AB213596

Anti-TLE3/ESG antibody [CL3573]

Name: Anti-TLE3/ESG antibody [CL3573] (ab213596) | Abcam
Brand: Abcam Limited
SKU: AB213596
Price: 525 USD
Availability: InStock

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(2 Publications)

Mouse Monoclonal TLE3/ESG antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human TLE3 aa 150 to C-terminus.

View Alternative Names

KIAA1547, TLE3, Transducin-like enhancer protein 3, Enhancer of split groucho-like protein 3, ESG3

9 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TLE3/ESG antibody [CL3573] (AB213596)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TLE3/ESG antibody [CL3573] (AB213596)

Immunohistochemical analysis of Paraffin-embedded Human cervix tissue labeling TLE3/ESG with ab213596 at 1/10000 dilution.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TLE3/ESG antibody [CL3573] (AB213596)

Immunohistochemical analysis of Paraffin-embedded Human tonsil tissue labeling TLE3/ESG with ab213596 at 1/10000 dilution.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TLE3/ESG antibody [CL3573] (AB213596)

Immunohistochemical analysis of Paraffin-embedded Human small intestine tissue labeling TLE3/ESG with ab213596 at 1/10000 dilution.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TLE3/ESG antibody [CL3573] (AB213596)

Immunohistochemical analysis of Paraffin-embedded Human kidney tissue labeling TLE3/ESG with ab213596 at 1/10000 dilution.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TLE3/ESG antibody [CL3573] (AB213596)

Immunohistochemical analysis of Paraffin-embedded Human oral mucosa tissue labeling TLE3/ESG with ab213596 at 1/10000 dilution.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TLE3/ESG antibody [CL3573] (AB213596)

Immunohistochemical analysis of Paraffin-embedded Human skeletal muscle tissue labeling TLE3/ESG with ab213596 at 1/10000 dilution.

Immunocytochemistry/ Immunofluorescence - Anti-TLE3/ESG antibody [CL3573] (AB213596)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-TLE3/ESG antibody [CL3573] (AB213596)

PFA-fixed, Triton X-100 permeabilized HeLa cells stained for TLE3 (Green) using ab213596 at 4 μg/ml in ICC/IF.

Microtublues are stained red.

Supplier Data

Western blot - Anti-TLE3/ESG antibody [CL3573] (AB213596)

All lanes:

Western blot - Anti-TLE3/ESG antibody [CL3573] (ab213596) at 1/500 dilution

All lanes:

HEK293 cell lysate

Predicted band size: 83 kDa

false

Supplier Data

Western blot - Anti-TLE3/ESG antibody [CL3573] (AB213596)

All lanes:

Western blot - Anti-TLE3/ESG antibody [CL3573] (ab213596) at 1/500 dilution

All lanes:

HeLa nuclear lysate

Predicted band size: 83 kDa

false

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

CL3573

Isotype

IgG1

Carrier free

Reacts with

Human

Applications

IHC-P, ICC/IF, WB

applications

Immunogen

Recombinant Fragment Protein within Human TLE3 aa 150 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q04726

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/10000 - 1/20000", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "2-10 µg/mL", "ICCIF-species-notes": "Fixation/Permeabilization: PFA/Triton X-100" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

TLE3 also known as ESG is a transcriptional corepressor with a molecular mass of around 80 kDa. It belongs to the transducin-like enhancer of split (TLE) family which interacts with DNA-binding proteins to modulate gene transcription. TLE3 is expressed mainly in the nucleus of cells and has notable expression in various tissues including the brain skeletal muscle and adipose tissues. Its mechanical role involves binding to nuclear receptors and influencing the transcriptional repression process by preventing the assembly of transcriptional activators on target genes.

Biological function summary

TLE3 serves as a critical modulator of differentiation pathways. It often functions as part of a broader repressor complex working closely with Groucho-related proteins. These complexes exert influence over cell lineage commitment and differentiation particularly in the context of adipogenesis and muscle development. TLE3 helps direct stem cell fate by repressing pro-differentiation signals maintaining cells in a less differentiated state until specific developmental cues trigger further differentiation.

Pathways

The function of TLE3 intersects with Wnt and Notch signaling pathways both essential for cellular growth and differentiation processes. TLE3 associates with proteins like TCF/LEF during Wnt signaling to suppress target gene activation therefore regulating cellular proliferation and differentiation. In the Notch pathway TLE3 interacts with the CSL transcription factor contributing to the repression of Notch-responsive genes which play a part in the maintenance of stem cell populations and tissue homeostasis.

TLE3 shows a connection with cancer and metabolic syndromes. In cancer aberrant expression of TLE3 can lead to disrupted cell differentiation promoting oncogenesis. For example its interaction with TCF/LEF proteins in the Wnt pathway can affect tumor suppressor gene expression. In metabolic disorders TLE3 impacts adipogenesis contributing to obesity-linked insulin resistance. Dysregulation in these pathways linked to proteins like PPARγ highlights TLE3's role in disease pathophysiology and potential as a therapeutic target.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Transcriptional coregulator that binds to a number of transcription factors. Acts as a regulator of adipogenesis both by acting as a corepressor of Wnt signaling and coactivator for PPARG (PubMed : 28689657). Inhibits the transcriptional activation mediated by CTNNB1 and TCF family members in Wnt signaling (PubMed : 28689657). Promotes diffentiation of white adipocytes by acting as a coactivator for PPARG and preventing the association between PRDM16 and PPARG, thereby preventing browning of white adipocytes (By similarity).

See full target information TLE3

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Genes & development 33:747-762 PubMed31123067

2019

Loss of TLE3 promotes the mitochondrial program in beige adipocytes and improves glucose metabolism.

Applications

Unspecified application

Species

Unspecified reactive species

Stephanie Pearson,Anne Loft,Prashant Rajbhandari,Judith Simcox,Sanghoon Lee,Peter Tontonoz,Susanne Mandrup,Claudio J Villanueva

The Journal of biological chemistry 292:12885-12894 PubMed28607151

2017

The transcriptional co-repressor TLE3 regulates myogenic differentiation by repressing the activity of the MyoD transcription factor.

Applications

Unspecified application

Species

Unspecified reactive species

Shoichiro Kokabu,Chihiro Nakatomi,Takuma Matsubara,Yusuke Ono,William N Addison,Jonathan W Lowery,Mariko Urata,Aaron M Hudnall,Suzuro Hitomi,Mitsushiro Nakatomi,Tsuyoshi Sato,Kenji Osawa,Tetsuya Yoda,Vicki Rosen,Eijiro Jimi

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-TLE3/ESG antibody [CL3573]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TLE3/ESG antibody [CL3573] (AB213596)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TLE3/ESG antibody [CL3573] (AB213596)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TLE3/ESG antibody [CL3573] (AB213596)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TLE3/ESG antibody [CL3573] (AB213596)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TLE3/ESG antibody [CL3573] (AB213596)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TLE3/ESG antibody [CL3573] (AB213596)

Immunocytochemistry/ Immunofluorescence - Anti-TLE3/ESG antibody [CL3573] (AB213596)

Western blot - Anti-TLE3/ESG antibody [CL3573] (AB213596)

Western blot - Anti-TLE3/ESG antibody [CL3573] (AB213596)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

Primary Antibodies

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Primary Antibodies

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

Product promise