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AB131331

Anti-TKTL2 antibody [EPR8592]

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(1 Publication)

Rabbit Recombinant Monoclonal TKTL2 antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 1 publication.

View Alternative Names

Transketolase-like protein 2, TKTL2

3 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TKTL2 antibody [EPR8592] (AB131331)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TKTL2 antibody [EPR8592] (AB131331)

Immunohistochemical analysis of paraffin-embedded Human hepatocellular carcinoma tissue labelling TKTL2 with ab131331 at 1/100 dilution.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Western blot - Anti-TKTL2 antibody [EPR8592] (AB131331)
  • WB

Unknown

Western blot - Anti-TKTL2 antibody [EPR8592] (AB131331)

All lanes:

Western blot - Anti-TKTL2 antibody [EPR8592] (ab131331) at 1/1000 dilution

Lane 1:

Jurkat cell lysate at 10 µg

Lane 2:

HepG2 cell lysate at 10 µg

Lane 3:

BxPC-3 cell lysate at 10 µg

Lane 4:

MCF7 cell lysate at 10 µg

Secondary

All lanes:

Goat anti-rabbit HRP at 1/2000 dilution

Predicted band size: 68 kDa

false

OI-RD Scanning - Anti-TKTL2 antibody [EPR8592] (AB131331)
  • OI-RD Scanning

Unknown

OI-RD Scanning - Anti-TKTL2 antibody [EPR8592] (AB131331)

We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody. Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.

  • Carrier free

    Anti-TKTL2 antibody [EPR8592] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR8592

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/250", "IHCP-species-notes": "<p></p>", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Storage information
Stable for 12 months at -20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Transketolase-like 2 (TKTL2) also known as TKL2 is an enzyme involved in carbohydrate metabolism. Its molecular weight is approximately 70 kDa. TKTL2 expression occurs broadly in human tissues with reported high expression levels in the heart brain and liver. The enzyme plays an important role in the non-oxidative part of the pentose phosphate pathway where it influences the interconversion of sugar phosphates.
Biological function summary

TKTL2 contributes to cellular processes related to energy metabolism and biosynthesis. It forms a functional relationship with other proteins in metabolic pathways participating in the regulation of cellular redox balance and nucleotide biosynthesis. This enzyme supports cellular functions by contributing to the generation of ribose-5-phosphate a precursor for nucleotide synthesis and NADPH essential for anabolic reactions and antioxidative defenses.

Pathways

TKTL2 significantly participates in the pentose phosphate pathway and is implicated in glycolysis connecting to numerous other enzymes like glucose-6-phosphate dehydrogenase. Its activity affects the levels of critical metabolites within these pathways thereby influencing cell survival and proliferation. The interaction of TKTL2 with these pathways highlights its role in regulating cellular metabolic flux impacting processes such as ribonucleotide synthesis and redox homeostasis.

TKTL2 has been associated with cancer particularly in tumor progression and metastasis. Aberrant expression of TKTL2 correlates with altered metabolic states within cancer cells linking it to enhanced tumor growth. It is also suggested that TKTL2 might relate to neurodegenerative conditions where its expression aligns with neuronal metabolic demands and oxidative stress resistance. Research also connects TKTL2 with proteins like lactate dehydrogenase in the context of cancer metabolism illustrating the enzyme's relevance in disease states.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Plays an essential role in total transketolase activity and cell proliferation in cancer cells; after transfection with anti-TKTL1 siRNA, total transketolase activity dramatically decreases and proliferation was significantly inhibited in cancer cells. Plays a pivotal role in carcinogenesis.
See full target information Transketolase-like protein 2
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Journal of lipid research 65:100559 PubMed38729351

2024

Sphingomyelin is involved in regulating UCP1-mediated nonshivering thermogenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Detian Hu,Houyu Zhang,Zhen Liu,Carlos F Ibáñez,Cai Tie,Meng Xie
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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