AB224314

Anti-TJP2/ZO2 antibody

Name: Anti-TJP2/ZO2 antibody (ab224314) | Abcam
Brand: Abcam Limited
SKU: AB224314
Price: 630 USD
Availability: InStock

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(5 Publications)

Rabbit Polyclonal TJP2/ZO2 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 5 publications. Immunogen corresponding to Recombinant Fragment Protein within Human TJP2 aa 300-500.

View Alternative Names

X104, ZO2, TJP2, Tight junction protein 2, Tight junction protein ZO-2, Zona occludens protein 2, Zonula occludens protein 2

6 Images

Immunocytochemistry/ Immunofluorescence - Anti-TJP2/ZO2 antibody (AB224314)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-TJP2/ZO2 antibody (AB224314)

PFA-fixed/Triton X-100 permeabilized U-2 OS (human bone osteosarcoma epithelial cell line) cells stained for TJP2/ZO2 (green) using ab224314 (4 μg/ml) in ICC/IF.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TJP2/ZO2 antibody (AB224314)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TJP2/ZO2 antibody (AB224314)

Paraffin-embedded human pancreas stained for TJP2 using ab224314 at 1/50 dilution in immunohistochemical analysis. Shows weak cytoplasmic positivity in exocrine glandular cells.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TJP2/ZO2 antibody (AB224314)

Paraffin-embedded human cerebral cortex stained for TJP2 using ab224314 at 1/50 dilution in immunohistochemical analysis. Shows strong cytoplasmic positivity in glial cells.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TJP2/ZO2 antibody (AB224314)

Paraffin-embedded human testis stained for TJP2 using ab224314 at 1/50 dilution in immunohistochemical analysis. Shows strong cytoplasmic and membranous positivity in cells in seminiferous ducts.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TJP2/ZO2 antibody (AB224314)

Paraffin-embedded human colon stained for TJP2 using ab224314 at 1/50 dilution in immunohistochemical analysis. Shows moderate cytoplasmic and membranous positivity in glandular cells.

Unknown

Western blot - Anti-TJP2/ZO2 antibody (AB224314)

Loading control : Anti-HSP90B1

All lanes:

ab224314 at 0.4 µg/mL

Lane 1:

Human A-431 cell lines

Lane 2:

Human SK-MEL-30 cell line

Predicted band size: 134 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

ICC/IF, IHC-P, WB

applications

Immunogen

Recombinant Fragment Protein within Human TJP2 aa 300-500. The exact immunogen used to generate this antibody is proprietary information.

Q9UDY2

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/200", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "Fixation/Permeabilization: PFA/Triton X-100." }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Dog": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

TJP2 also known as ZO-2 is a tight junction protein with an approximate molecular mass of 160 kDa. It belongs to the membrane-associated guanylate kinase homologue (MAGUK) family and is expressed in various epithelial and endothelial tissues. ZO-2 is located at the tight junctions where it interacts with other proteins to maintain cell-cell adhesion and contribute to the integrity of the cellular barrier. This protein contains multiple domains that facilitate interactions with membrane proteins actin cytoskeleton and nuclear components highlighting its multifunctional nature.

Biological function summary

TJP2/ZO-2 modulates the permeability and selectivity of the paracellular pathway by being a part of the tight junction complex. It acts as a scaffold protein connecting transmembrane proteins such as occludin and claudins with cytoplasmic signaling molecules. Through its interaction with the actin cytoskeleton ZO-2 maintains the structure and function of the tight junctions playing a critical role in establishing and regulating epithelial barrier function. Additionally it participates in the regulation of gene expression by shuttling between the cytoplasm and the nucleus.

Pathways

The ZO-2 protein is involved in both the epithelial transport and tight junction signaling pathways. Within the epithelial transport pathway ZO-2 coordinates with other tight junction components like ZO-1 to control the movement of ions and molecules. In tight junction signaling ZO-2 plays a role with proteins such as FAK and SRC in relaying signals that influence cell proliferation and differentiation. It connects to the Hippo pathway which regulates growth and apoptosis by interacting with YAP and TAZ proteins thereby influencing cellular homeostasis and tissue organization.

