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AB62763

Anti-Tissue Plasminogen Activator antibody

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(3 Publications)

Rabbit Polyclonal Tissue Plasminogen Activator antibody. Suitable for ELISA, WB and reacts with Mouse, Rat samples. Cited in 3 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Mouse Plat.

View Alternative Names

Tissue-type plasminogen activator, t-PA, t-plasminogen activator, tPA, Plat

2 Images
Western blot - Anti-Tissue Plasminogen Activator antibody (AB62763)
  • WB

Unknown

Western blot - Anti-Tissue Plasminogen Activator antibody (AB62763)

All lanes:

Western blot - Anti-Tissue Plasminogen Activator antibody (ab62763) at 2 µg/mL

Lane 1:

Mouse TPA Tissue Plasminogen Activator (non-reduced) at 0.5 µg

Lane 2:

Mouse TPA Tissue Plasminogen Activator + 3 µg CPAI (non-reduced) at 0.5 µg

Lane 3:

Mouse TPA Tissue Plasminogen Activator (reduced) at 0.5 µg

Lane 4:

Mouse TPA Tissue Plasminogen Activator + 3ug CPAI (reduced) at 0.5 µg

Lane 5:

Marker

Secondary

All lanes:

Anti Rabbit IgG ABC Kit

Predicted band size: 63 kDa

Observed band size: 100 kDa,125 kDa,38 kDa,75 kDa,85 kDa

false

Western blot - Anti-Tissue Plasminogen Activator antibody (AB62763)
  • WB

Unknown

Western blot - Anti-Tissue Plasminogen Activator antibody (AB62763)

All lanes:

Western blot - Anti-Tissue Plasminogen Activator antibody (ab62763)

Predicted band size: 63 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Rat

Applications

WB, ELISA

applications

Immunogen

Recombinant Full Length Protein corresponding to Mouse Plat.

P11214

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ELISA" : {"fullname" : "ELISA", "shortname":"ELISA"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1-5 µg/mL", "WB-species-notes": "<p></p>" }, "Rat": { "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "<p></p>", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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Product details

The concentration of this antibody is lot specific.
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
Constituents: 0.82% Sodium phosphate, 0.58% Sodium chloride, 0.0292% EDTA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-80°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Tissue Plasminogen Activator (tPA) also known as a plasminogen activator acts mechanically to convert plasminogen to plasmin a serine protease. This reaction occurs at the surface of a fibrin clot leading to clot degradation a process known as fibrinolysis. tPA has a molecular mass of approximately 70 kDa. It is mainly expressed in vascular endothelial cells and is released into the bloodstream in response to stimuli such as circulatory stasis or endothelial damage.
Biological function summary

TPA plays a critical role in thrombolysis by breaking down blood clots into their soluble components. It regulates plasminogen function by cleaving this zymogen to yield the active protease plasmin. This function makes tPA integral in maintaining hemostasis and it does not form a part of a larger protein complex. The activity and the regulation of tPA are important for preventing pathologic clotting which can lead to cardiovascular complications.

Pathways

TPA is central to the fibrinolytic pathway. This pathway facilitates the conversion of plasminogen to plasmin enabling clot resolution. In addition tPA interacts with other proteins such as urokinase plasminogen activator (uPA) and inhibitors like plasminogen activator inhibitor-1 (PAI-1). The balance between tPA and its inhibitors is important for the regulation of fibrinolytic activity impacting hemostatic and thrombotic events.

TPA connects closely with conditions like stroke and myocardial infarction due to its thrombolytic properties. In ischemic stroke excessive or insufficient tPA activity can disrupt normal blood flow leading to tissue damage. Additionally in myocardial infarction tPA's role in breaking down clots proves important for restoring coronary blood flow. It is also linked with proteins like fibrinogen as they serve as substrates in the clot degradation process and with PAI-1 which modulates its activity and influences disease progression.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Converts the abundant, but inactive, zymogen plasminogen to plasmin by hydrolyzing a single Arg-Val bond in plasminogen. By controlling plasmin-mediated proteolysis, it plays an important role in tissue remodeling and degradation, in cell migration and many other physiopathological events. During oocyte activation, plays a role in cortical granule reaction in the zona reaction, which contributes to the block to polyspermy (By similarity).
See full target information Plat
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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in cellular neuroscience 13:164 PubMed31105531

2019

Post-synaptic Release of the Neuronal Tissue-Type Plasminogen Activator (tPA).

Applications

Unspecified application

Species

Unspecified reactive species

Sophie Lenoir,Alexandre Varangot,Laurent Lebouvier,Thierry Galli,Yannick Hommet,Denis Vivien

Journal of comparative physiology. B, Biochemical, systemic, and environmental physiology 189:167-177 PubMed30317383

2018

Cardiovascular resistance to thrombosis in 13-lined ground squirrels.

Applications

Unspecified application

Species

Unspecified reactive species

Alison Bonis,Leah Anderson,Gaëlle Talhouarne,Emily Schueller,Jenna Unke,Catherine Krus,Jordan Stokka,Anna Koepke,Brittany Lehrer,Anthony Schuh,Jeremiah J Andersen,Scott Cooper

The Journal of biological chemistry 288:15154-66 PubMed23580652

2013

A lipoprotein receptor cluster IV mutant preferentially binds amyloid-β and regulates its clearance from the mouse brain.

Applications

Unspecified application

Species

Unspecified reactive species

Abhay P Sagare,Robert D Bell,Alaka Srivastava,Jesse D Sengillo,Itender Singh,Yoichiro Nishida,Nienwen Chow,Berislav V Zlokovic
View all publications
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