AB157469

Anti-Tissue Plasminogen Activator antibody [EPR7232(2)]

Name: Anti-Tissue Plasminogen Activator antibody [EPR7232(2)] (ab157469) | Abcam
Brand: Abcam Limited
SKU: AB157469
Price: 360 USD
Availability: InStock
Rating: 3 (1 reviews)

BOND RX™ Validated
RabMAb
Recombinant
What is this?

(1 Review)

(13 Publications)

Rabbit Recombinant Monoclonal Tissue Plasminogen Activator antibody. Suitable for WB, IHC-P and reacts with Mouse, Rat, Human samples. Cited in 13 publications.

View Alternative Names

Tissue-type plasminogen activator, t-PA, t-plasminogen activator, tPA, PLAT

11 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tissue Plasminogen Activator antibody [EPR7232(2)] (AB157469)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tissue Plasminogen Activator antibody [EPR7232(2)] (AB157469)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human lung carcinoma tissue sections labeling TPA Tissue Plasminogen Activator with purified ab157469 at 1/1000 dilution (1.91 μg/ml). Heat mediated antigen retrieval was performed using Bond™ Epitope Retrieval Solution 2 (pH 9.0). Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control : PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tissue Plasminogen Activator antibody [EPR7232(2)] (AB157469)

ab157469 (unpurified) showing +ve staining in Human skeletal muscle.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tissue Plasminogen Activator antibody [EPR7232(2)] (AB157469)

ab157469 (unpurified) showing +ve staining in Human normal liver.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tissue Plasminogen Activator antibody [EPR7232(2)] (AB157469)

ab157469 (unpurified) showing +ve staining in Human normal kidney.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tissue Plasminogen Activator antibody [EPR7232(2)] (AB157469)

ab157469 (unpurified) showing +ve staining in Human normal brain.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tissue Plasminogen Activator antibody [EPR7232(2)] (AB157469)

Immunohistochemical analysis of paraffin-embedded Human cervial carcinoma tissue labeling TPA Tissue Plasminogen Activator with ab157469 (unpurified) 1/100 dilution.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tissue Plasminogen Activator antibody [EPR7232(2)] (AB157469)

Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling TPA Tissue Plasminogen Activator with ab157469 (unpurified) at 1/100 dilution.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tissue Plasminogen Activator antibody [EPR7232(2)] (AB157469)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat liver tissue sections labeling TPA Tissue Plasminogen Activator with purified ab157469 at 1/1000 dilution (1.91 μg/ml). Heat mediated antigen retrieval was performed using Bond™ Epitope Retrieval Solution 2 (pH 9.0). Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control : PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tissue Plasminogen Activator antibody [EPR7232(2)] (AB157469)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse liver tissue sections labeling TPA Tissue Plasminogen Activator with purified ab157469 at 1/1000 dilution (1.91 μg/ml). Heat mediated antigen retrieval was performed using Bond™ Epitope Retrieval Solution 2 (pH 9.0). Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control : PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

Lab

Western blot - Anti-Tissue Plasminogen Activator antibody [EPR7232(2)] (AB157469)

T-PA is serected and mainly released into blood and extracellular fluid to activate plasminogen. The molecular weight observed is consistent with what has been described in PMID : 22069469

All lanes:

Western blot - Anti-Tissue Plasminogen Activator antibody [EPR7232(2)] (ab157469) at 1/1000 dilution

Lane 1:

Mouse plasma lysates at 15 µg

Lane 2:

Rat plasma lysates at 15 µg

Lane 3:

Human plasma lysates at 15 µg

Lane 4:

Human brain lysates at 15 µg

Lane 5:

Human heart lysates at 15 µg

Lane 6:

Human liver lysates at 15 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 63 kDa

Observed band size: 68 kDa

false

Unknown

Western blot - Anti-Tissue Plasminogen Activator antibody [EPR7232(2)] (AB157469)

All lanes:

Western blot - Anti-Tissue Plasminogen Activator antibody [EPR7232(2)] (ab157469) at 1/1000 dilution

