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Rabbit Recombinant Monoclonal TKTL2 antibody. Suitable for IHC-P, WB and reacts with Human samples.

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Images

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9% PBS, 0.05% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.
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Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PIPFlow CytWB
Human
Tested
Not recommended
Not recommended
Tested
Mouse
Predicted
Not recommended
Not recommended
Predicted
Rat
Predicted
Not recommended
Not recommended
Predicted

Tested
Tested

Species

Human

Dilution info

1/100 - 1/250

Notes

Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.

Predicted
Predicted

Species

Mouse, Rat

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Human, Mouse, Rat

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Human, Mouse, Rat

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

1/1000 - 1/10000

Notes

-

Predicted
Predicted

Species

Mouse, Rat

Dilution info

-

Notes

-

Target data

Function

Plays an essential role in total transketolase activity and cell proliferation in cancer cells; after transfection with anti-TKTL1 siRNA, total transketolase activity dramatically decreases and proliferation was significantly inhibited in cancer cells. Plays a pivotal role in carcinogenesis.

Alternative names

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Rabbit Recombinant Monoclonal TKTL2 antibody. Suitable for IHC-P, WB and reacts with Human samples.

Alternative names

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number

EPR8592

Purification technique

Affinity purification Protein A

Dissociation constant

2.29 x 10-11 M

Concentration
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Storage

Shipped at conditions

Blue Ice

Appropriate long-term storage conditions

-20°C

Storage information

Stable for 12 months at -20°C

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

Biological function summary

TKTL2 contributes to cellular processes related to energy metabolism and biosynthesis. It forms a functional relationship with other proteins in metabolic pathways participating in the regulation of cellular redox balance and nucleotide biosynthesis. This enzyme supports cellular functions by contributing to the generation of ribose-5-phosphate a precursor for nucleotide synthesis and NADPH essential for anabolic reactions and antioxidative defenses.

Activity summary

Transketolase-like 2 (TKTL2) also known as TKL2 is an enzyme involved in carbohydrate metabolism. Its molecular weight is approximately 70 kDa. TKTL2 expression occurs broadly in human tissues with reported high expression levels in the heart brain and liver. The enzyme plays an important role in the non-oxidative part of the pentose phosphate pathway where it influences the interconversion of sugar phosphates.

Pathways

TKTL2 significantly participates in the pentose phosphate pathway and is implicated in glycolysis connecting to numerous other enzymes like glucose-6-phosphate dehydrogenase. Its activity affects the levels of critical metabolites within these pathways thereby influencing cell survival and proliferation. The interaction of TKTL2 with these pathways highlights its role in regulating cellular metabolic flux impacting processes such as ribonucleotide synthesis and redox homeostasis.

Associated diseases and disorders

TKTL2 has been associated with cancer particularly in tumor progression and metastasis. Aberrant expression of TKTL2 correlates with altered metabolic states within cancer cells linking it to enhanced tumor growth. It is also suggested that TKTL2 might relate to neurodegenerative conditions where its expression aligns with neuronal metabolic demands and oxidative stress resistance. Research also connects TKTL2 with proteins like lactate dehydrogenase in the context of cancer metabolism illustrating the enzyme's relevance in disease states.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

3 product images

  • Western blot - Anti-TKTL2 antibody [EPR8592] (ab131331), expandable thumbnail

    Western blot - Anti-TKTL2 antibody [EPR8592] (ab131331)

    All lanes: Western blot - Anti-TKTL2 antibody [EPR8592] (AB131331) at 1/1000 dilution

    Lane 1: Jurkat cell lysate at 10 µg

    Lane 2: HepG2 cell lysate at 10 µg

    Lane 3: BxPC-3 cell lysate at 10 µg

    Lane 4: MCF7 cell lysate at 10 µg

    Secondary

    All lanes: Goat anti-rabbit HRP at 1/2000 dilution

    Predicted band size: 68 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TKTL2 antibody [EPR8592] (ab131331), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TKTL2 antibody [EPR8592] (ab131331)

    Immunohistochemical analysis of paraffin-embedded Human hepatocellular carcinoma tissue labelling TKTL2 with ab131331 at 1/100 dilution.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • OI-RD Scanning - Anti-TKTL2 antibody [EPR8592] (ab131331), expandable thumbnail

    OI-RD Scanning - Anti-TKTL2 antibody [EPR8592] (ab131331)

    We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody.
    Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

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