AB137722

Anti-TLR3 antibody

Name: Anti-TLR3 antibody (ab137722) | Abcam
Brand: Abcam Limited
SKU: AB137722
Price: 565 USD
Availability: InStock
Rating: 5 (1 reviews)

(1 Review)

(6 Publications)

Rabbit Polyclonal TLR3 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Mouse, Rat, Human samples. Cited in 6 publications. Immunogen corresponding to Recombinant Fragment Protein within Human TLR3 aa 450-650.

View Alternative Names

CD283, Toll-like receptor 3, TLR3

5 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TLR3 antibody (AB137722)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TLR3 antibody (AB137722)

Immunohistochemical analysis of paraffin-embedded TOV-112D xenograft labelling TLR3 with ab137722 at 1/100 dilution.

Antigen Retrieval : Citrate buffer, pH 6.0, 15 min.

Immunocytochemistry/ Immunofluorescence - Anti-TLR3 antibody (AB137722)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-TLR3 antibody (AB137722)

Immunofluorescenct analysis of methanol-fixed HeLa cells labelling TLR3 with ab137722 at 1/200 dilution. Lower image is co-stained with Hoechst 33342.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TLR3 antibody (AB137722)

Immunohistochemical analysis of paraffin-embedded rat pancreas tissue labelling TLR3 at cytoplasm with ab137722 at 1/1000 dilution.

Antigen Retrieval : Citrate buffer, pH 6.0, 15 min.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TLR3 antibody (AB137722)

Immunohistochemical analysis of paraffin-embedded mouse liver tissue labelling TLR3 at cytoplasm with ab137722 at 1/1000 dilution.

Antigen Retrieval : Citrate buffer, pH 6.0, 15 min.

Unknown

Western blot - Anti-TLR3 antibody (AB137722)

5% SDS PAGE

All lanes:

Western blot - Anti-TLR3 antibody (ab137722) at 1/5000 dilution

Lane 1:

K562 whole cell lysate at 30 µg

Lane 2:

THP-1 whole cell lysate at 30 µg

Predicted band size: 104 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

ICC/IF, IHC-P, WB

applications

Immunogen

Recombinant Fragment Protein within Human TLR3 aa 450-650. The exact immunogen used to generate this antibody is proprietary information.

O15455

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "" }, "Mouse": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 Preservative: 0.025% Proclin 300 Constituents: PBS, 20% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

TLR3 also known as Toll-like receptor 3 is a protein that plays an important role in immune response mechanisms. With a molecular mass of around 104 kDa TLR3 is part of the Toll-like receptor family and expresses mostly in immune cells including dendritic cells and macrophages. It locates mainly on the endosomal membrane where it recognizes double-stranded RNA (dsRNA) which is often a sign of viral infection. TLR3 initiates signaling cascades that lead to the activation of important immune transcription factors.

Biological function summary

TLR3 acts as a sensor for viral and endogenous dsRNA. This receptor forms part of the innate immune system serving as a first line of defense against microbial infections. Upon encountering its ligand TLR3 undergoes conformational changes which allow interaction with the adaptor protein TRIF (TIR-domain-containing adapter-inducing interferon-β). This interaction triggers a downstream signaling cascade that leads to the production of type I interferons and pro-inflammatory cytokines which are essential for mounting an effective antiviral response.

Pathways

TLR3 engages in important signaling cascades like the NF-kB and IRF3 pathways. These pathways are activated by the TRIF-dependent pathway which is unique among the Toll-like receptors. TLR3 influences the expression of genes involved in the inflammatory response and apoptosis. The interactions of TLR3 with other proteins such as MyD88 and IRAK1 are indirect as TLR3 does not depend on the usual MyD88-dependent pathway. This specificity makes TLR3 a critical component in pathogen recognition and response strategies within the immune system.

