Anti-Tissue Factor antibody [EPR22548-240]

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tissue Factor antibody [EPR22548-240] (AB252918)

Immunohistochemical analysis of paraffin-embedded human kidney tissue labeling Tissue Factor with ab252918 at 1/2000 dilution, followed by Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining in human renal glomerulus (PMID : 7684196) is observed. Counter stained with hematoxylin.
Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 minutes.

The section was incubated with ab252918 for 30 minutes at RT.

The immunostaining was performed on a Leica Biosystems BOND^® RX instrument.

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tissue Factor antibody [EPR22548-240] (AB252918)

Immunohistochemical analysis of paraffin-embedded human cervix carcinoma tissue labeling Tissue Factor with ab252918 at 1/2000 dilution, followed by Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Membranous and weak cytoplasmic staining on tumor cells of human cervix carcinoma (PMID : 26383146) is observed. Counter stained with hematoxylin.
Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 minutes.

The section was incubated with ab252918 for 30 minutess at RT.

The immunostaining was performed on a Leica Biosystems BOND^® RX instrument.

• mIHC

Lab

Multiplex immunohistochemistry - Anti-Tissue Factor antibody [EPR22548-240] (AB252918)

Fluorescence multiplex immunohistochemical analysis of paraffin-embedded Human kidney tissue. Panel A : Merged staining of anti-Hexokinase 1 (gray; Opal™690), anti-Angiotensin Converting Enzyme 1 (green; Opal™520) and anti-Tissue Factor (red; Opal™570) on human kidney. Panel B : Anti-Tissue Factor stained on renal glomeruli. Panel C : Anti-Angiotensin Converting Enzyme 1 stained on proximal tubules. Panel D : Anti-Hexokinase 1 stained on distal tubules. The section was incubated in three rounds of staining : in the order of ab150423, ab254222, and ab252918 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system. The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins. Counterstained with DAPI.

• WB

Unknown

Western blot - Anti-Tissue Factor antibody [EPR22548-240] (AB252918)

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-Tissue Factor antibody [EPR22548-240] (ab252918) at 1/1000 dilution

Lane 1:

Wild-type HAP1 whole cell lysate at 60 µg

Lane 2:

Tissue Factor knockout HAP1 whole cell lysate at 60 µg

Lane 3:

A431 (human epidermoid carcinoma cell line) whole cell lysate at 20 µg

Lane 4:

MDA-MB-231 (human breast adenocarcinoma cell line) whole cell lysate at 20 µg

Lane 5:

MCF7 (human breast adenocarcinoma cell line) whole cell lysate at 20 µg

Lane 6:

BxPC-3 (human pancreas adenocarcinoma epithelial cell line) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 33 kDa

Observed band size: 47 kDa

false

• WB

Unknown

Western blot - Anti-Tissue Factor antibody [EPR22548-240] (AB252918)

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-Tissue Factor antibody [EPR22548-240] (ab252918) at 1/1000 dilution

Lane 1:

Human brain cell lysate at 20 µg

Lane 2:

Human heart cell lysate at 20 µg

Lane 3:

Human fetal kidney cell lysate at 20 µg

Secondary

All lanes:

Western blot - VeriBlot for IP Detection Reagent (HRP) (<a href='/en-us/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/1000 dilution

Predicted band size: 33 kDa

Observed band size: 47 kDa

false

Exposure time: 8s