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Rabbit Recombinant Monoclonal Tissue Factor antibody. Suitable for mIHC, WB, IHC-P and reacts with Human samples. Cited in 2 publications.

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Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tissue Factor antibody [EPR22548-240] (AB252918), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tissue Factor antibody [EPR22548-240] (AB252918), expandable thumbnail
  • Western blot - Anti-Tissue Factor antibody [EPR22548-240] (AB252918), expandable thumbnail
  • Western blot - Anti-Tissue Factor antibody [EPR22548-240] (AB252918), expandable thumbnail
  • Multiplex immunohistochemistry - Anti-Tissue Factor antibody [EPR22548-240] (AB252918), expandable thumbnail

Publications

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
mIHCIPFlow CytWBICC/IFIHC-P
Human
Tested
Not recommended
Not recommended
Tested
Not recommended
Tested

Tested
Tested

Species

Human

Dilution info

1/2000

Notes

-

Not recommended
Not recommended

Species

Human

Dilution info

-

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species

Human

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

1/1000

Notes

-

Not recommended
Not recommended

Species

Human

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

1/2000

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Target data

Function

Initiates blood coagulation by forming a complex with circulating factor VII or VIIa. The [TF:VIIa] complex activates factors IX or X by specific limited proteolysis. TF plays a role in normal hemostasis by initiating the cell-surface assembly and propagation of the coagulation protease cascade.

Alternative names

Tissue factor, TF, Coagulation factor III, Thromboplastin, F3

Recommended products

Rabbit Recombinant Monoclonal Tissue Factor antibody. Suitable for mIHC, WB, IHC-P and reacts with Human samples. Cited in 2 publications.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number

EPR22548-240

Purification technique

Affinity purification Protein A

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

Tissue Factor also known as coagulation factor III or CD142 is an important player in the blood coagulation process. This protein with a molecular mass of approximately 47 kDa is a transmembrane glycoprotein. It is expressed widely in various tissues including the lungs heart and brain and plays an important role in initiating the coagulation cascade when exposed to blood. In endothelial cells tissue factor is generally not present unless these cells are in a state of disturbance supporting its role as a primary defense mechanism against vascular injury.

Biological function summary

Tissue factor acts as a cofactor for factor VIIa forming a complex that rapidly catalyzes the activation of factor X to factor Xa. This catalytic action ultimately leads to fibrin formation which is essential for clotting. Tissue Factor (TF) is part of the extrinsic pathway of the coagulation cascade and is fundamental in the generation of a hemostatic response to tissue injury. By regulating thrombin generation through its interaction with other coagulation factors tissue factor plays an important role in maintaining hemostatic balance.

Pathways

Tissue factor is integral in the extrinsic pathway of coagulation which works closely with the intrinsic pathway to ensure effective hemostasis. It complexes with factor VIIa and triggers downstream effects involving proteins like factor X and thrombin. Tissue factor forms a bridge between cellular injury responses and the blood coagulation system linking these two significant physiological processes. The interaction with these proteins highlights its central role in hemostatic pathways and highlights its significance in the coagulation network.

Associated diseases and disorders

Tissue factor is associated with conditions like thrombosis and cardiovascular diseases where its expression can become dysregulated. Abnormal activation of tissue factor contributes directly to the pathological clot formation seen in thrombosis. It is also implicated in cancer-related coagulopathy where it can facilitate tumor-associated thrombosis by interacting with cancer cell surfaces. Additionally in such diseases the interplay between tissue factor and thrombin becomes important as it affects disease progression and the severity of clot-related complications.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

5 product images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tissue Factor antibody [EPR22548-240] (ab252918), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tissue Factor antibody [EPR22548-240] (ab252918)

    Immunohistochemical analysis of paraffin-embedded human cervix carcinoma tissue labeling Tissue Factor with ab252918 at 1/2000 dilution, followed by Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Membranous and weak cytoplasmic staining on tumor cells of human cervix carcinoma (PMID: 26383146) is observed. Counter stained with hematoxylin.
    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 minutes.

    The section was incubated with ab252918 for 30 minutess at RT.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tissue Factor antibody [EPR22548-240] (ab252918), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tissue Factor antibody [EPR22548-240] (ab252918)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue labeling Tissue Factor with ab252918 at 1/2000 dilution, followed by Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Positive staining in human renal glomerulus (PMID: 7684196) is observed. Counter stained with hematoxylin.
    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 minutes.

    The section was incubated with ab252918 for 30 minutes at RT.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

  • Western blot - Anti-Tissue Factor antibody [EPR22548-240] (ab252918), expandable thumbnail

    Western blot - Anti-Tissue Factor antibody [EPR22548-240] (ab252918)

    Blocking and dilution buffer: 5% NFDM/TBST.

    All lanes: Western blot - Anti-Tissue Factor antibody [EPR22548-240] (ab252918) at 1/1000 dilution

    Lane 1: Wild-type HAP1 whole cell lysate at 60 µg

    Lane 2: Tissue Factor knockout HAP1 whole cell lysate at 60 µg

    Lane 3: A431 (human epidermoid carcinoma cell line) whole cell lysate at 20 µg

    Lane 4: MDA-MB-231 (human breast adenocarcinoma cell line) whole cell lysate at 20 µg

    Lane 5: MCF7 (human breast adenocarcinoma cell line) whole cell lysate at 20 µg

    Lane 6: BxPC-3 (human pancreas adenocarcinoma epithelial cell line) whole cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Predicted band size: 33 kDa

    Observed band size: 47 kDa

  • Western blot - Anti-Tissue Factor antibody [EPR22548-240] (ab252918), expandable thumbnail

    Western blot - Anti-Tissue Factor antibody [EPR22548-240] (ab252918)

    Blocking and dilution buffer: 5% NFDM/TBST.

    All lanes: Western blot - Anti-Tissue Factor antibody [EPR22548-240] (ab252918) at 1/1000 dilution

    Lane 1: Human brain cell lysate at 20 µg

    Lane 2: Human heart cell lysate at 20 µg

    Lane 3: Human fetal kidney cell lysate at 20 µg

    Secondary

    All lanes: Western blot - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/1000 dilution

    Predicted band size: 33 kDa

    Observed band size: 47 kDa

    Exposure time: 8s

  • Multiplex immunohistochemistry - Anti-Tissue Factor antibody [EPR22548-240] (ab252918), expandable thumbnail

    Multiplex immunohistochemistry - Anti-Tissue Factor antibody [EPR22548-240] (ab252918)

    Fluorescence multiplex immunohistochemical analysis of paraffin-embedded Human kidney tissue.

    Panel A: Merged staining of anti-Hexokinase 1 (gray; Opal™690), anti-Angiotensin Converting Enzyme 1 (green; Opal™520) and anti-Tissue Factor (red; Opal™570) on human kidney.
    Panel B: Anti-Tissue Factor stained on renal glomeruli.
    Panel C: Anti-Angiotensin Converting Enzyme 1 stained on proximal tubules.
    Panel D: Anti-Hexokinase 1 stained on distal tubules.

    The section was incubated in three rounds of staining: in the order of Anti-Hexokinase 1 antibody [EPR10134(B)] - Mitochondrial Outer Membrane Marker ab150423, Anti-Angiotensin Converting Enzyme 1 antibody [EPR22291-247] ab254222, and ab252918 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system. The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins. Counterstained with DAPI.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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