Anti-Tissue kallikrein antibody

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tissue kallikrein antibody (AB28289)

IHC image of ab28289 staining in human normal pancreas formalin fixed paraffin embedded tissue section, performed on a Leica Bond^TM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with EDTA (pH9, epitope retrieval solution 2) for 20 mins. The section was then incubated with ab28289, 1μg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

• WB

Supplier Data

Western blot - Anti-Tissue kallikrein antibody (AB28289)

All lanes:

Western blot - Anti-Tissue kallikrein antibody (ab28289) at 1/1000 dilution

Lane 3:

Kallikrein-1 (rhK-1) at 0.05 µg

Lane 4:

Kallikrein-1 (rhK-1) at 0.01 µg

Lane 5:

Kallikrein-1 (rhK-1) at 0.001 µg

Lane 7:

Kallikrein-3 (rhK-3) at 0.05 µg

Lane 8:

Kallikrein-3 (rhK-3) at 0.01 µg

Lane 9:

Kallikrein-3 (rhK-3) at 0.001 µg

Lane 11:

Kallikrein-4 (rhK-4) at 0.05 µg

Lane 12:

Kallikrein-4 (rhK-4) at 0.01 µg

Lane 13:

Kallikrein-4 (rhK-4) at 0.001 µg

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