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AB323796

Anti-Tissue kallikrein antibody [EPR25709-526]

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Rabbit Recombinant Monoclonal Tissue kallikrein antibody. Suitable for WB, Dot and reacts with Human, Recombinant fragment - Human samples.

View Alternative Names

Kallikrein-1, Kidney/pancreas/salivary gland kallikrein, Tissue kallikrein, KLK1

3 Images
Western blot - Anti-Tissue kallikrein antibody [EPR25709-526] (AB323796)
  • WB

Supplier Data

Western blot - Anti-Tissue kallikrein antibody [EPR25709-526] (AB323796)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

The identity of the bands lower than 40 kDa are predicted to be glycosylated isoforms.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

All lanes:

Western blot - Anti-Tissue kallikrein antibody [EPR25709-526] (ab323796) at 1/1000 dilution

Lane 1:

Untreated human salivary gland tissue lysate at 20 µg

Lane 2:

Human salivary gland tissue lysate treated with Protein Deglycosylation MIX II at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 40 kDa,29 kDa,36 kDa

false

Exposure time: 180s

Western blot - Anti-Tissue kallikrein antibody [EPR25709-526] (AB323796)
  • WB

Supplier Data

Western blot - Anti-Tissue kallikrein antibody [EPR25709-526] (AB323796)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Low expression : Human lung and testis.

The identity of the bands lower than 40 kDa are predicted to be glycosylated isoforms.

The identity of the bands higher than 70 kDa is unkown.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

All lanes:

Western blot - Anti-Tissue kallikrein antibody [EPR25709-526] (ab323796) at 1/1000 dilution

Lane 1:

Human salivary gland tissue lysate at 20 µg

Lane 2:

Human pancreas tissue lysate at 20 µg

Lane 3:

Human lung tissue lysate at 20 µg

Lane 4:

Human testis tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 40 kDa,36 kDa

false

Exposure time: 81s

Dot Blot - Anti-Tissue kallikrein antibody [EPR25709-526] (AB323796)
  • Dot

Supplier Data

Dot Blot - Anti-Tissue kallikrein antibody [EPR25709-526] (AB323796)

Dot blot analysis of Tissue kallikrein using ab323796 at 1 : 1000 (0.49 ug/ml) followed by a Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1 : 100,000 dilution.

Lane1 : LIF-tagged human KLK1 fragment
Lane2 : LIF-tagged human KLK2 fragment
Lane3 : LIF-tagged human KLK3 fragment

Exposure time : 180 seconds.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

In Dot Blot, Anti-LIF antibody [EPR21713-25] - BSA and Azide free (Capture) (ab244795) staining at 1 : 1000 dilution.

All lanes:

Dot Blot - Anti-Tissue kallikrein antibody [EPR25709-526] (ab323796) at 1/1000 dilution

Lane 1:

LIF-tagged human KLK1 fragment

Lane 2:

LIF-tagged human KLK2 fragment

Lane 3:

LIF-tagged human KLK3 fragment

Secondary

All lanes:

Dot Blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

false

Exposure time: 180s

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR25709-526

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, Dot

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

Unsuitable for human cell lines.

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Complementary Products

Assay Kits

AB119598

Human Kallikrein 1 ELISA Kit (KLK1)

Sandwich ELISA - Human Kallikrein 1 ELISA Kit (KLK1) (AB119598)

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  • 0
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "Dot" : {"fullname" : "Dot Blot", "shortname":"Dot"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "Dot-species-checked": "guaranteed", "Dot-species-dilution-info": "", "Dot-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "Dot-species-checked": "notRecommended", "Dot-species-dilution-info": "", "Dot-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Recombinant fragment - Human": { "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "Dot-species-checked": "testedAndGuaranteed", "Dot-species-dilution-info": "1/1000", "Dot-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Glandular kallikreins cleave Met-Lys and Arg-Ser bonds in kininogen to release Lys-bradykinin.. (Microbial infection) Cleaves Neisseria meningitidis NHBA in saliva; Neisseria is an obligate commensal of the nasopharyngeal mucosa.
See full target information KLK1
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com