Name: Anti-Tissue Plasminogen Activator antibody [EPR7232(2)]
SKU: ab157469
Rating: 3 (1 reviews)

Rabbit Recombinant Monoclonal Tissue Plasminogen Activator antibody. Suitable for WB, IHC-P and reacts with Mouse, Rat, Human samples. Cited in 10 publications.

Images

Western blot - Anti-Tissue Plasminogen Activator antibody [EPR7232(2)] (AB157469), expandable thumbnail

Publications

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Form: Liquid
Clonality: Monoclonal

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application

Product promiseTestedExpectedPredictedNot recommended

	IP	WB	IHC-P
Human	Not recommended	Tested	Tested
Mouse	Not recommended	Tested	Tested
Rat	Not recommended	Tested	Tested

Not recommended
Not recommended

Species	Dilution info	Notes
Species Mouse, Rat, Human	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info 1/1000 - 1/5000	Notes -
Species Rat	Dilution info 1/1000 - 1/5000	Notes -
Species Human	Dilution info 1/1000 - 1/5000	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info 1/100 - 1/1000	Notes Perform heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0).
Species Rat	Dilution info 1/100 - 1/1000	Notes Perform heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0).
Species Human	Dilution info 1/100 - 1/1000	Notes Perform heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0).

Associated Products

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Target data

See full target information PLAT

Function

Converts the abundant, but inactive, zymogen plasminogen to plasmin by hydrolyzing a single Arg-Val bond in plasminogen. By controlling plasmin-mediated proteolysis, it plays an important role in tissue remodeling and degradation, in cell migration and many other physiopathological events. During oocyte activation, plays a role in cortical granule reaction in the zona reaction, which contributes to the block to polyspermy (By similarity).

Alternative names

Tissue-type plasminogen activator, t-PA, t-plasminogen activator, tPA, PLAT

Recommended products

Rabbit Recombinant Monoclonal Tissue Plasminogen Activator antibody. Suitable for WB, IHC-P and reacts with Mouse, Rat, Human samples. Cited in 10 publications.

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Form: Liquid
Clonality: Monoclonal
Immunogen: The exact immunogen used to generate this antibody is proprietary information.
Clone number: EPR7232(2)
Purification technique: Affinity purification Protein A
Concentration

Storage

Shipped at conditions: Blue Ice
Appropriate short-term storage duration: 1-2 weeks
Appropriate short-term storage conditions: +4°C
Appropriate long-term storage conditions: -20°C
Aliquoting information: Upon delivery aliquot
Storage information: Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

Tissue Plasminogen Activator (tPA) also known as a plasminogen activator acts mechanically to convert plasminogen to plasmin a serine protease. This reaction occurs at the surface of a fibrin clot leading to clot degradation a process known as fibrinolysis. tPA has a molecular mass of approximately 70 kDa. It is mainly expressed in vascular endothelial cells and is released into the bloodstream in response to stimuli such as circulatory stasis or endothelial damage.

Biological function summary

TPA plays a critical role in thrombolysis by breaking down blood clots into their soluble components. It regulates plasminogen function by cleaving this zymogen to yield the active protease plasmin. This function makes tPA integral in maintaining hemostasis and it does not form a part of a larger protein complex. The activity and the regulation of tPA are important for preventing pathologic clotting which can lead to cardiovascular complications.

Pathways

TPA is central to the fibrinolytic pathway. This pathway facilitates the conversion of plasminogen to plasmin enabling clot resolution. In addition tPA interacts with other proteins such as urokinase plasminogen activator (uPA) and inhibitors like plasminogen activator inhibitor-1 (PAI-1). The balance between tPA and its inhibitors is important for the regulation of fibrinolytic activity impacting hemostatic and thrombotic events.

Associated diseases and disorders

TPA connects closely with conditions like stroke and myocardial infarction due to its thrombolytic properties. In ischemic stroke excessive or insufficient tPA activity can disrupt normal blood flow leading to tissue damage. Additionally in myocardial infarction tPA's role in breaking down clots proves important for restoring coronary blood flow. It is also linked with proteins like fibrinogen as they serve as substrates in the clot degradation process and with PAI-1 which modulates its activity and influences disease progression.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

11 product images

Western blot - Anti-Tissue Plasminogen Activator antibody [EPR7232(2)] (ab157469)
T-PA is serected and mainly released into blood and extracellular fluid to activate plasminogen. The molecular weight observed is consistent with what has been described in PMID: 22069469
All lanes: Western blot - Anti-Tissue Plasminogen Activator antibody [EPR7232(2)] (ab157469) at 1/1000 dilution
Lane 1: Mouse plasma lysates at 15 µg
Lane 2: Rat plasma lysates at 15 µg
Lane 3: Human plasma lysates at 15 µg
Lane 4: Human brain lysates at 15 µg
Lane 5: Human heart lysates at 15 µg
Lane 6: Human liver lysates at 15 µg
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Predicted band size: 63 kDa
Observed band size: 68 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tissue Plasminogen Activator antibody [EPR7232(2)] (ab157469)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat liver tissue sections labeling TPA Tissue Plasminogen Activator with purified ab157469 at 1/1000 dilution (1.91 μg/ml). Heat mediated antigen retrieval was performed using Bond™ Epitope Retrieval Solution 2 (pH 9.0). Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tissue Plasminogen Activator antibody [EPR7232(2)] (ab157469)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse liver tissue sections labeling TPA Tissue Plasminogen Activator with purified ab157469 at 1/1000 dilution (1.91 μg/ml). Heat mediated antigen retrieval was performed using Bond™ Epitope Retrieval Solution 2 (pH 9.0). Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tissue Plasminogen Activator antibody [EPR7232(2)] (ab157469)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human lung carcinoma tissue sections labeling TPA Tissue Plasminogen Activator with purified ab157469 at 1/1000 dilution (1.91 μg/ml). Heat mediated antigen retrieval was performed using Bond™ Epitope Retrieval Solution 2 (pH 9.0). Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tissue Plasminogen Activator antibody [EPR7232(2)] (ab157469)
ab157469 (unpurified) showing +ve staining in Human normal liver.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tissue Plasminogen Activator antibody [EPR7232(2)] (ab157469)
ab157469 (unpurified) showing +ve staining in Human normal kidney.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tissue Plasminogen Activator antibody [EPR7232(2)] (ab157469)
ab157469 (unpurified) showing +ve staining in Human normal brain.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tissue Plasminogen Activator antibody [EPR7232(2)] (ab157469)
ab157469 (unpurified) showing +ve staining in Human skeletal muscle.
Western blot - Anti-Tissue Plasminogen Activator antibody [EPR7232(2)] (ab157469)
All lanes: Western blot - Anti-Tissue Plasminogen Activator antibody [EPR7232(2)] (ab157469) at 1/1000 dilution
All lanes: Human plasma lysate at 10 µg
Secondary
All lanes: HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 63 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tissue Plasminogen Activator antibody [EPR7232(2)] (ab157469)
Immunohistochemical analysis of paraffin-embedded Human cervial carcinoma tissue labeling TPA Tissue Plasminogen Activator with ab157469 (unpurified) 1/100 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tissue Plasminogen Activator antibody [EPR7232(2)] (ab157469)
Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling TPA Tissue Plasminogen Activator with ab157469 (unpurified) at 1/100 dilution.