Mouse Monoclonal TLR2 antibody. Suitable for Flow Cyt, FuncS and reacts with Mouse samples. Cited in 33 publications. Immunogen corresponding to Synthetic Peptide within Mouse Tlr2.
Images
![Flow Cytometry - Anti-TLR2 antibody [T2.5] (AB16894), expandable thumbnail](https://content.abcam.com/products/images/tlr2-antibody-t25-ab16894--flow-cytometry-img122477.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry - Anti-TLR2 antibody [T2.5] (AB16894)")
![Functional Studies - Anti-TLR2 antibody [T2.5] (AB16894), expandable thumbnail](https://content.abcam.com/products/images/tlr2-antibody-t25-ab16894--functional-studies-img84220.jpg/_jcr_content/renditions/webp-475.webp "Functional Studies - Anti-TLR2 antibody [T2.5] (AB16894)")
Publications
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- Journal of cellular physiology 238:1507-15192023IFI16/Ifi202 released from breast cancer induces secretion of inflammatory cytokines from macrophages and promotes tumor growth.Applications:Unspecified applicationReactive species:Unspecified reactive speciesGa Young Lim,Sun Wook Cho,Na-Lee Ka,Kyung-Hun Lee,Seock-Ah Im,Seung-Su Kim,Sewon Hwang,Mi-Ock LeePubMed 37062941
- Neural regeneration research 18:1371-13772022Argon preconditioning protects neuronal cells with a Toll-like receptor-mediated effect.Applications:Unspecified applicationReactive species:Unspecified reactive speciesStefanie Scheid,Adrien Lejarre,Jakob Wollborn,Hartmut Buerkle,Ulrich Goebel,Felix UlbrichPubMed 36453425
- The Journal of biological chemistry 298:1018572022The transmembrane adapter SCIMP recruits tyrosine kinase Syk to phosphorylate Toll-like receptors to mediate selective inflammatory outputs.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLiping Liu,Richard M Lucas,Jeffrey D Nanson,Yan Li,Jason Whitfield,James E B Curson,Neeraj Tuladhar,Kirill Alexandrov,Mehdi Mobli,Matthew J Sweet,Bostjan Kobe,Jennifer L Stow,Lin LuoPubMed 35337798
- Frontiers in immunology 12:7490742021Complement-Mediated Differential Immune Response of Human Macrophages to Species Through Interaction With Their Cell Wall Peptidorhamnomannans.Applications:Unspecified applicationReactive species:Unspecified reactive speciesGabriela W P Neves,Sarah Sze Wah Wong,Vishukumar Aimanianda,Catherine Simenel,J Iñaki Guijarro,Catriona Walls,Janet A Willment,Neil A R Gow,Carol A Munro,Gordon D Brown,Leila M Lopes-BezerraPubMed 34867977
- BMC medicine 19:2472021HBeAg mediates inflammatory functions of macrophages by TLR2 contributing to hepatic fibrosis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXiaoyu Xie,Huanran Lv,Chenxi Liu,Xiaonan Su,Zhen Yu,Shouyang Song,Hongjun Bian,Miaomiao Tian,Chengyong Qin,Jianni Qi,Qiang ZhuPubMed 34649530
- Experimental and therapeutic medicine 22:10812021Herbal melanin induces interleukin-1β secretion and production by human THP-1 monocytes via Toll-like receptor 2 and p38 MAPK activation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAdila El-Obeid,Wesam Bin Yahya,Bader Almuzzaini,Abeer Al Tuwaijri,Maria Najdi,Adil Hassib,Sabine Matou-NasriPubMed 34447474
- PLoS biology 19:e30009392021Extracellular DJ-1 induces sterile inflammation in the ischemic brain.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKoutarou Nakamura,Seiichiro Sakai,Jun Tsuyama,Akari Nakamura,Kento Otani,Kumiko Kurabayashi,Yoshiko Yogiashi,Hisao Masai,Takashi ShichitaPubMed 34014921
- Molecular medicine reports 23:2021Rs41291957 polymorphism in the promoter region of microRNA‑143 serves as a prognostic biomarker for patients with intracranial hemorrhage.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXiaobo Yang,Zongduo Guo,Fang Cao,Zhipeng Teng,Zhijian Huang,Xiaochuan SunPubMed 33649782
- Medicine 100:e239862021Paeoniflorin inhibits the macrophage-related rosacea-like inflammatory reaction through the suppressor of cytokine signaling 3-apoptosis signal-regulating kinase 1-p38 pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZijing Liu,Jiawen Zhang,Peiyu Jiang,Zhi Yin,Yunyi Liu,Yixuan Liu,Xiaoyan Wang,Liang Hu,Yang Xu,Wentao LiuPubMed 33545988
- Scientific reports 11:2222021Platelet CXCL4 mediates neutrophil extracellular traps formation in ANCA-associated vasculitis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKotaro Matsumoto,Hidekata Yasuoka,Keiko Yoshimoto,Katsuya Suzuki,Tsutomu TakeuchiPubMed 33420306
Key facts
- Isotype
- IgG1
- Host species
- Mouse
- Storage buffer
Constituents: PBS, 0.1% BSA
- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- Synthetic Peptide within Mouse Tlr2. The exact immunogen used to generate this antibody is proprietary information. Database link Q9QUN7
Abcam Recommends
Reactivity data
Select an application| Flow Cyt | WB | FuncS | |
|---|---|---|---|
| Human | Predicted | Not recommended | Predicted |
| Mouse | Tested | Not recommended | Tested |
Flow Cyt
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. 4 x 104 leukocytes/ml were stained for 30 minutes at 4 °C. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
WB
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse | Dilution info - | Notes - |
FuncS
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes Mice were injected i.p. with 1 mg ab16894, after 1 hour incubation mice were challenged; 5 μg/ml was added to cell culture. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Associated Products
Select an associated product type
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Target data
Function
