AB213676

Anti-TLR2 antibody

Name: Anti-TLR2 antibody. Rabbit polyclonal (ab213676) | Abcam
Brand: Abcam Limited
SKU: AB213676
Price: 495 USD
Availability: InStock
Rating: 2 (2 reviews)

(2 Reviews)

(45 Publications)

Rabbit Polyclonal TLR2 antibody. Suitable for IHC-P, WB and reacts with Rat, Human, Mouse samples. Cited in 45 publications. Immunogen corresponding to Synthetic Peptide within Human TLR2 aa 700 to C-terminus conjugated to Keyhole Limpet Haemocyanin.

View Alternative Names

CD282, TIL4, TLR2, Toll-like receptor 2, Toll/interleukin-1 receptor-like protein 4

6 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TLR2 antibody (AB213676)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TLR2 antibody (AB213676)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded rat ovary tissue labeling TLR2 with ab213676 at 1/200 dilution, followed by conjugation to the secondary antibody and DAB staining.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TLR2 antibody (AB213676)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded rat ovary tissue labeling TLR2 with ab213676 at 1/200 dilution, followed by conjugation to the secondary antibody and DAB staining.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TLR2 antibody (AB213676)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded rat brain tissue labeling TLR2 with ab213676 at 1/400 dilution, followed by conjugation to the secondary antibody and DAB and counterstaining.

Antigen retrieval was performed by boiling in sodium citrate buffer (0.01M, pH6) for 15 minutes, blocked by endogenous peroxidase by 3% hydrogen peroxide for 30 minutes, blocking buffer (normal goat serum) at 37°C for 20 minutes.

Supplier Data

Western blot - Anti-TLR2 antibody (AB213676)

Incubated with the primary antibody overnight at 4°C.

Incubated with the secondary antibody for 60 minutes at 37°C.

All lanes:

Western blot - Anti-TLR2 antibody (ab213676) at 1/300 dilution

Lane 1:

Mouse spleen tissue lysate

Lane 2:

Mouse thymus tissue lysate

Predicted band size: 89 kDa

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Supplier Data

Western blot - Anti-TLR2 antibody (AB213676)

Incubated with the primary antibody overnight at 4°C.

Incubated with the secondary antibody for 60 minutes at 37°C.

All lanes:

Western blot - Anti-TLR2 antibody (ab213676) at 1/300 dilution

All lanes:

Raw264.7 cell lysate

Predicted band size: 89 kDa

false

Supplier Data

Western blot - Anti-TLR2 antibody (AB213676)

Incubated with the primary antibody overnight at 4°C.

Incubated with the secondary antibody for 60 minutes at 37°C.

All lanes:

Western blot - Anti-TLR2 antibody (ab213676) at 1/1000 dilution

All lanes:

Cerebrum tissue lysate

Predicted band size: 89 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

WB, IHC-P

applications

Immunogen

Synthetic Peptide within Human TLR2 aa 700 to C-terminus conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information.

O60603

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/100 - 1/500", "WB-species-notes": "" }, "Mouse": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/100 - 1/1000", "WB-species-notes": "" }, "Rat": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/500", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cow": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.4 Preservative: 0.02% Proclin 300 Constituents: 50% Glycerol (glycerin, glycerine), 48.98% TBS, 1X, 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Toll-like receptor 2 (TLR2) also called CD282 is a protein that detects molecules from bacteria and fungi. It has a mass of about 90 kDa. TLR2 is expressed on the surface of immune cells such as monocytes macrophages and neutrophils. This receptor recognizes pathogen-associated molecular patterns (PAMPs) and activates the immune response.

Biological function summary

TLR2 initiates signaling pathways that lead to the production of cytokines and chemokines helping to control infection. It forms dimers with other TLRs like TLR1 or TLR6 to expand its recognition range of microbial components. TLR2's activity is important for balancing pathogen recognition and avoiding overactive immune response. On macrophages TLR2 activation enhances their phagocytic ability.

Pathways

TLR2 plays roles in innate immune signaling pathways and inflammation pathways. It contributes significantly to the NF-kB and MAPK pathways which regulate inflammation and immune response. TLR2 often cooperates with proteins like MyD88 and TRAF6 in these pathways leading to downstream activation of transcription factors and production of pro-inflammatory molecules.

TLR2 is associated with inflammatory and infectious diseases including sepsis and tuberculosis. During tuberculosis interactions between TLR2 and its ligands trigger immune responses that are important for bacterial control. Additionally TLR2’s overactivation links to sepsis by causing excessive inflammation. Researchers study TLR2 for potential therapeutic targeting in these diseases assessing how modulation can improve outcomes or reduce detrimental inflammation.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Cooperates with LY96 to mediate the innate immune response to bacterial lipoproteins and other microbial cell wall components. Cooperates with TLR1 or TLR6 to mediate the innate immune response to bacterial lipoproteins or lipopeptides (PubMed : 17889651, PubMed : 21078852). Acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. May also activate immune cells and promote apoptosis in response to the lipid moiety of lipoproteins (PubMed : 10426995, PubMed : 10426996). Recognizes mycoplasmal macrophage-activating lipopeptide-2kD (MALP-2), soluble tuberculosis factor (STF), phenol-soluble modulin (PSM) and B.burgdorferi outer surface protein A lipoprotein (OspA-L) cooperatively with TLR6 (PubMed : 11441107). Stimulation of monocytes in vitro with M.tuberculosis PstS1 induces p38 MAPK and ERK1/2 activation primarily via this receptor, but also partially via TLR4 (PubMed : 16622205). MAPK activation in response to bacterial peptidoglycan also occurs via this receptor (PubMed : 16622205). Acts as a receptor for M.tuberculosis lipoproteins LprA, LprG, LpqH and PstS1, some lipoproteins are dependent on other coreceptors (TLR1, CD14 and/or CD36); the lipoproteins act as agonists to modulate antigen presenting cell functions in response to the pathogen (PubMed : 19362712). M.tuberculosis HSP70 (dnaK) but not HSP65 (groEL-2) acts via this protein to stimulate NF-kappa-B expression (PubMed : 15809303). Recognizes M.tuberculosis major T-antigen EsxA (ESAT-6) which inhibits downstream MYD88-dependent signaling (shown in mouse) (By similarity). Forms activation clusters composed of several receptors depending on the ligand, these clusters trigger signaling from the cell surface and subsequently are targeted to the Golgi in a lipid-raft dependent pathway. Forms the cluster TLR2 : TLR6 : CD14 : CD36 in response to diacylated lipopeptides and TLR2 : TLR1 : CD14 in response to triacylated lipopeptides (PubMed : 16880211). Required for normal uptake of M.tuberculosis, a process that is inhibited by M.tuberculosis LppM (By similarity).

