immunocytochemistry/ Immunofluorescence - Anti-Tissue Factor antibody [EPR18160-175] - BSA and Azide free (ab227910), expandable thumbnail](https://content.abcam.com/products/images/tissue-factor-antibody-epr18160-175-bsa-and-azide-free-ab227910--immunocytochemistry-immunofluorescence-img440785.jpg "Immunocytochemistry/ Immunofluorescence - Anti-Tissue Factor antibody [EPR18160-175] - BSA and Azide free (ab227910)")
Immunocytochemistry/ Immunofluorescence - Anti-Tissue Factor antibody [EPR18160-175] - BSA and Azide free (ab227910)
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Tissue Factor antibody [EPR18160-175] ab189483).
Immunofluorescent analysis of 100% methanol-fixed, 0.1% TritonX-100 permeabilized NIH/3T3 (mouse embryonic fibroblast) cells labelling Tissue Factor with Anti-Tissue Factor antibody [EPR18160-175] ab189483 at 1/50 (11.52 µg/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 (2 µg/ml) dilution (Green).
Confocal image showing cytoplasmic staining in NIH/3T3 cell line (shown in green). The counterstain was observed in magenta. Nuclear DNA was labelled with DAPI (shown in blue). Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 (2.5 µg/ml) dilution (Magenta). The Nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 µg/ml) dilution.
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com