Anti-TJP2/ZO2 antibody - C-terminal

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TJP2/ZO2 antibody - C-terminal (AB191133)

TJP2 was detected in a paraffin-embedded section of mouse liver tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 µg/ml ab191133 overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.

• ICC

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Immunocytochemistry - Anti-TJP2/ZO2 antibody - C-terminal (AB191133)

TJP2 was detected in an immunocytochemical section of U2OS cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 µg/mL ab191133 overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1/500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TJP2/ZO2 antibody - C-terminal (AB191133)

TJP2 was detected in a paraffin-embedded section of rat kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 µg/ml ab191133 overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.

• WB

Supplier Data

Western blot - Anti-TJP2/ZO2 antibody - C-terminal (AB191133)

Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with ab191133 at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1/5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for TJP2 at approximately 150 kDa. The expected band size for TJP2 is at 131 kDa.

All lanes:

Western blot - Anti-TJP2/ZO2 antibody - C-terminal (ab191133) at 0.5 µg/mL

Lane 1:

human RT4 whole cell lysates, at 30 µg

Lane 2:

human A431 whole cell lysates, at 30 µg

Lane 3:

human HepG2 whole cell lysates, at 30 µg

Lane 4:

human MCF-7 whole cell lysates, at 30 µg

Lane 5:

human U2OS whole cell lysates, at 30 µg

Lane 6:

human Hela whole cell lysates, at 30 µg

Lane 7:

human PC-3 whole cell lysates, at 30 µg

Lane 8:

rat RH35 whole cell lysates, at 30 µg

Lane 9:

rat HEPA1-6 whole cell lysates. at 30 µg

Secondary

All lanes:

goat anti-rabbit IgG-HRP at 1/5000 dilution

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