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AB155662

Anti-TKTL1/TKR antibody

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(2 Publications)

Rabbit Polyclonal TKTL1/TKR antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human TKTL1 aa 1-350.

View Alternative Names

TKR, TKT2, TKTL1, Transketolase-like protein 1, Transketolase 2, Transketolase-related protein, TK 2

3 Images
Immunocytochemistry/ Immunofluorescence - Anti-TKTL1/TKR antibody (AB155662)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-TKTL1/TKR antibody (AB155662)

Immunofluorescence analysis of paraformaldehyde-fixed A431 cells, labeling TKTL1/TKR using ab155662 at a 1/200 dilution. The image in the lower panel was costained with Hoechst 33342.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TKTL1/TKR antibody (AB155662)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TKTL1/TKR antibody (AB155662)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human SAS xenograft tissue, labeling TKTL1/TKR using ab155662 at a 1/100 dilution.

Western blot - Anti-TKTL1/TKR antibody (AB155662)
  • WB

Unknown

Western blot - Anti-TKTL1/TKR antibody (AB155662)

7.5% SDS PAGE

All lanes:

Western blot - Anti-TKTL1/TKR antibody (ab155662) at 1/5000 dilution

Lane 1:

HeLa whole cell lysate at 30 µg

Lane 2:

HepG2 whole cell lysate at 30 µg

Lane 3:

MOLT4 whole cell lysate at 30 µg

Predicted band size: 65 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, IHC-P, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human TKTL1 aa 1-350. The exact immunogen used to generate this antibody is proprietary information.

P51854

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "<p>Antigen retrieval also possible using Tris-EDTA buffer (pH8.0).</p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 Preservative: 0.025% Proclin 300 Constituents: PBS, 20% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The TKTL1 protein also known as transketolase-like 1 or TKR is an important enzyme involved in cellular metabolism. It has a molecular mass of approximately 65 kDa. TKTL1 is widely expressed throughout various tissues with notably high levels of expression in the liver and brain. Mechanically it functions by participating in the non-oxidative branch of the pentose phosphate pathway facilitating the transfer of two-carbon units between sugar phosphates.
Biological function summary

TKTL1 supports numerous cellular processes by influencing the cell's redox state and nucleotide synthesis. Its activities help drive cell proliferation and survival often seen in rapidly dividing cells. Although not typically part of a macromolecular complex TKTL1 associates with other cellular components to execute its function. It plays a role in energy production and biosynthetic processes indirectly supporting the metabolic demands of cells.

Pathways

TKTL1 is involved in the pentose phosphate pathway and glycolysis. These pathways are critical for maintaining cellular homeostasis and generating the necessary biomolecules for cell growth. It operates alongside other proteins such as transketolase and transaldolase to ensure the proper flow of metabolites within these pathways. Additionally TKTL1's role affects the balance of nicotinamide adenine dinucleotide phosphate (NADPH) which is essential for reductive biosynthesis and antioxidant defense.

TKTL1 has associations with several cancers including colorectal and breast cancer where its expression levels can impact tumor growth and metastasis. The protein's connection to cancer is partly due to its influence on cell proliferation and metabolic reprogramming. Furthermore TKTL1's interaction with hypoxia-inducible factors (HIFs) contributes to its involvement in hypoxic tumor environments. Studying TKTL1 in these contexts helps to understand the metabolic shifts contributing to carcinogenesis.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Catalyzes the transfer of a two-carbon ketol group from a ketose donor to an aldose acceptor, via a covalent intermediate with the cofactor thiamine pyrophosphate.. Isoform 4. During fetal neocortex development, may be essential to maintain the full number of basal radial glia (bRG). bRG are neural progenitor cells that undergo asymmetric divisions, generating a bRG (self-renewal) and a neuron, in contrast to basal intermediate progenitors (bIPs), which typically divide once to give rise to 2 neurons. bRG generate more cortical neurons over time than bIPs.
See full target information TKTL1
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

American journal of physiology. Cell physiology 319:C910-C921 PubMed32903032

2020

Spatial locations of certain enzymes and transporters within preinvasive ductal epithelial cells predict human breast cancer recurrences.

Applications

Unspecified application

Species

Unspecified reactive species

Alexandra M Kraft,Howard R Petty

Frontiers in oncology 9:213 PubMed31001477

2019

Over Expressed TKTL1, CIP-2A, and B-MYB Proteins in Uterine Cervix Epithelium Scrapings as Potential Risk Predictive Biomarkers in HR-HPV-Infected LSIL/ASCUS Patients.

Applications

Unspecified application

Species

Unspecified reactive species

Anna Chiarini,Daisong Liu,Mario Rassu,Ubaldo Armato,Claudio Eccher,Ilaria Dal Prà
View all publications
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Product promise

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