TLR3 has connections with several conditions primarily viral infections and autoimmune disorders like systemic lupus erythematosus (SLE). Mutations or dysregulation of TLR3 may lead to altered immune responses contributing to the pathogenesis of these diseases. TLR3's association with proteins like TRIF and IRF3 is significant in understanding its role in these conditions. Ongoing research investigates TLR3's potential as a therapeutic target to modulate immune responses in disease states.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Key component of innate and adaptive immunity. TLRs (Toll-like receptors) control host immune response against pathogens through recognition of molecular patterns specific to microorganisms. TLR3 is a nucleotide-sensing TLR which is activated by double-stranded RNA, a sign of viral infection. Acts via the adapter TRIF/TICAM1, leading to NF-kappa-B activation, IRF3 nuclear translocation, cytokine secretion and the inflammatory response.

See full target information TLR3

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Publications (6)

Recent publications for all applications. Explore the full list and refine your search

Cell reports. Medicine 5:101782 PubMed39413734

2024

Targeting exosomal double-stranded RNA-TLR3 signaling pathway attenuates morphine tolerance and hyperalgesia.

Applications

Unspecified application

Species

Unspecified reactive species

Bing Wang,Dong-Sheng Le,Li Liu,Xue-Xue Zhang,Fan Yang,Guo-Rong Lai,Chao Zhang,Mai-Lin Zhao,Yun-Peng Shen,Ping-Sheng Liao,Tong Liu,Ying-Ping Liang

American journal of physiology. Gastrointestinal and liver physiology 327:G267-G283 PubMed38860860

2024

Specific binding sites on Rhesus rotavirus capsid protein dictate the method of endocytosis inducing the murine model of biliary atresia.

Applications

Unspecified application

Species

Unspecified reactive species

Haley Temple,Bryan Donnelly,Sujit K Mohanty,Sarah Mowery,Holly M Poling,Rajamouli Pasula,Stephen Hartman,Akaljot Singh,Reena Mourya,Alexander Bondoc,Jaroslaw Meller,Anil G Jegga,Kei Oyama,Monica McNeal,Paul Spearman,Greg Tiao

Advances in clinical and experimental medicine : official organ Wroclaw Medical University : PubMed37486697

2023

The long non-coding RNA BDNF-AS induces neuronal cell apoptosis by targeting miR-125b-5p in Alzheimer's disease models.

Applications

Unspecified application

Species

Unspecified reactive species

Haiyan Ren,Weibin Qiu,Benju Zhu,Qiang Li,Chen Peng,Xu Chen

Acta pharmaceutica Sinica. B 12:3667-3681 PubMed36176917

2022

Small molecule SMU-CX24 targeting toll-like receptor 3 counteracts inflammation: A novel approach to atherosclerosis therapy.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaohong Cen,Baoqu Wang,Yuqing Liang,Yanlin Chen,Yu Xiao,Shaohua Du,Kutty Selva Nandakumar,Hang Yin,Shuwen Liu,Kui Cheng

ACS nano 11:12145-12155 PubMed29016111

2017

Crystallinity of Double-Stranded RNA-Antimicrobial Peptide Complexes Modulates Toll-Like Receptor 3-Mediated Inflammation.

Applications

Unspecified application

Species

Unspecified reactive species

Ernest Y Lee,Toshiya Takahashi,Tine Curk,Jure Dobnikar,Richard L Gallo,Gerard C L Wong

BioMed research international 2016:9872138 PubMed28105439

2016

Functional Roles of Pattern Recognition Receptors That Recognize Virus Nucleic Acids in Human Adipose-Derived Mesenchymal Stem Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Lili Yu,Yongtao Xu,Fangchao Wang,Can Yang,Guoyan Liu,Xiangfeng Song

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-TLR3 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TLR3 antibody (AB137722)

Immunocytochemistry/ Immunofluorescence - Anti-TLR3 antibody (AB137722)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TLR3 antibody (AB137722)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TLR3 antibody (AB137722)

Western blot - Anti-TLR3 antibody (AB137722)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (6)

Product promise