Rabbit Recombinant Monoclonal Tissue Factor antibody. Suitable for IP, Flow Cyt, WB, ICC/IF, IHC-P and reacts with Human samples. Cited in 10 publications.
IgG
Rabbit
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Liquid
Monoclonal
IP | Flow Cyt | WB | ICC/IF | IHC-P | |
---|---|---|---|---|---|
Human | Tested | Tested | Tested | Tested | Tested |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 1/30 | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 1/500 | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 1/1000 | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 1/100 | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 1/500 | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Initiates blood coagulation by forming a complex with circulating factor VII or VIIa. The [TF:VIIa] complex activates factors IX or X by specific limited proteolysis. TF plays a role in normal hemostasis by initiating the cell-surface assembly and propagation of the coagulation protease cascade.
Tissue factor, TF, Coagulation factor III, Thromboplastin, F3
Rabbit Recombinant Monoclonal Tissue Factor antibody. Suitable for IP, Flow Cyt, WB, ICC/IF, IHC-P and reacts with Human samples. Cited in 10 publications.
IgG
Rabbit
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Liquid
Monoclonal
EPR22548-232
Affinity purification Protein A
Blue Ice
1-2 weeks
+4°C
-20°C
Upon delivery aliquot
Avoid freeze / thaw cycle
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
For more information, read more on recombinant antibodies.
This supplementary information is collated from multiple sources and compiled automatically.
Tissue Factor also known as coagulation factor III or CD142 is an important player in the blood coagulation process. This protein with a molecular mass of approximately 47 kDa is a transmembrane glycoprotein. It is expressed widely in various tissues including the lungs heart and brain and plays an important role in initiating the coagulation cascade when exposed to blood. In endothelial cells tissue factor is generally not present unless these cells are in a state of disturbance supporting its role as a primary defense mechanism against vascular injury.
Tissue factor acts as a cofactor for factor VIIa forming a complex that rapidly catalyzes the activation of factor X to factor Xa. This catalytic action ultimately leads to fibrin formation which is essential for clotting. Tissue Factor (TF) is part of the extrinsic pathway of the coagulation cascade and is fundamental in the generation of a hemostatic response to tissue injury. By regulating thrombin generation through its interaction with other coagulation factors tissue factor plays an important role in maintaining hemostatic balance.
Tissue factor is integral in the extrinsic pathway of coagulation which works closely with the intrinsic pathway to ensure effective hemostasis. It complexes with factor VIIa and triggers downstream effects involving proteins like factor X and thrombin. Tissue factor forms a bridge between cellular injury responses and the blood coagulation system linking these two significant physiological processes. The interaction with these proteins highlights its central role in hemostatic pathways and highlights its significance in the coagulation network.
Tissue factor is associated with conditions like thrombosis and cardiovascular diseases where its expression can become dysregulated. Abnormal activation of tissue factor contributes directly to the pathological clot formation seen in thrombosis. It is also implicated in cancer-related coagulopathy where it can facilitate tumor-associated thrombosis by interacting with cancer cell surfaces. Additionally in such diseases the interplay between tissue factor and thrombin becomes important as it affects disease progression and the severity of clot-related complications.
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This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
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Immunohistochemical analysis of paraffin-embedded human kidney tissue labeling Tissue Factor with ab228968 at 1/500 dilution, followed by ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Positive staining in human renal glomerulus (PMID: 7684196). The section was incubated with ab228968 for 30 mins at RT. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
Perform heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20mins.
Flow cytometric analysis of MCF7 (human breast adenocarcinoma epithelial cell, Left) / A431 (human epidermoid carcinoma epithelial cell, Right) labeling Tissue Factor with ab228968 at 1/500 (red) compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077), at 1/2000 dilution was used as the secondary antibody.
Low expression: MCF7 (PMID: 24137414, PMID: 28938620).
