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AB203623

Anti-Titin antibody

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(1 Publication)

Rabbit Polyclonal TITIN antibody. Suitable for IHC-P and reacts with Rat samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human TTN aa 2650 to C-terminus conjugated to Keyhole Limpet Haemocyanin.

View Alternative Names

Titin, Connectin, Rhabdomyosarcoma antigen MU-RMS-40.14, TTN

1 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Titin antibody (AB203623)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Titin antibody (AB203623)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded rat heart tissue labeling Titin with ab203623 at 1/200 dilution, followed by conjugation to the secondary antibody and DAB staining.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Rat

Applications

IHC-P

applications

Immunogen

Synthetic Peptide within Human TTN aa 2650 to C-terminus conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information.

Q8WZ42

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/500", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol." } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Preservative: 0.02% Proclin 300 Constituents: 50% Glycerol (glycerin, glycerine), 48.98% TBS, 1X, 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Titin also known by its full name connectin is a giant protein that plays an essential mechanical role in muscle contraction. It is the largest known protein with a molecular mass of approximately 3800 kDa. Titin is expressed mainly in cardiac and skeletal muscle tissues where it functions as a molecular spring. It provides structural support and stability to the sarcomere which is the basic contractile unit of muscle tissues.
Biological function summary

This protein titin contributes significantly to the elasticity and extensibility of the muscle. Titin is an integral part of the thick filament in muscle fibers and interacts directly with actin and myosin linking them together. It belongs to the muscle protein complex forming the backbone around which other proteins assemble. Titin allows muscles to maintain passive tension and recoil after stretching contributing to muscle elasticity during contraction.

Pathways

Several important functions depend on the intricate arrangement of titin within the sarcomere structure. Titin is involved in the regulatory pathways of muscle assembly and integrity. An important pathway includes the mechanical signaling pathway where titin acts alongside proteins like myosin-binding protein C. Titin also contributes to muscle protein turnover by interacting with ubiquitin-proteasome pathway components though it primarily serves as a scaffold for other molecules within these complex pathways.

Mutations in the titin gene associate with a range of debilitating conditions especially cardiomyopathy and muscular dystrophy. In particular dilated cardiomyopathy often results from titin mutations affecting the heart muscle's function. Proteins like myosin can become dysregulated when titin function is compromised further contributing to the disorder's pathology. Investigating these connections helps reveal how alterations in titin impact muscle-related diseases.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Key component in the assembly and functioning of vertebrate striated muscles. By providing connections at the level of individual microfilaments, it contributes to the fine balance of forces between the two halves of the sarcomere. The size and extensibility of the cross-links are the main determinants of sarcomere extensibility properties of muscle. In non-muscle cells, seems to play a role in chromosome condensation and chromosome segregation during mitosis. Might link the lamina network to chromatin or nuclear actin, or both during interphase.
See full target information TTN
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Critical reviews in eukaryotic gene expression 32:11-20 PubMed35997114

2022

LncRNA PCED1B-AS1 Upregulation in Hepatocellular Carcinoma and Regulation of the miR-10a/BCL6 Axis to Promote Cell Proliferation.

Applications

Unspecified application

Species

Unspecified reactive species

Haitao Ding,Juan He,Weili Xiao,Zhihong Ren,Wanting Gao
View all publications
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Product promise

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