Mutations or altered expression of TJP2/ZO-2 are associated with liver-related conditions such as cholestasis and hypercholanemia. These conditions highlight the protein’s role in maintaining bile duct integrity and function. Moreover ZO-2 dysregulation can involve disorders related to epithelial barrier function like inflammatory bowel disease. There is an established connection to proteins like occludin and claudin which when impacted mirrors ZO-2's deficiencies pointing towards its involvement in proper epithelial barrier maintenance and response to pathophysiological changes.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Plays a role in tight junctions and adherens junctions (By similarity). Acts as a positive regulator of RANKL-induced osteoclast differentiation, potentially via mediating downstream transcriptional activity (By similarity).

See full target information TJP2

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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

Scientific reports 14:12314 PubMed38811617

2024

A novel strategy to facilitate uniform epithelial cell maturation using liquid-liquid interfaces.

Applications

Unspecified application

Species

Unspecified reactive species

Rie Sonoi,Masamichi Kamihira

Journal of gastroenterology and hepatology 38:1158-1169 PubMed36878870

2023

Lactobacillus rhamnosus GG alleviates colitis caused by chemotherapy via biofilm formation.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaolan Qiu,Xuedong Han,Xiangcheng Zhang,Lucy A Teng,Mukesh K Sriwastva,Linlin Zhen,Zhi Li,Minmin Liu,Yi Ren,Shui Wang

Neural regeneration research 17:812-818 PubMed34472480

2021

CircLphn3 protects the blood-brain barrier in traumatic brain injury.

Applications

Unspecified application

Species

Unspecified reactive species

Yu-Qi Cheng,Chen-Rui Wu,Meng-Ran Du,Qiang Zhou,Bi-Ying Wu,Jia-Yuan-Yuan Fu,Ehab Balawi,Wei-Lin Tan,Zheng-Bu Liao

Gastroenterology 159:1471-1486.e12 PubMed32553762

2020

Human Pluripotent Stem Cell-Derived Organoids as Models of Liver Disease.

Applications

Unspecified application

Species

Unspecified reactive species

Muhammad Nadzim Bin Ramli,Yee Siang Lim,Chwee Tat Koe,Deniz Demircioglu,Weiquan Tng,Kevin Andrew Uy Gonzales,Cheng Peow Tan,Iwona Szczerbinska,Hongqing Liang,Einsi Lynn Soe,Zhiping Lu,Chaiyaboot Ariyachet,Ka Man Yu,Shu Hui Koh,Lai Ping Yaw,Nur Halisah Binte Jumat,John Soon Yew Lim,Graham Wright,Asim Shabbir,Yock Young Dan,Huck-Hui Ng,Yun-Shen Chan

Journal of cancer research and clinical oncology 146:2189-2203 PubMed32488496

2020

Prostaglandin E2 receptor 3 (EP3) signaling promotes migration of cervical cancer via urokinase-type plasminogen activator receptor (uPAR).

Applications

Unspecified application

Species

Unspecified reactive species

Yao Ye,Lin Peng,Aurelia Vattai,Eileen Deuster,Christina Kuhn,Christian Dannecker,Sven Mahner,Udo Jeschke,Viktoria von Schönfeldt,Helene H Heidegger

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-TJP2/ZO2 antibody

Immunocytochemistry/ Immunofluorescence - Anti-TJP2/ZO2 antibody (AB224314)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TJP2/ZO2 antibody (AB224314)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TJP2/ZO2 antibody (AB224314)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TJP2/ZO2 antibody (AB224314)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TJP2/ZO2 antibody (AB224314)

Western blot - Anti-TJP2/ZO2 antibody (AB224314)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

Primary Antibodies

Complementary Products

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (5)

Product promise