Gated on viable cells.
ab228968 was shown to specifically react with Tissue Factor in wild-type HAP1 cells as signal was lost in Tissue Factor knockout cells. Wild-type and Tissue Factor knockout samples were subjected to SDS-PAGE. ab228968 and Anti-GAPDH antibody [EPR16891] - Loading Control ab181602 (Rabbit anti-GAPDH loading control) were incubated 1 hour at room temperature at 1/1000 dilution and 1/200,000 dilution respectively. Blots were developed with Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (Goat Anti-Rabbit IgG H&L (HRP) ab97051) secondary antibody at 1/100,000 dilution for 1 hour at room temperature before imaging. The blot was developed on a BIO-RAD® ChemiDoc™ MP instrument using the ECL technique.
The molecular weight observed is consistent with what has been described in the literature (PMID: 28938620).
Low expression: MCF-7 (PMID: 24137414, 28938620).
Exposure times: Lanes 1-2: 70 secs; Lanes 3-6: 3.25 secs.
Blocking/Dilution buffer: 5% NFDM/TBST.
All lanes: Western blot - Anti-Tissue Factor antibody [EPR22548-232] (ab228968) at 1/1000 dilution
Lane 1: Wild-type HAP1 whole cell lysate at 60 µg
Lane 2: Tissue Factor knockout HAP1 whole cell lysate at 60 µg
Lane 3: A431 (human epidermoid carcinoma epithelial cell), whole cell lysate at 20 µg
Lane 4: MDA-MB-231 (human breast adenocarcinoma epithelial cell), whole cell lysate at 20 µg
Lane 5: MCF7 (human breast adenocarcinoma epithelial cell), whole cell lysate at 20 µg
Lane 6: BxPC-3 (human pancreas adenocarcinoma epithelial cell), whole cell lysate at 20 µg
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Predicted band size: 33 kDa
Observed band size: 47 kDa
ab228968 was shown to specifically react with Tissue Factor in wild-type HAP1 cells as signal was lost in Tissue Factor knockout cells. Wild-type and Tissue Factor knockout samples were subjected to SDS-PAGE. ab228968 and Anti-GAPDH antibody [EPR16891] - Loading Control ab181602 (Rabbit anti-GAPDH loading control) were incubated 1 hour at room temperature at 1/1000 dilution and 1/200,000 dilution respectively. Blots were developed with Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (Goat Anti-Rabbit IgG H&L (HRP) ab97051) secondary antibody at 1/100,000 dilution for 1 hour at room temperature before imaging. The blot was developed on a BIO-RAD® ChemiDoc™ MP instrument using the ECL technique.
The molecular weight observed is consistent with what has been described in the literature (PMID: 28938620).
Low expression: MCF-7 (PMID: 24137414, 28938620).
Exposure times: Lanes 1-2: 70 secs; Lanes 3-6: 3.25 secs.
Blocking/Dilution buffer: 5% NFDM/TBST.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab228968).
Immunohistochemical analysis of paraffin-embedded human cervix carcinoma tissue labeling Tissue Factor with ab228968 at 1/500 dilution, followed by ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Membranous and weak cytoplasmic staining on tumor cells of human cervix carcinoma (PMID: 26383146). The section was incubated with ab228968 for 30 mins at RT. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
Perform heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20mins.
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilizedA431 (human epidermoid carcinoma epithelial cell) and MCF7 () cells labeling Tissue Factor with ab228968 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing membranous staining in A431 cell line. Low expression: MCF7(PMID: 24137414, 28938620. The nuclear stain is DAPI (blue).
Counterstained with Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) at a 1/200 dilution (red).
The negative control is the secondary antibody only.
Tissue Factor was immunoprecipitated from 0.35 mg A431 (human epidermoid carcinoma epithelial cell) whole cell lysate with ab228968 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab228968 at 1/1000 dilution. VeriBlot for IP for detection (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366), was used Detection Reagent at 1/5000 dilution.
Lane 1: A431 whole cell lysate 10 μg (Input).
Lane 2: ab228968 IP in A431 whole cell lysate.
Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab228968 in A431 whole cell lysate.
Blocking/Dilution buffer: 5% NFDM/TBST.
Exposure time: 30 seconds.
All lanes: Immunoprecipitation - Anti-Tissue Factor antibody [EPR22548-232] (ab228968)
Predicted band size: 33 kDa
Observed band size: 47 kDa
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