All lanes:

Human plasma lysate at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 63 kDa

false

Carrier free

Anti-Tissue Plasminogen Activator antibody [EPR7232(2)] - BSA and Azide free
HRP

HRP Anti-Tissue Plasminogen Activator antibody [EPR7232(2)]
Biotin

Biotin Anti-Tissue Plasminogen Activator antibody [EPR7232(2)]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR7232(2)

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

WB, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style

left-column

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "Perform heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0)." }, "Mouse": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "Perform heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0)." }, "Rat": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "Perform heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0)." } } }

style

left-column

Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

style

left-column

Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Tissue Plasminogen Activator (tPA) also known as a plasminogen activator acts mechanically to convert plasminogen to plasmin a serine protease. This reaction occurs at the surface of a fibrin clot leading to clot degradation a process known as fibrinolysis. tPA has a molecular mass of approximately 70 kDa. It is mainly expressed in vascular endothelial cells and is released into the bloodstream in response to stimuli such as circulatory stasis or endothelial damage.

Biological function summary

TPA plays a critical role in thrombolysis by breaking down blood clots into their soluble components. It regulates plasminogen function by cleaving this zymogen to yield the active protease plasmin. This function makes tPA integral in maintaining hemostasis and it does not form a part of a larger protein complex. The activity and the regulation of tPA are important for preventing pathologic clotting which can lead to cardiovascular complications.

Pathways

TPA is central to the fibrinolytic pathway. This pathway facilitates the conversion of plasminogen to plasmin enabling clot resolution. In addition tPA interacts with other proteins such as urokinase plasminogen activator (uPA) and inhibitors like plasminogen activator inhibitor-1 (PAI-1). The balance between tPA and its inhibitors is important for the regulation of fibrinolytic activity impacting hemostatic and thrombotic events.

TPA connects closely with conditions like stroke and myocardial infarction due to its thrombolytic properties. In ischemic stroke excessive or insufficient tPA activity can disrupt normal blood flow leading to tissue damage. Additionally in myocardial infarction tPA's role in breaking down clots proves important for restoring coronary blood flow. It is also linked with proteins like fibrinogen as they serve as substrates in the clot degradation process and with PAI-1 which modulates its activity and influences disease progression.

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

Converts the abundant, but inactive, zymogen plasminogen to plasmin by hydrolyzing a single Arg-Val bond in plasminogen. By controlling plasmin-mediated proteolysis, it plays an important role in tissue remodeling and degradation, in cell migration and many other physiopathological events. During oocyte activation, plays a role in cortical granule reaction in the zona reaction, which contributes to the block to polyspermy (By similarity).

See full target information PLAT

style

left-column

Publications (13)

Recent publications for all applications. Explore the full list and refine your search

CNS neuroscience & therapeutics 31:e70581 PubMed40994138

2025

Elucidating the Mechanism of Emodin in Treating Post-Stroke Depression Through Network Pharmacology and Animal Experiments.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaoju Liu,Jie Gao,Kai Yang,Weiming Zhu,Ming Su,Zichun Liu,Yaxin Yuan,Linya Cao,Tong Wu,Wei Liu

Discover oncology 15:582 PubMed39441449

2024

Overexpression of a disintegrin and metalloproteinase 9 (ADAM9) in relation to poor prognosis of patients with oral squamous cell carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Shuangjiang Wu,Lang Cheng,Tao Luo,Anupong Makeudom,Lei Wang,Suttichai Krisanaprakornkit

Translational cancer research 13:4866-4877 PubMed39430838

2024

RNA sequencing reveals transcriptomic changes in PC-12 cells following plasminogen activator, tissue type overexpression.

Applications

Unspecified application

Species

Unspecified reactive species

Yongxin Mao,Chen Fang,Juping Zhao,Xin Huang,Wei He,Chenghe Wang,Fukang Sun,Jun Dai

BMC pulmonary medicine 22:126 PubMed35379218

2022

Paradoxical development of pleural-based masses in patients with pleural tuberculosis during treatment: a clinical observational study in China.

