AB224729

Anti-TLK2 antibody

Name: Anti-TLK2 antibody (ab224729) | Abcam
Brand: Abcam Limited
SKU: AB224729

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(2 Publications)

Rabbit Polyclonal TLK2 antibody. Suitable for IHC-P, ICC/IF and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human TLK2 aa 1-250.

View Alternative Names

Serine/threonine-protein kinase tousled-like 2, HsHPK, PKU-alpha, Tousled-like kinase 2, TLK2

3 Images

Immunocytochemistry/ Immunofluorescence - Anti-TLK2 antibody (AB224729)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-TLK2 antibody (AB224729)

HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for TLK2 (green) using ab224729 at 1/100 dilution in ICC/IF. Secondary : Alexa Fluor^® 488-conjugated Goat Anti-Rabbit IgG (H+L).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TLK2 antibody (AB224729)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TLK2 antibody (AB224729)

Paraffin-embedded human testis tissue stained for TLK2 with ab224729 at 1/100 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TLK2 antibody (AB224729)

Paraffin-embedded human small intestine tissue stained for TLK2 with ab224729 at 1/100 dilution in immunohistochemical analysis.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IHC-P, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human TLK2 aa 1-250. The exact immunogen used to generate this antibody is proprietary information.

Q86UE8

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/20 - 1/200", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50 - 1/200", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein G

Storage buffer

pH: 7.4 Preservative: 0.03% Proclin 300 Constituents: PBS, 50% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

TLK2 also known as Tousled-like kinase 2 plays a role as a serine/threonine kinase responsible for phosphorylating proteins involved in chromatin remodeling. It has a molecular mass of approximately 90 kDa. TLK2 expresses mainly in proliferative tissues like testes and ovaries highlighting its involvement in cell proliferation processes. This kinase also shows up in other tissues where cell division is active assisting in DNA repair and cell cycle control mechanisms.

Biological function summary

TLK2 acts as a modulator of chromatin assembly by interacting with the ASF1 proteins forming a critical complex in cellular processes. Its activity is essential for the proper progression of the S-phase during the cell cycle. This function connects TLK2 to the maintenance of genomic stability largely through regulating histone incorporation. Such interactions are necessary for the preservation of the genomic integrity during DNA replication.

Pathways

TLK2 significantly impacts cell cycle regulation and DNA damage response pathways. Its participation in the cell cycle pathway intersecting with other kinases is evident as it influences the activity of proteins like CHK1 impacting the G2/M checkpoint. TLK2's involvement in the DNA damage response closely related to the ATM/ATR signaling pathway positions it as an essential player in responding to DNA insults and maintaining cell survival during stress.

TLK2 links closely with cancer especially involving ovarian and testicular tumors where aberrant expression or mutations contribute to uncontrolled cell proliferation. Mutations in TLK2 also have associations with developmental disorders influencing proteins like ASF1B with potential impacts on cognitive development. Understanding TLK2's role in these contexts provides essential insights into potential therapeutic strategies targeting dysregulated cellular processes.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

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Target data

Serine/threonine-protein kinase involved in the process of chromatin assembly and probably also DNA replication, transcription, repair, and chromosome segregation (PubMed : 10523312, PubMed : 11470414, PubMed : 12660173, PubMed : 12955071, PubMed : 29955062, PubMed : 33323470, PubMed : 9427565). Phosphorylates the chromatin assembly factors ASF1A and ASF1B (PubMed : 11470414, PubMed : 20016786, PubMed : 29955062, PubMed : 35136069). Phosphorylation of ASF1A prevents its proteasome-mediated degradation, thereby enhancing chromatin assembly (PubMed : 20016786). Negative regulator of amino acid starvation-induced autophagy (PubMed : 22354037).

See full target information TLK2

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Translational lung cancer research 14:491-512 PubMed40114952

2025

Aspirin impedes non-small cell lung cancer development via fine-tuning the CD36 localization regulated by GPIHBP1.

Applications

Unspecified application

Species

Unspecified reactive species

Wei Liu,Dujuan Qiao,Jia Chen,Ya Gao,Katsuhiro Okuda,Yoshihisa Shimada,Linong Yao

Frontiers in genetics 13:926282 PubMed36134026

2022

Comprehensive analysis of transcriptome characteristics and identification of as a potential biomarker in dermatofibrosarcoma protuberans.

Applications

Unspecified application

Species

Unspecified reactive species

Xiao Zhang,Di Sun,Haiyan Zheng,Yamin Rao,Yuqi Deng,Xiao Liang,Jun Chen,Jun Yang

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-TLK2 antibody

Immunocytochemistry/ Immunofluorescence - Anti-TLK2 antibody (AB224729)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TLK2 antibody (AB224729)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TLK2 antibody (AB224729)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

Product promise