Anti-Titin antibody [EPR26167-75] 20 ul size (ab307446)

Rabbit Recombinant Monoclonal TITIN antibody. Suitable for mIHC, ICC/IF, IHC-Fr, IHC-P and reacts with Mouse, Human, Rat samples.

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Images

Multiplex immunohistochemistry - Anti-Titin antibody [EPR26167-75] (AB307446), expandable thumbnail

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Form: Liquid
Clonality: Monoclonal

Reactivity data

Select an application

Product promiseTestedExpectedPredictedNot recommended

	mIHC	IP	ICC/IF	IHC-Fr	IHC-P	WB
Human	Tested	Not recommended	Expected	Expected	Tested	Not recommended
Mouse	Tested	Not recommended	Tested	Tested	Tested	Not recommended
Rat	Expected	Not recommended	Expected	Tested	Tested	Not recommended

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info 1/1000	Notes -
Species Human	Dilution info 1/1000	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Expected
Expected

Species	Dilution info	Notes
Species Rat	Dilution info Use at an assay dependent concentration.	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Rat, Mouse, Human	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info 1/500	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Human, Rat	Dilution info Use at an assay dependent concentration.	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Rat	Dilution info 1/100	Notes -
Species Mouse	Dilution info 1/100	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Human	Dilution info 1/100	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Rat	Dilution info 1/1000	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Species Mouse	Dilution info 1/1000	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Species Human	Dilution info 1/1000	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species	Dilution info	Notes
Species Rat, Mouse, Human	Dilution info -	Notes -

Target data

See full target information TTN

Function

Key component in the assembly and functioning of vertebrate striated muscles. By providing connections at the level of individual microfilaments, it contributes to the fine balance of forces between the two halves of the sarcomere. The size and extensibility of the cross-links are the main determinants of sarcomere extensibility properties of muscle. In non-muscle cells, seems to play a role in chromosome condensation and chromosome segregation during mitosis. Might link the lamina network to chromatin or nuclear actin, or both during interphase.

Alternative names

Titin, Connectin, Rhabdomyosarcoma antigen MU-RMS-40.14, TTN

Recommended products

Rabbit Recombinant Monoclonal TITIN antibody. Suitable for mIHC, ICC/IF, IHC-Fr, IHC-P and reacts with Mouse, Human, Rat samples.

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Form: Liquid
Clonality: Monoclonal
Clone number: EPR26167-75
Purification technique: Affinity purification Protein A
Concentration

Storage

Shipped at conditions: Blue Ice
Appropriate short-term storage duration: 1-2 weeks
Appropriate short-term storage conditions: +4°C
Appropriate long-term storage conditions: -20°C
Aliquoting information: Upon delivery aliquot
Storage information: Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

Titin also known by its full name connectin is a giant protein that plays an essential mechanical role in muscle contraction. It is the largest known protein with a molecular mass of approximately 3800 kDa. Titin is expressed mainly in cardiac and skeletal muscle tissues where it functions as a molecular spring. It provides structural support and stability to the sarcomere which is the basic contractile unit of muscle tissues.

Biological function summary

This protein titin contributes significantly to the elasticity and extensibility of the muscle. Titin is an integral part of the thick filament in muscle fibers and interacts directly with actin and myosin linking them together. It belongs to the muscle protein complex forming the backbone around which other proteins assemble. Titin allows muscles to maintain passive tension and recoil after stretching contributing to muscle elasticity during contraction.

Pathways

Several important functions depend on the intricate arrangement of titin within the sarcomere structure. Titin is involved in the regulatory pathways of muscle assembly and integrity. An important pathway includes the mechanical signaling pathway where titin acts alongside proteins like myosin-binding protein C. Titin also contributes to muscle protein turnover by interacting with ubiquitin-proteasome pathway components though it primarily serves as a scaffold for other molecules within these complex pathways.