See full target information TLR2

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Publications (45)

Recent publications for all applications. Explore the full list and refine your search

International journal of molecular medicine 56: PubMed40709382

2025

Dulaglutide markedly prevents peritoneal fibrosis in a rodent model of chronic kidney disease: Insights into the pathogenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Chih-Chao Yang,Ya Yue,Yi-Ting Wang,John Y Chiang,Ben-Chung Cheng,Tsuen-Wei Hsu,Yi-Ling Chen,Yi-Chen Li,Hon-Kan Yip

Scientific reports 15:16348 PubMed40348852

2025

TLR2 promotes the progression of diabetes mellitus with atherosclerosis via activating NLRP3 inflammasome and MyD88/NF-κB signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Sisi Chen,Min Xie,Yong Liu

PLoS pathogens 21:e1013041 PubMed40168426

2025

Salmonella Typhi serine threonine kinase T4519 induces lysosomal membrane permeabilization by manipulating Toll-like receptor 2-Cystatin B-Cathepsin B-NF-κB-reactive oxygen species pathway and promotes survival within human macrophages.

Applications

Unspecified application

Species

Unspecified reactive species

Swarnali Chakraborty,Debayan Ganguli,Theeya Nagaraja,Animesh Gope,Sudip Dey,Ananda Pal,Rahul Shubhra Mandal,Sudipta Sekhar Das,Santasabuj Das

British journal of pharmacology 182:2897-2913 PubMed40097259

2025

TLR2 activates AP-1 to facilitate CTGF transcription and stimulate doxorubicin-induced myocardial injury.

Applications

Unspecified application

Species

Unspecified reactive species

Lang Hong,Xinyong Cai,Yuliang Zhan,Songtao Liu,Pengtao Zou,Yanmei Chen,Liang Shao

Journal of cosmetic dermatology 24:e16753 PubMed39823143

2025

Unveiling the Molecular Mechanisms of Rosacea: Insights From Transcriptomics and In Vitro Experiments.

Applications

Unspecified application

Species

Unspecified reactive species

Luzhu Chen,Juan Wang

Journal of central nervous system disease 16:11795735241301568 PubMed39574429

2024

Enriched environment may improve secondary brain injury after traumatic brain injury by regulating the TLR2/NF-κB signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Muyao Wu,Xiaoyi He,Yating Gong,Chaoyu Wang,Yaqian Huang,Fan Gao,Baoqi Dang

Experimental and therapeutic medicine 28:436 PubMed39355523

2024

TLR2 promotes traumatic deep venous thrombosis of the lower extremity following femoral fracture by activating the NF‑κB/COX‑2 signaling pathway in rats.

Applications

Unspecified application

Species

Unspecified reactive species

Tianting Guo,Lijiao Xiong,Junbin Xie,Jiwei Zeng,Zhihua Huang,Mengting Yao,Xiaoan Zhang,Jianwen Mo

Veterinary research 55:93 PubMed39075605

2024

HbpA from Glaesserella parasuis induces an inflammatory response in 3D4/21 cells by activating the MAPK and NF-κB signalling pathways and protects mice against G. parasuis when used as an immunogen.

Applications

Unspecified application

Species

Unspecified reactive species

Zhen Yang,Yiwen Zhang,Qin Zhao,Senyan Du,Xiaobo Huang,Rui Wu,Qigui Yan,Xinfeng Han,Yiping Wen,San-Jie Cao

Histology and histopathology 39:623-632 PubMed37916940

2023

mitigates autoimmune hepatitis by regulating IL-33-induced Treg/Th17 imbalance via the TLR2/4 signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Jianguo Song,Juan Dai,Xueping Chen,Fei Ding,Yanbo Ding,Liang Ma,Liwen Zhang

British journal of pharmacology 180:3215-3233 PubMed37519261

2023

Use of Saccharomyces boulardii CNCM I-745 as therapeutic strategy for prevention of nonsteroidal anti-inflammatory drug-induced intestinal injury.

Applications

Unspecified application

Species

Unspecified reactive species

Vanessa D'Antongiovanni,Luca Antonioli,Laura Benvenuti,Carolina Pellegrini,Clelia Di Salvo,Marco Calvigioni,Adelaide Panattoni,Larisa Ryskalin,Gianfranco Natale,Sebastiano Banni,Gianfranca Carta,Emilia Ghelardi,Matteo Fornai

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