Applications

Unspecified application

Species

Unspecified reactive species

Zhengwei Dong,Wei Zhang,Wenwen Sun,Shaojun Zhang,Chenlu Yang,Chunyan Wu,Lin Fan

Cancers 14: PubMed35053621

2022

Molecular Landscape of the Coagulome of Oral Squamous Cell Carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Marine Lottin,Simon Soudet,Julie Fercot,Floriane Racine,Julien Demagny,Jérémie Bettoni,Denis Chatelain,Marie-Antoinette Sevestre,Youcef Mammeri,Michele Lamuraglia,Antoine Galmiche,Zuzana Saidak

Reproductive sciences (Thousand Oaks, Calif.) 29:1001-1019 PubMed34796470

2021

The Plasminogen Activator System, Glucocorticoid, and Mineralocorticoid Receptors in the Primate Endometrium During Artificial Menstrual Cycles.

Applications

Unspecified application

Species

Unspecified reactive species

Esra Demirel,Reem Sabouni,Neelima Chandra,Ov D Slayden,David F Archer

Frontiers in cardiovascular medicine 7:584998 PubMed33173789

2020

Clinical Relevance of Plasma Endogenous Tissue-Plasminogen Activator and Aortic Valve Sclerosis: Performance as a Diagnostic Biomarker.

Applications

Unspecified application

Species

Unspecified reactive species

Zhongli Chen,Ying Shen,Qiqi Xue,Bo Wen Lin,Xiao Yan He,Yi Bo Zhang,Ying Yang,Wei Feng Shen,Ye Hong Liu,Ke Yang

Aging 12:17976-17989 PubMed32960786

2020

Prothrombotic effects of high uric acid in mice activation of MEF2C-dependent NF-κB pathway by upregulating let-7c.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaoyu Cheng,Tian Liu,Lidan Ma,Zhen Liu,Ying Xin,Zhaotong Jia,Ying Chen,Changgui Li,Ruixia Sun

Annals of palliative medicine 9:1965-1975 PubMed32692217

2020

Andrographolide protects against endothelial dysfunction and inflammatory response in rats with coronary heart disease by regulating PPAR and NF-κB signaling pathways.

Applications

Unspecified application

Species

Unspecified reactive species

Jia Shu,Ruizhen Huang,Ying Tian,Yanru Liu,Rui Zhu,Gang Shi

Journal of cellular physiology 234:22057-22070 PubMed31106414

2019

From clinic to mechanism: Proteomics-based assessment of angiogenesis in adrenal pheochromocytoma.

Applications

Unspecified application

Species

Unspecified reactive species

Fukang Sun,Ran Zhuo,Wenming Ma,Da Yang,Tingwei Su,Lei Ye,Danfeng Xu,Weiqing Wang

View all publications

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Tissue Plasminogen Activator antibody [EPR7232(2)]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tissue Plasminogen Activator antibody [EPR7232(2)] (AB157469)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tissue Plasminogen Activator antibody [EPR7232(2)] (AB157469)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tissue Plasminogen Activator antibody [EPR7232(2)] (AB157469)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tissue Plasminogen Activator antibody [EPR7232(2)] (AB157469)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tissue Plasminogen Activator antibody [EPR7232(2)] (AB157469)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tissue Plasminogen Activator antibody [EPR7232(2)] (AB157469)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tissue Plasminogen Activator antibody [EPR7232(2)] (AB157469)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tissue Plasminogen Activator antibody [EPR7232(2)] (AB157469)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tissue Plasminogen Activator antibody [EPR7232(2)] (AB157469)

Western blot - Anti-Tissue Plasminogen Activator antibody [EPR7232(2)] (AB157469)

Western blot - Anti-Tissue Plasminogen Activator antibody [EPR7232(2)] (AB157469)

Related conjugates and formulations (3)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Complementary Products

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (13)

Product promise