Associated diseases and disorders

Mutations in the titin gene associate with a range of debilitating conditions especially cardiomyopathy and muscular dystrophy. In particular dilated cardiomyopathy often results from titin mutations affecting the heart muscle's function. Proteins like myosin can become dysregulated when titin function is compromised further contributing to the disorder's pathology. Investigating these connections helps reveal how alterations in titin impact muscle-related diseases.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
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13 product images

Multiplex immunohistochemistry - Anti-Titin antibody [EPR26167-75] (ab307446)
Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded Mouse cardiac muscle tissue staining Titin with ab307446 at a 1/1000 ( 0.475 µg/ml) dilution, CTNNA3 with Anti-CTNNA3 antibody [EPR18307] ab184916 at 1/2000 ( 0.942 µg/ml) dilution and Dystrophin with Anti-Dystrophin antibody [EPR21189] ab218198 at 1/5000 ( 0.110 µg/ml) dilution.
Panel A: merged staining of anti-Titin (gray; Opal™570), anti-CTNNA3 (green; Opal™520) and anti-Dystrophin (magenta; Opal™690) on mouse cardiac muscle.
Panel B: anti-Titin staining nucleus and cytoplasm in mouse cardiac muscle.
Panel C: anti-CTNNA3 staining intercalated discs in mouse cardiac muscle.
Panel D: anti-Dystrophin staining membrane in mouse cardiac muscle.
Nuclear DNA was labeled with DAPI (shown in blue).
The section was incubated in three rounds of staining: in the order of ab307446, Anti-CTNNA3 antibody [EPR18307] ab184916 and Anti-Dystrophin antibody [EPR21189] ab218198 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.
The immunostaining was performed on a Leica Biosystems BOND^° RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0 epitope retrieval solution2) for 20 mins.
Multiplex immunohistochemistry - Anti-Titin antibody [EPR26167-75] (ab307446)
Fluorescence multiplex immunohistochemical analysis of the human cardiac muscle (Formalin/PFA-fixed paraffin-embedded sections).

Panel A: merged staining of anti-delta Sarcoglycan (Anti-delta Sarcoglycan antibody [EPR8706] ab137101, red; Opal™690), anti-Titin (ab307446, green; Opal™520) and anti-Natriuretic peptides A (Anti-Natriuretic peptides A antibody [EPR20247] ab209232, magenta; Opal™570) on human cardiac muscle. Panel B: anti-Titin displayed nucleus and cytoplasm expression. Panel C: anti-Natriuretic peptides A displayed granular cytoplasmic expression. Panel D: anti-delta Sarcoglycan displayed membrane expression. Opal Polymer HRP Ms + Rb was used as a secondary antibody.

The section was incubated in three rounds of staining: in the order of Anti-delta Sarcoglycan antibody [EPR8706] ab137101 at 1/1000 (1.043 μg/ml) dilution, ab307446 at 1/500 (0.95 μg/ml) dilution, and Anti-Natriuretic peptides A antibody [EPR20247] ab209232 at 1/3000 (0.241 μg/ml) dilution for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system. DAPI (blue) was used as a nuclear counter stain.

