AB240146

Anti-Tissue Plasminogen Activator antibody [EPR7232(2)] - BSA and Azide free

BOND RX™ Validated
RabMAb
Recombinant
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(1 Publication)

Rabbit Recombinant Monoclonal Tissue Plasminogen Activator antibody. Carrier free. Suitable for WB, IHC-P and reacts with Mouse, Rat, Human samples. Cited in 1 publication.

View Alternative Names

Tissue-type plasminogen activator, t-PA, t-plasminogen activator, tPA, PLAT

9 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tissue Plasminogen Activator antibody [EPR7232(2)] - BSA and Azide free (AB240146)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tissue Plasminogen Activator antibody [EPR7232(2)] - BSA and Azide free (AB240146)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human lung carcinoma tissue sections labeling TPA Tissue Plasminogen Activator with purified ab157469 at 1/1000 dilution (1.91 μg/ml). Heat mediated antigen retrieval was performed using Bond™ Epitope Retrieval Solution 2 (pH 9.0). Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control : PBS instead of the primary antibody. Hematoxylin was used as a counterstain. This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab157469)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tissue Plasminogen Activator antibody [EPR7232(2)] - BSA and Azide free (AB240146)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tissue Plasminogen Activator antibody [EPR7232(2)] - BSA and Azide free (AB240146)

ab157469 (unpurified) showing +ve staining in Human skeletal muscle.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab157469).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tissue Plasminogen Activator antibody [EPR7232(2)] - BSA and Azide free (AB240146)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tissue Plasminogen Activator antibody [EPR7232(2)] - BSA and Azide free (AB240146)

ab157469 (unpurified) showing +ve staining in Human normal liver.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab157469).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tissue Plasminogen Activator antibody [EPR7232(2)] - BSA and Azide free (AB240146)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tissue Plasminogen Activator antibody [EPR7232(2)] - BSA and Azide free (AB240146)

ab157469 (unpurified) showing +ve staining in Human normal kidney.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab157469).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tissue Plasminogen Activator antibody [EPR7232(2)] - BSA and Azide free (AB240146)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tissue Plasminogen Activator antibody [EPR7232(2)] - BSA and Azide free (AB240146)

ab157469 (unpurified) showing +ve staining in Human normal brain.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab157469).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tissue Plasminogen Activator antibody [EPR7232(2)] - BSA and Azide free (AB240146)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tissue Plasminogen Activator antibody [EPR7232(2)] - BSA and Azide free (AB240146)

Immunohistochemical analysis of paraffin-embedded Human cervial carcinoma tissue labeling TPA Tissue Plasminogen Activator with ab157469 (unpurified) 1/100 dilution.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab157469).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tissue Plasminogen Activator antibody [EPR7232(2)] - BSA and Azide free (AB240146)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tissue Plasminogen Activator antibody [EPR7232(2)] - BSA and Azide free (AB240146)

Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling TPA Tissue Plasminogen Activator with ab157469 (unpurified) at 1/100 dilution.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab157469).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tissue Plasminogen Activator antibody [EPR7232(2)] - BSA and Azide free (AB240146)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tissue Plasminogen Activator antibody [EPR7232(2)] - BSA and Azide free (AB240146)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat liver tissue sections labeling TPA Tissue Plasminogen Activator with purified ab157469 at 1/1000 dilution (1.91 μg/ml). Heat mediated antigen retrieval was performed using Bond™ Epitope Retrieval Solution 2 (pH 9.0). Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control : PBS instead of the primary antibody. Hematoxylin was used as a counterstain. This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab157469)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tissue Plasminogen Activator antibody [EPR7232(2)] - BSA and Azide free (AB240146)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tissue Plasminogen Activator antibody [EPR7232(2)] - BSA and Azide free (AB240146)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse liver tissue sections labeling TPA Tissue Plasminogen Activator with purified ab157469 at 1/1000 dilution (1.91 μg/ml). Heat mediated antigen retrieval was performed using Bond™ Epitope Retrieval Solution 2 (pH 9.0). Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control : PBS instead of the primary antibody. Hematoxylin was used as a counterstain. This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab157469)

Unconjugated

Anti-Tissue Plasminogen Activator antibody [EPR7232(2)]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR7232(2)

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse, Rat, Human

Applications

IHC-P, WB

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p>Perform heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0).</p>" }, "Mouse": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Product details

ab240146 is the carrier-free version of ab157469.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Tissue Plasminogen Activator (tPA) also known as a plasminogen activator acts mechanically to convert plasminogen to plasmin a serine protease. This reaction occurs at the surface of a fibrin clot leading to clot degradation a process known as fibrinolysis. tPA has a molecular mass of approximately 70 kDa. It is mainly expressed in vascular endothelial cells and is released into the bloodstream in response to stimuli such as circulatory stasis or endothelial damage.

Biological function summary

TPA plays a critical role in thrombolysis by breaking down blood clots into their soluble components. It regulates plasminogen function by cleaving this zymogen to yield the active protease plasmin. This function makes tPA integral in maintaining hemostasis and it does not form a part of a larger protein complex. The activity and the regulation of tPA are important for preventing pathologic clotting which can lead to cardiovascular complications.

Pathways

TPA is central to the fibrinolytic pathway. This pathway facilitates the conversion of plasminogen to plasmin enabling clot resolution. In addition tPA interacts with other proteins such as urokinase plasminogen activator (uPA) and inhibitors like plasminogen activator inhibitor-1 (PAI-1). The balance between tPA and its inhibitors is important for the regulation of fibrinolytic activity impacting hemostatic and thrombotic events.

TPA connects closely with conditions like stroke and myocardial infarction due to its thrombolytic properties. In ischemic stroke excessive or insufficient tPA activity can disrupt normal blood flow leading to tissue damage. Additionally in myocardial infarction tPA's role in breaking down clots proves important for restoring coronary blood flow. It is also linked with proteins like fibrinogen as they serve as substrates in the clot degradation process and with PAI-1 which modulates its activity and influences disease progression.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Converts the abundant, but inactive, zymogen plasminogen to plasmin by hydrolyzing a single Arg-Val bond in plasminogen. By controlling plasmin-mediated proteolysis, it plays an important role in tissue remodeling and degradation, in cell migration and many other physiopathological events. During oocyte activation, plays a role in cortical granule reaction in the zona reaction, which contributes to the block to polyspermy (By similarity).

See full target information PLAT

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Bio-protocol 15:e5424 PubMed40873476

2025

Assessing Human Treg Suppression at Single-Cell Resolution Using Mass Cytometry.

Applications

Unspecified application

Species

Unspecified reactive species

Jonas Nørskov Søndergaard,Janyerkye Tulyeu,David Priest,Shimon Sakaguchi,James B Wing

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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