Cooperates with LY96 to mediate the innate immune response to bacterial lipoproteins and other microbial cell wall components. Cooperates with TLR1 or TLR6 to mediate the innate immune response to bacterial lipoproteins or lipopeptides (PubMed:17889651, PubMed:21078852). Acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. May also activate immune cells and promote apoptosis in response to the lipid moiety of lipoproteins (PubMed:10426995, PubMed:10426996). Recognizes mycoplasmal macrophage-activating lipopeptide-2kD (MALP-2), soluble tuberculosis factor (STF), phenol-soluble modulin (PSM) and B.burgdorferi outer surface protein A lipoprotein (OspA-L) cooperatively with TLR6 (PubMed:11441107). Stimulation of monocytes in vitro with M.tuberculosis PstS1 induces p38 MAPK and ERK1/2 activation primarily via this receptor, but also partially via TLR4 (PubMed:16622205). MAPK activation in response to bacterial peptidoglycan also occurs via this receptor (PubMed:16622205). Acts as a receptor for M.tuberculosis lipoproteins LprA, LprG, LpqH and PstS1, some lipoproteins are dependent on other coreceptors (TLR1, CD14 and/or CD36); the lipoproteins act as agonists to modulate antigen presenting cell functions in response to the pathogen (PubMed:19362712). M.tuberculosis HSP70 (dnaK) but not HSP65 (groEL-2) acts via this protein to stimulate NF-kappa-B expression (PubMed:15809303). Recognizes M.tuberculosis major T-antigen EsxA (ESAT-6) which inhibits downstream MYD88-dependent signaling (shown in mouse) (By similarity). Forms activation clusters composed of several receptors depending on the ligand, these clusters trigger signaling from the cell surface and subsequently are targeted to the Golgi in a lipid-raft dependent pathway. Forms the cluster TLR2:TLR6:CD14:CD36 in response to diacylated lipopeptides and TLR2:TLR1:CD14 in response to triacylated lipopeptides (PubMed:16880211). Required for normal uptake of M.tuberculosis, a process that is inhibited by M.tuberculosis LppM (By similarity).
Alternative names
CD282, TIL4, TLR2, Toll-like receptor 2, Toll/interleukin-1 receptor-like protein 4
Recommended products
Mouse Monoclonal TLR2 antibody. Suitable for Flow Cyt, FuncS and reacts with Mouse samples. Cited in 33 publications. Immunogen corresponding to Synthetic Peptide within Mouse Tlr2.
Key facts
- Isotype
- IgG1
- Host species
- Mouse
- Storage buffer
Constituents: PBS, 0.1% BSA
- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- Synthetic Peptide within Mouse Tlr2. The exact immunogen used to generate this antibody is proprietary information. Database link Q9QUN7
- Clone number
- T2.5
- Purification technique
- Affinity purification Protein G
- Specificity
The endotoxin concentration is < 24 EU/mg, determined with HIT302 LAL Assay. Negative control: CHO cells.
- Concentration
- Purification notes
0.2 µm filtered
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Toll-like receptor 2 (TLR2) also called CD282 is a protein that detects molecules from bacteria and fungi. It has a mass of about 90 kDa. TLR2 is expressed on the surface of immune cells such as monocytes macrophages and neutrophils. This receptor recognizes pathogen-associated molecular patterns (PAMPs) and activates the immune response.
- Biological function summary
TLR2 initiates signaling pathways that lead to the production of cytokines and chemokines helping to control infection. It forms dimers with other TLRs like TLR1 or TLR6 to expand its recognition range of microbial components. TLR2's activity is important for balancing pathogen recognition and avoiding overactive immune response. On macrophages TLR2 activation enhances their phagocytic ability.
- Pathways
TLR2 plays roles in innate immune signaling pathways and inflammation pathways. It contributes significantly to the NF-kB and MAPK pathways which regulate inflammation and immune response. TLR2 often cooperates with proteins like MyD88 and TRAF6 in these pathways leading to downstream activation of transcription factors and production of pro-inflammatory molecules.
- Associated diseases and disorders
TLR2 is associated with inflammatory and infectious diseases including sepsis and tuberculosis. During tuberculosis interactions between TLR2 and its ligands trigger immune responses that are important for bacterial control. Additionally TLR2’s overactivation links to sepsis by causing excessive inflammation. Researchers study TLR2 for potential therapeutic targeting in these diseases assessing how modulation can improve outcomes or reduce detrimental inflammation.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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2 product images
![Flow Cytometry - Anti-TLR2 antibody [T2.5] (ab16894), expandable thumbnail](https://content.abcam.com/products/images/tlr2-antibody-t25-ab16894--flow-cytometry-img122477.jpg "Flow Cytometry - Anti-TLR2 antibody [T2.5] (ab16894)")
Flow Cytometry - Anti-TLR2 antibody [T2.5] (ab16894)
Detection of TLR2 in RAW cells. Red, black and blue line represent the isotype control, cells only and ab16894 at 10 μg/ml, respectively.
![Functional Studies - Anti-TLR2 antibody [T2.5] (ab16894), expandable thumbnail](https://content.abcam.com/products/images/tlr2-antibody-t25-ab16894--functional-studies-img84220.jpg "Functional Studies - Anti-TLR2 antibody [T2.5] (ab16894)")
Functional Studies - Anti-TLR2 antibody [T2.5] (ab16894)
Effect of anti-TLR antibodies on TNF production in a whole blood model.
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