The immunostaining was performed on a Leica Biosystems BOND^® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Titin antibody [EPR26167-75] (ab307446)
Immunohistochemical analysis of paraffin-embedded Rat liver tissue lABeling Titin with ab307446 at 1/1000 (0.475 ug/ml) followed by a ready to use Goat Anti-RABbit IgG H&L (HRP polymer). Negative control: No staining on rat liver.The section was incubated with ab307446 at 4ЎгC overnight. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-RABbit IgG H&L (HRP polymer).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Titin antibody [EPR26167-75] (ab307446)
Immunohistochemical analysis of paraffin-embedded Rat cardiac muscle tissue lABeling Titin with ab307446 at 1/1000 (0.475 ug/ml) followed by a ready to use Goat Anti-RABbit IgG H&L (HRP polymer). Positive staining on rat cardiac muscle.The section was incubated with ab307446 at 4ЎгC overnight. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-RABbit IgG H&L (HRP polymer).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Titin antibody [EPR26167-75] (ab307446)
Immunohistochemical analysis of paraffin-embedded Mouse liver tissue lABeling Titin with ab307446 at 1/1000 (0.475 ug/ml) followed by a ready to use Goat Anti-RABbit IgG H&L (HRP polymer). Negative control: No staining on mouse liver (PMID: 15752755).The section was incubated with ab307446 at 4ЎгC overnight. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-RABbit IgG H&L (HRP polymer).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Titin antibody [EPR26167-75] (ab307446)
Immunohistochemical analysis of paraffin-embedded Mouse cardiac muscle tissue lABeling Titin with ab307446 at 1/1000 (0.475 ug/ml) followed by a ready to use Goat Anti-RABbit IgG H&L (HRP polymer). Positive staining on mouse cardiac muscle (PMID: 31757849).The section was incubated with ab307446 at 4ЎгC overnight. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-RABbit IgG H&L (HRP polymer).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Titin antibody [EPR26167-75] (ab307446)
Immunohistochemical analysis of paraffin-embedded Human liver tissue lABeling Titin with ab307446 at 1/1000 (0.475 ug/ml) followed by a ready to use Goat Anti-RABbit IgG H&L (HRP polymer). Negative control: No staining on human liver.The section was incubated with ab307446 at 4ЎгC overnight. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-RABbit IgG H&L (HRP polymer).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Titin antibody [EPR26167-75] (ab307446)
Immunohistochemical analysis of paraffin-embedded Human cardiac muscle tissue lABeling Titin with ab307446 at 1/1000 (0.475 ug/ml) followed by a ready to use Goat Anti-RABbit IgG H&L (HRP polymer). Positive staining on human cardiac muscle.The section was incubated with ab307446 at 4ЎгC overnight. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-RABbit IgG H&L (HRP polymer).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
Immunohistochemistry (Frozen sections) - Anti-Titin antibody [EPR26167-75] (ab307446)
Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen rat liver (fresh) tissue labeling Titin with ab307446 at 1/100 dilution (4.75 µg/ml) followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution (2 µg/mL) (Green).
Negative control: confocal image showing no staining on rat liver (PMID: 15752755).
The nuclear counterstain was DAPI (Blue). The section was incubated with ab307446 for 60 mins at room temperature. The section was then mounted using Fluoromount®. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Secondary antibody control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution (2 µg/mL).
Immunohistochemistry (Frozen sections) - Anti-Titin antibody [EPR26167-75] (ab307446)
Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen rat cardiac muscle (fresh) tissue labeling Titin with ab307446 at 1/100 dilution (4.75 µg/ml) followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution (2 µg/mL) (Green).
Confocal image showing positive staining on rat cardiac muscle.
The nuclear counterstain was DAPI (Blue). The section was incubated with ab307446 for 60 mins at room temperature. The section was then mounted using Fluoromount®. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Secondary antibody control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution (2 µg/mL).
Immunohistochemistry (Frozen sections) - Anti-Titin antibody [EPR26167-75] (ab307446)
Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeABilized frozen Mouse liver (fresh) tissue lABeling Titin with ab307446 at 1/100 (4.75 ug/ml) dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-RABbit IgG H&L (Alexa Fluor? 488) preadsorbed at 1/1000 2 ug/mL dilution (Green). Negative control: confocal image showing no staining on mouse liver (PMID: 15752755). The nuclear counterstain was DAPI (Blue). The section was incubated with ab307446 for 60 mins at room temperature. The section was then mounted using Fluoromount?.The immunostaining was performed on a Leica Biosystems BOND? RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). is observed. The nuclear counterstain was DAPI (Blue). Secondary antibody control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-RABbit IgG H&L (Alexa Fluor? 488) preadsorbedat 1/1000 2 ug/mL dilution.
Immunohistochemistry (Frozen sections) - Anti-Titin antibody [EPR26167-75] (ab307446)
Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeABilized frozen Mouse cardiac muscle (fresh) tissue lABeling Titin with ab307446 at 1/100 (4.75 ug/ml) dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-RABbit IgG H&L (Alexa Fluor? 488) preadsorbed at 1/1000 2 ug/mL dilution (Green). Confocal image showing positive staining on mouse cardiac muscle. The nuclear counterstain was DAPI (Blue). The section was incubated with ab307446 for 60 mins at room temperature. The section was then mounted using Fluoromount?.The immunostaining was performed on a Leica Biosystems BOND? RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). is observed. The nuclear counterstain was DAPI (Blue). Secondary antibody control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-RABbit IgG H&L (Alexa Fluor? 488) preadsorbedat 1/1000 2 ug/mL dilution.
Immunocytochemistry/ Immunofluorescence - Anti-Titin antibody [EPR26167-75] (ab307446)
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeABilized C2C12 (mouse myoblast) cells lABelling Titin with ab307446 at 1/500 (0.95 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-RABbit IgG H&L (Alexa Fluor? 488) preadsorbed antibody at 1/1000 2 ug/ml dilution (Green). Confocal image showing cytoplasmic and membranous staining in differentiated C2C12 cells and no staining in undifferentiated C2C12 cells.Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8). is observed. Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor? 594) was used to counterstain tubulin at 1/200 2.5 ug/ml dilution (Red). The Nuclear counterstain was DAPI (Blue). Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-RABbit IgG H&L (Alexa Fluor? 488) preadsorbed at 1/1000 2 ug/ml dilution.